Alpha-actinin-4 / ACTN4 antibody

Principal name

Alpha-actinin-4 / ACTN4 antibody

Alternative names for Alpha-actinin-4 / ACTN4 antibody

Non-muscle alpha actinin 4, F-actin cross-linking protein

SwissProt ID

A5D7D1 (Bovin), O43707 (Human), P57780 (Mouse), Q90734 (Chick), Q9QXQ0 (Rat)

Gene ID

81 (ACTN4), 1488781 (V82L)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Alpha-actinin-4 / ACTN4 antibody

Alpha actinins (Actn's) are known for their ability to modulate cytoskeletal organization and cell motility by cross-linking actin filaments. There are several actin binding proteins including MACF1, alpha actinins, cofilin, fascin and elongation factor 1 alfa 2 (eEF1A2) working in concert for cell viability, migration and proliferation. Alfa actinins belong to a spectrin gene superfamily that represents a wide range of cytoskeletal proteins including alfa and beta spectrin and dystrophins. The alfa actinins play different role in different type of cells, in non-muscle cells, this protein is found along the microfilament boundless and adherens-type junctions. The actinins involves in binding of actin to the membrane. In muscle cells (skeletal, cardiac, and smooth muscle isoforms) the protein ins localized in z-disc and along the dense bodies where they anchor the myofrillar actin filaments. Alfa Actinin 4 is a nonmuscle isoform that concentrated in the cytoplasm and is involved in the metastatic processes. It is essential for maintaining the spreading motility and contractility of fibtroblasts (2). Mutations in this gene are associated with glomerulosclerosis and other kidney dysfunctions (1). The actinin4 gene is localized on chromosome 19q13, the protein has several functional motifs including, calponin homology domain, PIP2 binding site, actin binding domain, several spectrin repeats and calcium binding domain. Alfa-actining 4 has a putative phosphorylation site at Serine 159. Actn 4 is phosphorylated by growth factor stimulation and upon phosphorylation its affinity for actin is reduced. The levels of Alfa actinin 4 is significantly increased in advanced and metastatic human ovarian carcinoma leading to increased invasion and motility of ovarian cancer cells. The alfa-Actinin4-selective antibodies were generated using a peptide selected form region between amino acids 140-190. The Actn4 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer for long-term storage.

General readings

Celli L. et al. Evidence of a Functional Role for Interaction between ICAM-1 and Nonmuscle -Actinins in Leukocyte Diapedesis. The Journal of Immunology; 2006, 177: 4113-4121.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Actinin-4 Splice Variant antibody

Actinin-4 Splice Variant Mouse 15H2 Purified Hu P, WB
50 µg / €760.00
  Funakoshi Co., Ltd.

Alpha-actinin-4 / ACTN4 antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308476 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-4 / ACTN4 antibody

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4D10 Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Alpha-actinin-4 / ACTN4 antibody

Immunoperoxidase of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml] Mouse IgG2a 4D10 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Western blot analysis of extracts from 293 cells, HeLa cells and A549 cells, using ACTN4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-4 / ACTN4 (N-term) antibody

Immunohistochemistry on breast carcinoma Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-actinin-4 / ACTN4 (C-term) antibody

(LEFT) Western blot analysis of ACTN4 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). ACTN4(arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-ACTN4 Antibody (C-term) in mouse bladder tissue lysates (35ug/lane). ACTN4(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Rabbit Purified Hu C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ACTN4 and CTNNB1. HeLa cells were stained with anti-ACTN4 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG2a 4D10 Ascites Hu, Ms, Rt E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG2a 1E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG1 8G12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG1 8G4 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG1 8C1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG1 7F1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG1 3G12 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG1 1F8 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG1 1F20 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-4 / ACTN4 antibody

Alpha-actinin-4 / ACTN4 Mouse IgG1 3G24 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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