Alpha-Adducin / ADD1 antibody

Principal name

Alpha-Adducin / ADD1 antibody

Alternative names for Alpha-Adducin / ADD1 antibody

ADDA, Erythrocyte adducin subunit alpha

SwissProt ID

P35611 (Human), Q63028 (Rat), Q9QYC0 (Mouse)

Gene ID

118 (ADD1), 11518 (Add1), 24170

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of Alpha-Adducin / ADD1 antibody

The α-adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca2+/calmodulin and acts as a substrate for protein kises A and C. α-addducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers α/β, and α/γ. The molecular weights for the various isoforms are α=120 kD; β=110kD; γ=80kD. This protein has been reported to promote the assembly of the spectrin-actin network. Polymorphisms in α-adducin are associated with cardiovascular disease and hypertension. α-adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kise, α-adducin can regulate membrane ruffling and cell motility. The Poly6170 antibody has been shown to be useful for Western blotting of mouse and human α-adducin protein.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-Adducin / ADD1 antibody

  Rabbit anti-ADD1 (Phospho-Ser726) polyclonal antibody(Phospho-specific)  
Western blot analysis of extracts from HT29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 antibody (Lanes 1 and 2) and ADD1 (phospho-Ser726) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  Rabbit polyclonal ADD1 (Ser726) antibody(Phospho-specific)  
Western blot analysis of extracts from Hela cells treated with Forskolin (40nM, 30mins), using ADD1 (phospho-Ser726) antibody (#TA313191).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  Rabbit polyclonal ADD1 (Ab-726) antibody  
Western blot analysis of extracts from Hela cells treated with Forskolin (40nM, 30min), using ADD1 (Ab-726) antibody (#TA313192, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  Anti-ADD1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ADD1 antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  Anti-ADD1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human cervical and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue  using ADD1 antibody at dilution 1/50, on the right is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ADD1 antibody at dilution 1/50. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  Anti-ADD1 (Phospho-Ser726) Rabbit Polyclonal Antibody  
Predicted band size: 130 kDa . Positive control: Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Treated with the peptide Lane2: Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  Rabbit Polyclonal ADD1 Antibody  
Western blot analysis of ADD1 expression in Forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  ADD1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of BT474 and mouse brain cell lines, using ADD1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  Rabbit Polyclonal anti-a-Adducin Antibody  
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-α-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6170 Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

  Rabbit Polyclonal anti-a-Adducin Antibody  
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-alpha;-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit Poly6170 Purified Hu, Ms ICC/IF, WB
50 µl / €248.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 pSer726 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) antibody AP02458PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 pSer726 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) antibody AP02458PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 antibody

Immunohistochemical analysis of paraffin-emebedded human lung carcinoma tissue using ADD1 Antibody (left) or the same antibody preincubated with  blocking peptide (right) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 antibody

Immunohistochemical analysis of paraffin-emebedded human lung carcinoma tissue using ADD1 Antibody (left) or the same antibody preincubated with  blocking peptide (right) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 antibody

Immunohistochemistry analysis of ADD1 Antibody (Cat.-No AP06003PU-N) antibody in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt E, IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 antibody

Immunohistochemistry (IHC) analysis of ADD1 antibody (Cat.-No.: AP06423PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 pSer726 antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 pSer726 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

Alpha-Adducin / ADD1 pSer726 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

Alpha-Adducin / ADD1 pThr445 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Thr445) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €230.00
  Assay Biotech

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