Alpha-Adducin / ADD1 antibody

Principal name

Alpha-Adducin / ADD1 antibody

Alternative names for Alpha-Adducin / ADD1 antibody

ADDA, Erythrocyte adducin subunit alpha

SwissProt ID

P35611 (Human), Q63028 (Rat), Q9QYC0 (Mouse)

Gene ID

118 (ADD1), 11518 (Add1), 24170

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of Alpha-Adducin / ADD1 antibody

The α-adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca2+/calmodulin and acts as a substrate for protein kises A and C. α-addducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers α/β, and α/γ. The molecular weights for the various isoforms are α=120 kD; β=110kD; γ=80kD. This protein has been reported to promote the assembly of the spectrin-actin network. Polymorphisms in α-adducin are associated with cardiovascular disease and hypertension. α-adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kise, α-adducin can regulate membrane ruffling and cell motility. The Poly6170 antibody has been shown to be useful for Western blotting of mouse and human α-adducin protein.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-adducin pSer726/pSer713 antibody

Immunofluorescent analysis of Alpha-adducin (pS726/713) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Por, Rt ICC/IF, IP, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

Alpha-adducin pSer726/pSer713 antibody

Immunofluorescent analysis of Alpha-adducin (pS726/713) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Por, Rt ICC/IF, IP, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

Alpha-adducin pSer726/pSer713 antibody

Immunofluorescent analysis of Alpha-adducin (pS726/713) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Por, Rt ICC/IF, IP, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

Alpha-adducin antibody

Immunohistochemical analysis of Alpha-adducin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Alpha-adducin antibody

Immunohistochemical analysis of Alpha-adducin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Alpha-adducin antibody

Immunohistochemical analysis of Alpha-adducin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Alpha-Adducin / ADD1 antibody

Western blot analysis of extracts from HT29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 antibody (Lanes 1 and 2) and ADD1 (phospho-Ser726) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

Western blot analysis of extracts from Hela cells treated with Forskolin (40nM, 30mins), using ADD1 (phospho-Ser726) antibody (#TA313191).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

Western blot analysis of extracts from Hela cells treated with Forskolin (40nM, 30min), using ADD1 (Ab-726) antibody (#TA313192, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ADD1 antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue  using ADD1 antibody at dilution 1/50, on the right is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ADD1 antibody at dilution 1/50. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

Predicted band size: 130 kDa . Positive control: Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Treated with the peptide Lane2: Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

Western blot analysis of ADD1 expression in Forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

Western blot analysis of extracts of BT474 and mouse brain cell lines, using ADD1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-α-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6170 Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 antibody

HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-alpha;-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit Poly6170 Purified Hu, Ms ICC/IF, WB
50 µl / €248.00
  OriGene Technologies, Inc.

Alpha-Adducin / ADD1 pSer726 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) antibody AP02458PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 pSer726 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) antibody AP02458PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 antibody

Immunohistochemical analysis of paraffin-emebedded human lung carcinoma tissue using ADD1 Antibody (left) or the same antibody preincubated with  blocking peptide (right) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Alpha-Adducin / ADD1 antibody

Immunohistochemical analysis of paraffin-emebedded human lung carcinoma tissue using ADD1 Antibody (left) or the same antibody preincubated with  blocking peptide (right) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

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