Alpha-crystallin A chain / CRYA1 antibody

Principal name

Alpha-crystallin A chain / CRYA1 antibody

Alternative names for Alpha-crystallin A chain / CRYA1 antibody

CRYAA, HSPB4, Heat shock protein beta-4

SwissProt ID

P02470 (Bovin), P02475 (Pig), P02489 (Human), P24622 (Mouse), P24623 (Rat), P68280 (Canfa)

Gene ID

102724652 (LOC102724652), 24273 (Cryaa), 12954

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Alpha-crystallin A chain / CRYA1 antibody

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-termil extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not reture proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translatiol modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additiol functions of alpha crystallins are an autokise activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal domint congenital cataract (ADCC). [provided by RefSeq, Jul 2008].

General readings

General / background references:Laksanalamai P & Robb FT Small heat shock proteins from extremophiles: a review. Extremophiles 8:1-11 (2004). PubMed PMID: 15064984

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-crystallin A chain / CRYA1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505453) Mouse IgG2b OTIC3 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin A chain / CRYA1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRYAA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505577) Mouse IgG2b OTI3B12 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin A chain / CRYA1 antibody

Western blot analysis of AlphaA Crystallin in bovine tissues at a 1:1000 dilution of the antibody Mouse IgG1 1H3.B8 Aff - Purified Bov, Hu, Ms, Rt E, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Alpha-crystallin A chain / CRYA1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAA (RC216946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAA. Mouse IgG1 OTIH2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin A chain / CRYA1 antibody

Western blot analysis of AlphaA Crystallin in bovine tissues at a 1:1000 dilution of the antibody Mouse IgG1 1H3.B8 Aff - Purified Bov, Hu, Ms, Rt E, WB
50 µg / €220.00
  Acris Antibodies GmbH

Alpha-crystallin A chain / CRYA1 antibody

Western blot analysis: Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. This antibody does not show cross-activity about Crystallin alpha B.            Lane A: Crystallin-alpha A recombinant protein, Lane B: Crystallin-alpha B recombinant protein, Lane C: Mouse eye lysates Mouse IgG1 c9F2 Purified Hu, Ms, Rt E, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

Alpha-crystallin A chain / CRYA1 antibody

Western blot analysis: Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. This antibody does not show cross-activity about Crystallin alpha B.            Lane A: Crystallin-alpha A recombinant protein, Lane B: Crystallin-alpha B recombinant protein, Lane C: Mouse eye lysates Mouse IgG1 c9F2 Purified Hu, Ms, Rt E, WB
50 µl / €230.00
  Acris Antibodies GmbH

Alpha-crystallin A chain / CRYA1 antibody

Immunoblot analysis of rat eye extracts were resolved by electrophoresis , transferred to PVDF membrane and probed with CRYAA monoclonal antibody , clone c9F2 ( 1 : 1000 ) ( Cat # MAB1054 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates CRYAA ( ~ 20 kDa ) . Mouse IgG1 c9F2 Hu, Rt E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Alpha-crystallin A chain / CRYA1 antibody

Alpha-crystallin A chain / CRYA1 Mouse IgG1 1H3.B8 Bov, Hu, Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

Alpha-crystallin A chain / CRYA1 antibody

Western Blot: alpha A Crystallin Antibody [NB120-5595] - Figure 1 illustrates Western blot detection of alpha-A crystallin from bovine lens extract using NB120-5595. Rabbit IgG Aff - Purified Bov, Rt P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Alpha-crystallin A chain / CRYA1 antibody

Alpha-crystallin A chain / CRYA1 Mouse IgG1 c9F2 Purified Hu, Rt E, WB
0.1 ml / €550.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Alpha-crystallin A chain / CRYA1

Alpha-crystallin A chain / CRYA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Alpha-crystallin A chain / CRYA1 (1-175)

Alpha-crystallin A chain / CRYA1 Mouse Purified > 95 % > 95 % by SDS PAGE E. coli
0.5 mg / €760.00
  Acris Antibodies GmbH

Alpha-crystallin A chain / CRYA1 (1-175)

Alpha-crystallin A chain / CRYA1 Mouse Purified > 95 % > 95 % by SDS PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Alpha-crystallin A chain / CRYA1

Recombinant human CRYAA: 14% SDS-PAGE Human Purified ≥ 95 by SDS-PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

Alpha-crystallin A chain / CRYA1

Recombinant human CRYAA: 14% SDS-PAGE Human Purified > 95 % by SDS-PAGE E. coli
1 mg / €590.00
  Acris Antibodies GmbH

Alpha-crystallin A chain / CRYA1

Alpha-crystallin A chain / CRYA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Alpha-crystallin A chain / CRYA1

Alpha-crystallin A chain / CRYA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Alpha-crystallin A chain / CRYA1

SDS-Page: alpha A Crystallin Protein [NBC1-18351] - Crystallin alpha A, 19.9kDa (173aa) with a purity of 95% by SDS - PAGE Protein
0.1 mg / €310.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

CRYAA overexpression lysate

CRYAA overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

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