Alpha-crystallin B chain / CRYA2 antibody

Principal name

Alpha-crystallin B chain / CRYA2 antibody

Alternative names for Alpha-crystallin B chain / CRYA2 antibody

Alpha(B)-crystallin, CRYAB, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, HspB5, Rosenthal fiber component

SwissProt ID

P02510 (Bovin), P02511 (Human), P23927 (Mouse), P23928 (Rat), Q05713 (Chick), Q5ENY9 (Sheep), Q7M2W6 (Pig)

Gene ID

1410 (CRYAB), 12955 (Cryab)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Hst (Hamster), Por (Porcine), Rb (Rabbit), Chk (Chicken), Can (Canine), GP (Guinea Pig), Mky (Monkey), Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)

Background of Alpha-crystallin B chain / CRYA2 antibody

Alpha-crystallins composed of ~20 kDa alphaA and alphaB subunits function as major water-soluble proteins accounting for almost 50% of total protein in the mammalian transparent eye lens, also existing in a variety of other tissues1. Crystallin families beta and gamma share homology with each other but not the alpha-crystallin family or the small heat shock protein (sHsp) family. sHsps including the alpha- crystallin proteins are induced by heat and other stress insults in a variety of organisms. The alpha-crystallins possess structural and functional similarities and share sequence homology with Hsp25/273. Most sHsps exhibit four common structural and functional features: monomeric molecular weight between 12 and 43kDa; the formation of large oligomeric complexes especially for alphaA-crystallin, alphaB-crystallin and Hsp25/27; a moderately conserved alpha-crystallin domain in the central region of the protein; and molecular chaperone activity. The alpha-crystallin domain bounded by variable N-terminal and C-terminal extensions contains approximately 80 residues and participates in oligomer assembly. Oligomers, potentially 800kDa or more, exhibit dynamic subunit exchanges and organizational plasticity, which may promote functional diversity. Phosphorylation of serine residues specifically for Hsp27 occurs in response to stress during development, typically decreasing oligomer size. Chaperone activity requires oligomerization (which, in turn, modulates the chaperone activity) and is confined to binding unfolded intermediates to prevent irreversible aggregation, even though productive release and refolding of denatured proteins requires close cooperation with other chaperones. Other proposed functions include a role in membrane stabilization and modulation of intermediate filament organization during physiological stress and neurodegenerative disease.

General readings

This product has been used in:Golenhofen N et al. Expression and induction of the stress protein alpha-B-crystallin in vascular endothelial cells. Histochem Cell Biol 117:203-9 (2002). PubMed PMID: 11914917
Harris MB & Starnes JW Effects of body temperature during exercise training on myocardial adaptations. Am J Physiol Heart Circ Physiol 280:H2271-80 (2001). PubMed PMID: 11299231
Yang J et al. Decreased SLIM1 expression and increased gelsolin expression in failing human hearts measured by high-density oligonucleotide arrays. Circulation 102:3046-52 (2000). PubMed PMID: 11120693
Suzuki A et al. MKBP, a novel member of the small heat shock protein family, binds and activates the myotonic dystrophy protein kinase. J Cell Biol 140:1113-24 (1998). PubMed PMID: 9490724

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500583) Mouse IgG1 OTI6D11 Purified Hu, Mky, Rt F, ICC/IF, IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

NULL Mouse IgG1 3A10.C9 Aff - Purified Bov, Hu E, P, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Alpha-crystallin B chain / CRYA2 antibody

NULL Mouse IgG1 3A10.C9 Aff - Purified Bov, Hu E, P, WB
50 µg / €220.00
  Acris Antibodies GmbH

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500550) Mouse IgG1 OTI2A1 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500573) Mouse IgG1 OTI3c2 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500581) Mouse IgG1 OTI6A9 Purified Hu, Rt F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500582) Mouse IgG1 OTI4A6 Purified Hu F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500584) Mouse IgG1 OTI5C9 Purified Hu ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Anti-CRYAB mouse monoclonal antibody (TA500590) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB(RC202718). Mouse IgG1 OTI1G1 Purified Hu F, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500598) Mouse IgG1 OTI4G5 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500599) Mouse IgG2b OTI6G5 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemistry-Paraffin: Crystallin AB Antibody (10D5F4/1D11C6E6) [NBP1-47519] - Immunohistochemical analysis of paraffin-embedded human brain hippocampus (A), lung cancer (B), brain tumor (C), breast cance (D), showing cytoplasmic localization with DAB staining using anti-CRYAB mAb. Mouse IgG2b 10D5F4/1D11C6E6 Ascites Hu C, E, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemistry-Paraffin: Crystallin AB Antibody (6A9) [NBP1-47708] - Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-CRYABmouse monoclonal antibody. (Dilution 1:50) Mouse 6A9 Aff - Purified Hu, Rt F, ICC/IF, IP, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

Alpha-crystallin B chain / CRYA2 antibody

Predicted band size: 20kDa. Positive control: Mouse heart and human chromaffin cell tumor tissue, mouse muscle tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Mouse heart tissue lysate Lane2: Human chromaffin cell tumor tissue lysate Lane3: Mouse muscle tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-crystallin B chain / CRYA2 antibody

Predicted band size: 20kDa. Positive control: Mouse heart and human chromaffin cell tumor tissue, mouse muscle tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Mouse heart tissue lysate Lane2: Human chromaffin cell tumor tissue lysate Lane3: Mouse muscle tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-crystallin B chain / CRYA2 antibody

Western blot analysis of AlphaB Crystallin in bovine cell lines using a 1:1000 dilution of the antibody Mouse IgG1 1A7.D5 Aff - Purified Bov, Hu E, P, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Alpha-crystallin B chain / CRYA2 antibody

Alpha-crystallin B chain / CRYA2 Rabbit IgG Serum Bov, Chk, Hu, Ms, Rt C, ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Alpha-crystallin B chain / CRYA2 antibody

Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody (TA500580). Mouse IgG1 OTI3E3 Purified Hu ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Anti-CRYAB mouse monoclonal antibody (TA500680) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB(RC202718). Mouse IgG1 OTI5D8 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-crystallin B chain / CRYA2 antibody

Immunohistochemical analysis of paraffin-embedded human brain hippocampus (A), lung cancer (B), brain tumor (C), breast cance (D), showing cytoplasmic localization with DAB staining using CRYAB antibody Cat.-No AM06212SU-N Mouse IgG2a 1D11C6E6 Ascites Hu E, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

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