Alpha-enolase / ENO1 antibody

Principal name

Alpha-enolase / ENO1 antibody

Alternative names for Alpha-enolase / ENO1 antibody

Enolase 1, ENO1L1 Non-neural enolase, NNE, Plasminogen-binding protein, MBP-1, MPB-1, MBPB1, MPB1, 2-phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase, C-myc promoter-binding protein

SwissProt ID

P04764 (Rat), P06733 (Human), P17182 (Mouse), P51913 (Chick), Q9XSJ4 (Bovin)

Gene ID

2023 (ENO1), 24333 (Eno1)

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine), African clawed frog, Arabidopsis, Ce (C. elegans), Ze (Zebrafish), Chimp (Chimpanzee), Mky (Monkey), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep), Eq (Equine), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Dot blot (Dot), Flow Cytometry (F)

Background of Alpha-enolase / ENO1 antibody

Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. The Enzyme Commission classification number for this class of enzyme is EC4.2.1.11, and they can also be referred to as 2-phospho-D-glycerate hydrolases. There are three enolase proteins each coded for by a distinct gene. They are known as Enolase 1, 2 and 3 or altertely as alpha, beta and gamma-Enolase. Confusingly, although Enolase 1 is alpha-Enolase, Enolase 2 corresponds to gamma-Enolase while Enolase 3 is also known as beta-Enolase. All three enzymes exist as dimers in vivo and the three molecules are very similar in primary amino acids sequence. This similarity allows not only homodimers but also most possible heterodimers to form. The expression pattern of the three enzymes differs in a tissue specific manner, so that antibodies to them are useful cell type specific markers, especially as all three molecules are very abundant. Enolase 1 or alpha is also known as non-neurol Enolase (NNE) and is expressed in most kinds of tissue, but is absent from neurons. Abnormal expression of NNE is associated with tumor progression in some breast and head and neck cancer. Enolase 2 or gamma is also known as neuron specific enolase (NSE). A switch from NNE to NSE occurs in the development of neurons. Enolase 3 or beta is expressed primarily in muscle cells.

General readings

Ghosh,A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-enolase / ENO1 antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308873 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 (1-435) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 8G8 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

ENO1 polyclonal antibody (A01), Lot # SFU4050831QCS1. Western Blot analysis of ENO1 expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Alpha-enolase / ENO1 antibody

Immunoperoxidase of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1ug/ml] Mouse IgG1 8G8 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-enolase / ENO1 antibody

ENO1 Antibody (Cat. #TA324640) western blot analysis in MCF-7,A431,Hela,HepG2,mouse NIH/3T3 cell line and mouse stomach,liver tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow). Mouse IgG 786CT6.6.4 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

ENOA Antibody (N-term) (Cat. #TA324751) western blot analysis in U251,mouse C2C12,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow). Rabbit IgG Purified Bov, Hu, Mky, Ms F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

ENO1 Antibody (Center) (Cat. #TA324847) western blot analysis in Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow). Rabbit Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

Western blot analysis of extracts of various cell lines, using ENO1 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

WB Suggested Anti-ENO1 Antibody Titration: 0.5ug/ml; Positive Control: Jurkat cell lysateENO1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

WB Suggested Anti-ENO1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysateENO1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified GP, Hu, Ms, Rb, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

WB Suggested Anti-ENO1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate. ENO1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Eq, GP, Hu, Rb, Rt, Sh P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

WB Suggested Anti-ENO1 Antibody Titration: 0.03 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Small Intestine Rabbit IgG Purified Can, GP, Hu, Por, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

Simple Western: Enolase 1 Antibody (253) [TA337100] - Simple Western lane view shows a specific band detected for Enolase 1 in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or Mouse IgG1 253 Purified Bov, Hu, Ms, Rt, Xen ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

Alpha-enolase / ENO1 Mouse IgG1 B823M Purified Hu E, WB
1 mg / €550.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

Alpha-enolase / ENO1 Mouse IgG1 NSE-P1 Purified Hu C, E, P, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 (N-term) antibody

Human Colon (formalin-fixed, paraffin-embedded) stained with ENO1 antibody AP22804PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Ig Fraction Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

Human Breast (formalin-fixed, paraffin-embedded) stained with ENO1 antibody AP22805PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Ig Fraction Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

Staining of Jurkat cells with Rabbit anti Human NNE Antibody Cat.-No BP087. Rabbit Purified Hu C, E, P, WB
1 ml / €360.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

Western Blot analysis of ENO1 expression in transfected 293T cell line (H00002023-T01) by ENO1 MaxPab polyclonal antibody.

Lane 1: ENO1 transfected lysate(47.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Alpha-enolase / ENO1 antibody

ENO1 monoclonal antibody (M03), clone 3C8. Western Blot analysis of ENO1 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG1 3C8 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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