Alpha-enolase / ENO1 antibody

Principal name

Alpha-enolase / ENO1 antibody

Alternative names for Alpha-enolase / ENO1 antibody

Enolase 1, ENO1L1 Non-neural enolase, NNE, Plasminogen-binding protein, MBP-1, MPB-1, MBPB1, MPB1, 2-phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase, C-myc promoter-binding protein

SwissProt ID

P04764 (Rat), P06733 (Human), P17182 (Mouse), P51913 (Chick), Q9XSJ4 (Bovin)

Gene ID

2023 (ENO1), 24333 (Eno1), 433182 (Gm5506), 13806

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Por (Porcine), Arabidopsis, Ce (C. elegans), Ze (Zebrafish), Mky (Monkey), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep), Eq (Equine), Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Alpha-enolase / ENO1 antibody

Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. The Enzyme Commission classification number for this class of enzyme is EC4.2.1.11, and they can also be referred to as 2-phospho-D-glycerate hydrolases. There are three enolase proteins each coded for by a distinct gene. They are known as Enolase 1, 2 and 3 or altertely as alpha, beta and gamma-Enolase. Confusingly, although Enolase 1 is alpha-Enolase, Enolase 2 corresponds to gamma-Enolase while Enolase 3 is also known as beta-Enolase. All three enzymes exist as dimers in vivo and the three molecules are very similar in primary amino acids sequence. This similarity allows not only homodimers but also most possible heterodimers to form. The expression pattern of the three enzymes differs in a tissue specific manner, so that antibodies to them are useful cell type specific markers, especially as all three molecules are very abundant. Enolase 1 or alpha is also known as non-neurol Enolase (NNE) and is expressed in most kinds of tissue, but is absent from neurons. Abnormal expression of NNE is associated with tumor progression in some breast and head and neck cancer. Enolase 2 or gamma is also known as neuron specific enolase (NSE). A switch from NNE to NSE occurs in the development of neurons. Enolase 3 or beta is expressed primarily in muscle cells.

General readings

Ghosh,A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-enolase / ENO1 antibody

  Rabbit Polyclonal antibody to ENO1 (enolase 1, (alpha))  
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308873 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 (1-435) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 8G8 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 (178-205) antibody

Human Breast (formalin-fixed, paraffin-embedded) stained with ENO1 antibody AP22805PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Ig Fraction Hu F, ICC/IF, P, WB
0.2 ml / €470.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

  Mouse monoclonal ENO1 Antibody  
ENO1 Antibody (Cat. #TA324640) western blot analysis in MCF-7,A431,Hela,HepG2,mouse NIH/3T3 cell line and mouse stomach,liver tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow). Mouse IgG 786CT6.6.4 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Rabbit polyclonal ENOA Antibody (N-term)  
ENOA Antibody (N-term) (Cat. #TA324751) western blot analysis in U251,mouse C2C12,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow). Rabbit IgG Purified Bov, Hu, Mky, Ms F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Rabbit polyclonal ENO1 Antibody (Center)  
ENO1 Antibody (Center) (Cat. #TA324847) western blot analysis in Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow). Rabbit Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Rabbit anti-ENO1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ENO1 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Rabbit Polyclonal Anti-ENO1 Antibody  
WB Suggested Anti-ENO1 Antibody Titration: 0.5ug/ml; Positive Control: Jurkat cell lysateENO1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Rabbit Polyclonal Anti-ENO1 Antibody  
WB Suggested Anti-ENO1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysateENO1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified GP, Hu, Ms, Rb, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Rabbit Polyclonal Anti-ENO1 Antibody  
WB Suggested Anti-ENO1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate. ENO1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Eq, GP, Hu, Rb, Rt, Sh P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Rabbit Polyclonal Anti-ENO1 Antibody  
WB Suggested Anti-ENO1 Antibody Titration: 0.03 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Small Intestine Rabbit IgG Purified Can, GP, Hu, Por, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Mouse Monoclonal Enolase 1 Antibody (253)  
Simple Western: Enolase 1 Antibody (253) [TA337100] - Simple Western lane view shows a specific band detected for Enolase 1 in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or Mouse IgG1 253 Purified Bov, Hu, Ms, Rt, Xen ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

  Rabbit Polyclonal Anti-ENO1 Antibody  
Alpha-enolase / ENO1 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-enolase / ENO1 antibody

Alpha-enolase / ENO1 Mouse IgG1 B823M Purified Hu E, WB
1 mg / €590.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

Alpha-enolase / ENO1 Mouse IgG1 NSE-P1 Purified Hu C, E, P, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 (N-term) antibody

Human Colon (formalin-fixed, paraffin-embedded) stained with ENO1 antibody AP22804PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Ig Fraction Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

Staining of Jurkat cells with Rabbit anti Human NNE Antibody Cat.-No BP087. Rabbit Purified Hu C, E, P, WB
1 ml / €360.00
  Acris Antibodies GmbH

Alpha-enolase / ENO1 antibody

Alpha-enolase / ENO1 Mouse IgG1 H11 Purified E, WB
1 mg / €430.00
  HyTest Ltd.

CRISPR

Catalog No. Species Donor Vector  

Eno1b - mouse gene knockout kit via CRISPR

Eno1b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Eno1b - mouse gene knockout kit via CRISPR

Eno1b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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