Alpha-fetoprotein / AFP antibody

Principal name

Alpha-fetoprotein / AFP antibody

Alternative names for Alpha-fetoprotein / AFP antibody

Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

SwissProt ID

P02771 (Human), P02772 (Mouse), P02773 (Rat), Q3SZ57 (Bovin), Q8MJ76 (Pig), Q8MJU5 (Canfa)

Gene ID

174 (AFP), 11576 (Afp)

Available reactivities

Hu (Human), Bov (Bovine), Mky (Monkey), Can (Canine), Por (Porcine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Radioimmunoassay (R), Frozen Sections (C), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA capture and detection (E(caption)/E(detection)), Lateral Flow (LF), EPair for Elisa (EPair), Immunodiffusion (ID), Flow Cytometry (F)

Background of Alpha-fetoprotein / AFP antibody

Alpha-fetoprotein (AFP) is a major plasma protein that is synthesized primarily in the fetal liver and gastrointestinal tract and embryonic yolk sac (1). AFP is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP production is essentially stopped soon after birth; however, it starts up again under the stimulus of some diseases in the liver. It may be produced by the liver in viral hepatitis and cirrhosis of the liver (2). AFP is also made by primary liver tumors (hepatomas) and by germ cell tumors (teratocarcinoma and embryonal cell carcinomas) hence AFP expression in adults is often associated with hepatoma or tetratoma (2-4). A person’s serum AFP level can therefore be used to help detect these conditions and monitor their treatment. Hereditary persistence of AFP may also be found in individuals with no obvious pathology. The level of AFP in amniotic fluid is used to measure renal loss of AFP protein to screen for spina bifida and anencephaly in pregnant mothers (5, 6).
AFP can be found in the amniotic fluid and in the mother’s blood. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin that serves as the basis for some valuable tests. A problem with the fetus, placenta, a tumor or liver disease in a pregnant woman could result in abnormally high levels of AFP in the serum of that pregnant woman. Abnormally elevated level of AFP in amniotic fluid could be due to open neural tube defect, open abdominal wall defect, congenital nephrosis and other causes. Alpha-fetoprotein (AFP) is approximately 70 kDa (609 Amino Acids) protein.

General readings

Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I.: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes.
J Immunol Methods. 1988 Jun 28;111(1):67-73.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-fetoprotein / AFP antibody

  AFP mouse monoclonal antibody, clone UMAB66  
Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-AFP mouse monoclonal antibody. (UM500056; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB66 Purified Hu ICC/IF, P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Alpha-fetoprotein / AFP antibody

  AFP mouse monoclonal antibody, clone UMAB66  
Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-AFP mouse monoclonal antibody. (UM500056; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB66 Purified Hu ICC/IF, P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Alpha-fetoprotein / AFP antibody

  Rabbit Monoclonal anti-Alpha-Fetoprotein (AFP) Antibody  
Western Blot analysis of HepG2 cell lysate with AFP antibody. Rabbit IgG SP154 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-fetoprotein / AFP antibody

  AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501782) Mouse IgG2b OTI2A9 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-fetoprotein / AFP antibody

  anti-AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501783, Dilution 1:50) Mouse IgG1 OTI1E8 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-fetoprotein / AFP antibody

  AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)  
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AFP mouse monoclonal antibody. (TA501785) Mouse IgG1 OTI1D1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-fetoprotein / AFP antibody

  anti-AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI2E2 (formerly 2E2)  
Anti-AFP mouse monoclonal antibody (TA501788) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP(RC206622). Mouse IgG2a OTI2E2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-fetoprotein / AFP antibody

  AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI3G3 (formerly 3G3)  
Anti-AFP mouse monoclonal antibody (TA501789) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP(RC206622). Mouse IgG1 OTI3G3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-fetoprotein / AFP antibody

  Rabbit Polyclonal Alpha 1 Fetoprotein Antibody  
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-AFP rabbit polyclonal antibody. (TA590065) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-fetoprotein / AFP antibody

  Rabbit Polyclonal Alpha 1 Fetoprotein Antibody  
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-AFP rabbit polyclonal antibody. (TA590066) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-fetoprotein / AFP antibody

  AFP mouse monoclonal antibody, clone OTI2F11 (formerly 2F11)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AFP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809081)(1:150) Mouse IgG2b OTI2F11 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-fetoprotein / AFP antibody

  AFP mouse monoclonal antibody, clone OTI4G9 (formerly 4G9)  
Anti-AFP mouse monoclonal antibody (TA809082) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP(RC206622).(1:100) Mouse IgG2a OTI4G9 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-fetoprotein / AFP antibody

  AFP mouse monoclonal antibody, clone OTI4D8 (formerly 4D8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AFP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809083)(1:150) Mouse IgG2a OTI4D8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-fetoprotein / AFP antibody

Formalin-Paraffin Human fetal liver stained with AFP Antibody Cat.-No AM33373PU (Clone C3). Mouse IgG2a C3 Purified Can, Hu, Mky, Por C, F, ICC/IF, IP, P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

Alpha-fetoprotein / AFP antibody

Formalin-Paraffin Human fetal liver stained with AFP Antibody Cat.-No AM33373PU (Clone C3). Mouse IgG2a C3 Purified Can, Hu, Mky, Por C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Alpha-fetoprotein / AFP antibody

Formalin-Paraffin Human fetal liver stained with AFP Antibody Cat.-No AM33373PU (Clone C3). Mouse IgG2a C3 Purified Can, Hu, Mky, Por C, F, ICC/IF, IP, P, WB
20 µg / €180.00
  Acris Antibodies GmbH

Alpha-fetoprotein / AFP antibody

Formalin-Fixed, Paraffin-Embedded Human fetal liver stained with AFP Antibody Cat.-No AM50105PU (Clone SPM334). Mouse IgG2a SPM334 Purified Can, Hu, Mky, Por C, F, ICC/IF, IP, P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

Alpha-fetoprotein / AFP antibody

Formalin-Fixed, Paraffin-Embedded Human fetal liver stained with AFP Antibody Cat.-No AM50105PU (Clone SPM334). Mouse IgG2a SPM334 Purified Can, Hu, Mky, Por C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Alpha-fetoprotein / AFP antibody

Formalin-Fixed, Paraffin-Embedded Human fetal liver stained with AFP Antibody Cat.-No AM50105PU (Clone SPM334). Mouse IgG2a SPM334 Purified Can, Hu, Mky, Por C, F, ICC/IF, IP, P, WB
20 µg / €180.00
  Acris Antibodies GmbH

Alpha-fetoprotein / AFP antibody

  Rabbit Polyclonal Antibody against AFP (Center)  
Western blot analysis of AFP (arrow) using rabbit polyclonal AFP  Antibody (Center) (Cat.#TA302117). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AFP  gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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