alpha skeletal muscle Actin / ACTA1 antibody

Principal name

alpha skeletal muscle Actin / ACTA1 antibody

SwissProt ID

P68133 (Human), P68134 (Mouse), P68135 (Rabit), P68136 (Rat), P68137 (Pig), P68138 (Bovin), P68139 (Chick)

Gene ID

58 (ACTA1), 421534

Available reactivities

Hu (Human), Bov (Bovine), GP (Guinea Pig), Gt (Goat), Mky (Monkey), Ms (Mouse), Por (Porcine), Rt (Rat), Sh (Sheep), Chk (Chicken), Can (Canine), Sw (Silk Worm), Carp, Rb (Rabbit), Snake, Xen (Xenopus), Frog, Fi (Fish), Ze (Zebrafish), All Species

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunoprecipitation (IP)

Background of alpha skeletal muscle Actin / ACTA1 antibody

Actin is one of the most abundant and highly conserved proteins of eukaryotes. Mammalian Actins are the product of six different genes with differing distribution patterns in cell types and in tissues. The molecular weight of all six proteins is 42 kDa, and one or more actins are found in essentially every type of crude cellular and tissue extract. As a result, antibodies to Actin are widely used. As western blotting standards, they can be used to verify that the various steps of the western blotting procedure have been performed correctly. In addition, Actin is regarded as a "housekeeping" protein which is generally not altered much in expression as a result of experimental manipulations. Thus, quantitation of the Actin band on the western is used as a standard against the band density of other proteins. The Actin isotypes were origilly classified as alpha, beta and gamma forms based on their charges which resulted in different mobilities in the isoelectric focusing dimension as seen on 2-dimensiol SDS-PAGE. Subsequently, the alpha spot was found to potentially contain three Actin gene products, alpha-skeletal Actin, alpha-vascular smooth muscle Actin, and alpha-cardiac muscle. The beta spot contained a single protein called simply beta Actin, while the gamma spot may contain both gamma-1 Actin and gamma-2, which are enteric and smooth muscle Actins. The six mammalian gene products are very similar as shown by a sequence alignment of the human proteins. The most closely related actin isotypes are 97% identical, while the most distantly related are 94% identical, with most of the variability at the N-terminus. With this level of identity, antibodies to any one protein may recognize all six isotypes, as is the case with clone 5J11. This antibody binds all six isotypes very strongly on western blots, and it is a very effective blotting standard which can work on any cell type or tissue extract. It also works in immunocytochemical experiments, binding strongly and cleanly to filopodia, membrane ruffles and stress fibers, all known to be rich in Actin.

General readings

General / background references:Skalli O et al. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas. Am J Pathol 130:515-31 (1988). PubMed PMID: 3279794
Babai F et al. Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms. Virchows Arch B Cell Pathol Incl Mol Pathol 55:263-77 (1988). PubMed PMID: 2901166
Schurch W et al. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors. Am J Pathol 128:91-103 (1987). PubMed PMID: 2440309

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Actin (pan) antibody

Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein. Mouse IgG1 C4 Ascites All Species C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin (Muscle Specific) antibody

Formalin-Paraffin leiomyosarcoma (10X) stained with muscle specific Actin Antibody Cat.-No AM33291PU (Clone SPM160). Mouse IgG1 SPM160 Purified Hu, Rb, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Actin (Muscle Specific) antibody

Formalin-Paraffin leiomyosarcoma (10X) stained with muscle specific Actin Antibody Cat.-No AM33291PU (Clone SPM160). Mouse IgG1 SPM160 Purified Hu, Rb, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Actin (Muscle Specific) antibody

Formalin-Paraffin leiomyosarcoma (10X) stained with muscle specific Actin Antibody Cat.-No AM33291PU (Clone SPM160). Mouse IgG1 SPM160 Purified Hu, Rb, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Actin antibody

Actin Rabbit IgG Q20-K FITC Hu F
100 Tests / €540.00
  DB Biotech, spol. s r.o.

Sample sizes available

alpha skeletal muscle Actin / ACTA1 antibody

Western Blot: Actin Antibody (mAbGEa) [TA336491] - Western blot analysis of Actin expression in 2) HeLa, 3) NTERA-2, 4) A431, 5) HepG2, 6) MCF7, 7) NIH-3T3, 8) PC-12 and 9) COS-7 whole cell lysates. Mouse IgM mAbGEa Purified Bov, Hu, Ms, Rt C, E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha skeletal muscle Actin / ACTA1 (1-129) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Fi, Hu, Ze P, WB
50 µl / €470.00
  Acris Antibodies GmbH

alpha skeletal muscle Actin / ACTA1 (N-term) antibody

Human skeletal muscle stained with anti-actin antibody Mouse IgM 5C5.F8.C7 Purified Hu P
0.5 ml / €310.00
  Acris Antibodies GmbH

alpha skeletal muscle Actin / ACTA1 (N-term) antibody

Human skeletal muscle stained with anti-actin antibody Mouse IgM 5C5.F8.C7 Purified Hu P
1 ml / €440.00
  Acris Antibodies GmbH

alpha skeletal muscle Actin / ACTA1 (N-term) antibody

Human skeletal muscle stained with anti-actin antibody Mouse IgM 5C5.F8.C7 Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

alpha skeletal muscle Actin / ACTA1 antibody

Skeletal muscle stained with Anti-Actin, skeletal muscle antibody (Cat#AP15334PU) Rabbit Purified Bov, Frog, GP, Hu, Rb, Rt, Sh P
0.5 ml / €300.00
  Acris Antibodies GmbH

alpha skeletal muscle Actin / ACTA1 antibody

Skeletal muscle stained with Anti-Actin, skeletal muscle antibody (Cat#AP15334PU) Rabbit Purified Bov, Frog, GP, Hu, Rb, Rt, Sh P
1 ml / €330.00
  Acris Antibodies GmbH

alpha skeletal muscle Actin / ACTA1 antibody

Skeletal muscle stained with Anti-Actin, skeletal muscle antibody (Cat#AP15334PU) Rabbit Purified Bov, Frog, GP, Hu, Rb, Rt, Sh P
0.1 ml / €170.00
  Acris Antibodies GmbH

alpha skeletal muscle Actin / ACTA1 antibody

Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. C: BCL-1. D: C2C12. 10% SDS PAGE. TA308837 diluted at 1:5000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha skeletal muscle Actin / ACTA1 antibody

Sample (30 ug of whole cell lysate). A: Raji. 10% SDS PAGE. TA308838 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha skeletal muscle Actin / ACTA1 antibody

Western blot analysis of extracts from LOVO cells, using Actin-a-1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha skeletal muscle Actin / ACTA1 antibody

Predicted band size: 42kDa. Positive control: Mouse muscle and heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Mouse muscle tissue lysate Lane2: Mouse heart tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha skeletal muscle Actin / ACTA1 (C-term) antibody

ACTA1/α-actin Antibody (C-term) (Cat.# TA324566) western blot analysis in A549,RD cell line and human placenta lysates (35ug/lane).This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow). Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

alpha skeletal muscle Actin / ACTA1 antibody

Western Blot: Actin Antibody (5J11) [TA337095] - Crude extracts of HeLa. Lane 15 was probed with this Actin antibody TA337095, giving a strong clean band at 42kDa. Lane 16 was probed with NBP1-92691, the 14-3-3 eta antibody, which has a molecular weight o Mouse IgG1 5J11 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha skeletal muscle Actin / ACTA1 antibody

Paraffin sections of human striated skeletal muscle from human tongue immunostained with 3B3 (1/250) Mouse IgG1 3B3 Purified Hu, Por, Rb, Rt C, E, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

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