Alpha-Synuclein / SNCA antibody

Principal name

Alpha-Synuclein / SNCA antibody

Alternative names for Alpha-Synuclein / SNCA antibody

NACP, PARK1, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

SwissProt ID

O55042 (Mouse), P37377 (Rat), P37840 (Human), Q3T0G8 (Bovin)

Gene ID

6622 (SNCA), 29219 (Snca), 20617

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Chk (Chicken), Sh (Sheep), Rb (Rabbit), Mam (Mammalia)

Available hosts

Mouse, Rabbit, Goat, Sheep, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Radioimmunoassay (R), EPair for Elisa (EPair), Dot blot (Dot), Immunoelectron Microscopy (EM)

Background of Alpha-Synuclein / SNCA antibody

Alpha-synuclein is expressed principally in the central nervous system (brain) but is also expressed in low concentrations in a variety of tissues except liver. It is predominantly expressed in the neocortex, hippocampus, substantia nigra, thalamus, and cerebellum of the CNS. It is primarily a neuronal protein, but can also be found in the neuroglial cells. It is concentrated in presynaptic nerve terminals of neurons, as well as having reported nuclear and mitochondrial localization. Alpha-synuclein interacts with plasma membrane phospholipids. Alpha-synuclein in solution is considered to be an intrinsically disordered protein and thus lacks a stable secondary or tertiary structure. However, data suggests the presence of partial alpha helical as well as beta sheet structures as well as mostly structured tetrameric states in solution, the equilibrium of which may be altered by binding partners. The human alpha-synuclein protein is made of 140 amino acids, encoded by the SNCA gene. The primary structure is divided in three distinct domains: (1-60) - An amphipathic N-terminal region dominated by four 11-residue repeats including the consensus sequence KTKEGV. This sequence has a structural alpha helix propensity similar to apolipoproteins-binding domains. (61-95) - a central hydrophobic region which includes the non-amyloid- beta; component (NAC) region, involved in protein aggregation. (96-140) - a highly acidic and proline-rich region. At least three isoforms of synuclein are produced through alternative splicing. The most common form of the protein, is the full 140 amino acid-long transcript. Other isoforms are alpha-synuclein-126, lacking residues 41-54; and alpha-synuclein-112, which lacks residues 103-130. a-synuclein may be involved in the regulation of dopamine release and transport and also may function to induce fibrillization of microtubule-associated protein tau. alpha-synuclein functions as a molecular chaperone in the formation of SNARE complexes. In particular, it can bind to phospholipids of the plasma membrane and to synaptobrevin-2 via its C-terminus domain to influence synaptic activity. alpha-synuclein is essential for normal development of the cognitive functions and that it significantly interacts with tubulin. It also reduces neuronal responsiveness to various apoptotic stimuli, leading to decreased caspase-3 activation. Alpha-synuclein fibrils are major substituent of the intracellular Lewy bodies seen in Parkinson rsquo;s disease. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.

General readings

1. Jakes, R., et al. (1994) FEBS lett. 345:27-32.
2. Ueda, K., et al. (1993) Proc. Natl.Acad. Sci. USA 90:11282-11286.
3. Kim, J. (1997) Molecules and Cells 7:78-83.
4. Paik, S. R., et al. (1997) Arch. Biochem. Biophys. 344:325-334.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-Synuclein / SNCA (also beta reactive) antibody

Flow cytometry analysis of α,β -Synuclein LNCaP cell line staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG mouse IgG Alexa fluor 488 conjugate Isotype control antibody was mouse IgG (black line). Mouse IgG1 3B6 Purified Hu, Ms, Rt E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA (also beta reactive) antibody

Flow cytometry analysis of α,β -Synuclein LNCaP cell line staining at 2-5ug for 1x106 cells (red line). The secondary antibody used goat anti mouse IgG mouse IgG Alexa fluor 488 conjugate Isotype control antibody was mouse IgG (black line). Mouse IgG1 3B6 Purified Hu, Ms, Rt E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

  Rabbit polyclonal a-Synuclein (Ab-133) antibody  
Western blot analysis of extracts from 293 cells, treated with Etoposide 25µM 60', using Synuclein-alpha (Ab-133) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

  Rabbit polyclonal Synuclein-pan antibody  
Western blot analysis of extracts from rat heart, using Synuclein-pan antibody (#TA311770).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-Synuclein / SNCA antibody

Alpha-Synuclein / SNCA Mouse IgG1 4D6 Purified Hu C, E, P, WB
0.2 ml / €1,120.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

Extracts of SHSY-5Y human neuroblastoma cells were resolved by SDS PAGE and transferred to a PVDF membrane. Membranes were incubated with 1 μg/mL of the anti-α synuclein antibody (Syn 211). After washing, membranes were incubated with goat F(ab’)2 anti Mouse IgG alkaline phosphatase diluted 1:5000 and the membrane was incubated with CDP-Substrate using the WesternStarTM method (Tropix). The membrane was then exposed to Kodak BioMax film. Mouse IgG1 Syn 211 Purified Hu C, E, WB
0.1 mg / €610.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

Immunohistochemical analysis of paraffin-embedded human glioma tissue, showing membrane localization using SNCA antibody Cat.-No AM06128SU-N with DAB staining. Mouse IgG 2B2D1 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

The recombinant human synuclein family (alpha-, beta- and gamma-) proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha-synuclein antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1E10 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

The recombinant human synuclein family (alpha-, beta- and gamma-) proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha-synuclein antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1E10 Purified Hu E, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA pSer129 antibody

Immunohistochemistry (IHC) analysis of SNCA antibody (Cat.-No.: AP01870PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €380.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

Immunohistochemistry (IHC) analyzes of SNCA antibody (Cat.-No.: AP06582PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu E, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA antibody

Alpha-Synuclein / SNCA Rabbit Purified Hu, Rt C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA pSer129 antibody

Alpha-Synuclein / SNCA Rabbit Purified Hu, Rt C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA pSer87 antibody

Alpha-Synuclein / SNCA Rabbit Purified Hu, Ms, Rt C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA (61-95) antibody

Staining of Formalin-Fixed, Paraffin-Embedded Human Brain using  SNCA antibody Cat.-No. SM6028 at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG1 5C2 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA (61-95) antibody

Staining of Formalin-Fixed, Paraffin-Embedded Human Brain using  SNCA antibody Cat.-No. SM6028 at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG1 5C2 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Alpha-Synuclein / SNCA pSer129 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Synuclein (Phospho-Ser129) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

Alpha-Synuclein / SNCA pSer129 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Synuclein (Phospho-Ser129) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

Alpha-Synuclein / SNCA pTyr133 antibody

Western blot analysis of extracts from 293 cells treated with Etoposide 25 muM 60', using Synuclein-alpha (Phospho-Tyr133) Antibody. The lane on the left is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

Items 1 to 20 of 33 total

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