alpha Tubulin / TUBA1B antibody

Principal name

alpha Tubulin / TUBA1B antibody

Alternative names for alpha Tubulin / TUBA1B antibody

Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1

SwissProt ID

P05213 (Mouse), P68361 (Crigr), P68363 (Human), P81947 (Bovin), Q2XVP4 (Pig), Q6P9V9 (Rat)

Gene ID

10376 (TUBA1B)

Available reactivities

All Species, Av (Birds), Dros (Drosophila), Fu (Fungi), Hu (Human), Mam (Mammalia), Ms (Mouse), Rt (Rat), Ye (Yeast), Por (Porcine), Bov (Bovine), Hst (Hamster), Chk (Chicken), Mky (Monkey), Frog, GP (Guinea Pig), Ge (Gerbil), Ze (Zebrafish), Chimp (Chimpanzee)

Available hosts

Mouse, Rat, Rabbit

Available applications

Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Radioimmunoassay (R), ELISA (detection) (E(detection)), ChIP assay (ChIP)

Background of alpha Tubulin / TUBA1B antibody

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.

The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.
Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.

General readings

1. Kilmartin JV, Wright B, Milstein C. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J Cell Biol. 1982 Jun;93(3):576-82. PubMed PMID: 6811596.
2. Wehland J, Willingham MC, Sandoval IV. A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo. J Cell Biol. 1983 Nov;97(5 Pt 1):1467-75. PubMed PMID: 6415068.
3. Wallace SW, Durgan J, Jin D, Hall A. Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B. Mol Biol Cell. 2010 Sep 1;21(17):2996-3006. doi: 10.1091/mbc.E10-05-0429. Epub 2010 Jul 14. PubMed PMID: 20631255.
4. Wehland J, Schröder HC, Weber K. Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2. EMBO J. 1984 Jun;3(6):1295-300. PubMed PMID: 6204858.
5. Burns R. Cytoskeleton. Tubulin's terminal tyrosine. Nature. 1987 May 14-20;327(6118):103-4. PubMed PMID: 3574472.
6. Skinner RH, Bradley S, Brown AL, Johnson NJ, Rhodes S, Stammers DK, et al. Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating proteins. J Biol Chem. 1991 Aug 5;266(22):14163-6. PubMed PMID: 1713577.
7. Abe Y, Okumura E, Hosoya T, Hirota T, Kishimoto T. A single starfish Aurora kinase performs the combined functions of Aurora-A and Aurora-B in human cells. J Cell Sci. 2010 Nov 15;123(Pt 22):3978-88. doi: 10.1242/jcs.076315. PubMed PMID: 21048162.
8. Cheishvili D, Maayan C, Cohen-Kupiec R, Lefler S, Weil M, Ast G, et al. IKAP/Elp1 involvement in cytoskeleton regulation and implication for familial dysautonomia. Hum Mol Genet. 2011 Apr 15;20(8):1585-94. doi: 10.1093/hmg/ddr036. Epub 2011 Jan 27. PubMed PMID: 21273291.
9. Berrueta L, Kraeft SK, Tirnauer JS, Schuyler SC, Chen LB, Hill DE, et al. The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules. Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10596-601. PubMed PMID: 9724749.
10. Bruce EA, Digard P, Stuart AD. The Rab11 pathway is required for influenza A virus budding and filament formation. J Virol. 2010 Jun;84(12):5848-59. doi: 10.1128/JVI.00307-10. Epub 2010 Mar 31. PubMed PMID: 20357086.
11. Jager M, Quéinnec E, Chiori R, Le Guyader H, Manuel M. Insights into the early evolution of SOX genes from expression analyses in a ctenophore. J Exp Zool B Mol Dev Evol. 2008 Dec 15;310(8):650-67. doi: 10.1002/jez.b.21244. PubMed PMID: 18942104.
12. Wise HM, Barbezange C, Jagger BW, Dalton RM, Gog JR, Curran MD, et al. Overlapping signals for translational regulation and packaging of influenza A virus segment 2. Nucleic Acids Res. 2011 Sep 1;39(17):7775-90. doi: 10.1093/nar/gkr487. Epub 2011 Jun 21. PubMed PMID: 21693560.
13. Zenner HL, Yoshimura S, Barr FA, Crump CM. Analysis of Rab GTPase-activating proteins indicates that Rab1a/b and Rab43 are important for herpes simplex virus 1 secondary envelopment. J Virol. 2011 Aug;85(16):8012-21. doi: 10.1128/JVI.00500-11. Epub 2011 Jun 15. PubMed PMID: 21680502.
14. Timm T, von Kries JP, Li X, Zempel H, Mandelkow E, Mandelkow EM. Microtubule affinity regulating kinase activity in living neurons was examined by a genetically encoded fluorescence resonance energy transfer/fluorescence lifetime imaging-based biosensor: inhibitors with therapeutic potential. J Biol Chem. 2011 Dec 2;286(48):41711-22. doi: 10.1074/jbc.M111.257865. Epub 2011 Oct 7. PubMed PMID: 21984823.
15. Virágh E, Gorjánácz M, Török I, Eichhorn T, Kallakuri S, Szlanka T, et al. Specific Cooperation Between Imp-α2 and Imp-β/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions. G3 (Bethesda). 2012 Jan;2(1):1-14. doi: 10.1534/g3.111.001073. Epub 2012 Jan 1. PubMed PMID: 22384376.
16. Courtois A, Schuh M, Ellenberg J, Hiiragi T. The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development. J Cell Biol. 2012 Aug 6;198(3):357-70. doi: 10.1083/jcb.201202135. Epub 2012 Jul 30. PubMed PMID: 22851319.
17. Feau S, Schoenberger SP, Altman A, Bécart S. SLAT regulates CD8+ T cell clonal expansion in a Cdc42- and NFAT1-dependent manner. J Immunol. 2013 Jan 1;190(1):174-83. doi: 10.4049/jimmunol.1201685. Epub 2012 Nov 28. PubMed PMID: 23197258.
18. Wise HM, Hutchinson EC, Jagger BW, Stuart AD, Kang ZH, Robb N, et al. Identification of a novel splice variant form of the influenza A virus M2 ion channel with an antigenically distinct ectodomain. PLoS Pathog. 2012;8(11):e1002998. doi: 10.1371/journal.ppat.1002998. Epub 2012 Nov 1. PubMed PMID: 23133386.
19. Dayraud C, Alié A, Jager M, Chang P, Le Guyader H, Manuel M, et al. Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. BMC Evol Biol. 2012 Jul 2;12:107. doi: 10.1186/1471-2148-12-107. PubMed PMID: 22747595.
20. Ligon LA, Shelly SS, Tokito M, Holzbaur EL. The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization. Mol Biol Cell. 2003 Apr;14(4):1405-17. PubMed PMID: 12686597.
21. Smertenko AP, Kaloriti D, Chang HY, Fiserova J, Opatrny Z, Hussey PJ. The C-terminal variable region specifies the dynamic properties of Arabidopsis microtubule-associated protein MAP65 isotypes. Plant Cell. 2008 Dec;20(12):3346-58. doi: 10.1105/tpc.108.063362. Epub 2008 Dec 5. PubMed PMID: 19060108.
22. Li Y, Shen Y, Cai C, Zhong C, Zhu L, Yuan M, et al. The type II Arabidopsis formin14 interacts with microtubules and microfilaments to regulate cell division. Plant Cell. 2010 Aug;22(8):2710-26. doi: 10.1105/tpc.110.075507. Epub 2010 Aug 13. PubMed PMID: 20709814.
23. Brunk K, Vernay B, Griffith E, Reynolds NL, Strutt D, Ingham PW, et al. Microcephalin coordinates mitosis in the syncytial Drosophila embryo. J Cell Sci. 2007 Oct 15;120(Pt 20):3578-88. Epub 2007 Sep 25. PubMed PMID: 17895363.
24. Gordon-Weeks R, Tong Y, Davies TG, Leggewie G. Restricted spatial expression of a high-affinity phosphate transporter in potato roots. J Cell Sci. 2003 Aug 1;116(Pt 15):3135-44. Epub 2003 Jun 10. PubMed PMID: 12799416.
25. Bodor DL, Valente LP, Mata JF, Black BE, Jansen LE. Assembly in G1 phase and long-term stability are unique intrinsic features of CENP-A nucleosomes. Mol Biol Cell. 2013 Apr;24(7):923-32. doi: 10.1091/mbc.E13-01-0034. Epub 2013 Jan 30. PubMed PMID: 23363600.
26. De Faveri LE, Hurst CD, Platt FM, Taylor CF, Roulson JA, Sanchez-Carbayo M, et al. Putative tumour suppressor gene necdin is hypermethylated and mutated in human cancer. Br J Cancer. 2013 Apr 2;108(6):1368-77. doi: 10.1038/bjc.2013.104. PubMed PMID: 23549060.
27. Machado E, Swevers L, Sdralia N, Medeiros MN, Mello FG, Iatrou K. Prostaglandin signaling and ovarian follicle development in the silkmoth, Bombyx mori. Insect Biochem Mol Biol. 2007 Aug;37(8):876-85. Epub 2007 Apr 14. PubMed PMID: 17628286.
28. Meseroll RA, Howard L, Gladfelter AS. Septin ring size scaling and dynamics require the coiled-coil region of Shs1p. Mol Biol Cell. 2012 Sep;23(17):3391-406. doi: 10.1091/mbc.E12-03-0207. Epub 2012 Jul 5. PubMed PMID: 22767579.
29. Vafopoulou X. Ecdysteroid receptor (EcR) is associated with microtubules and with mitochondria in the cytoplasm of prothoracic gland cells of Rhodnius prolixus (Hemiptera). Arch Insect Biochem Physiol. 2009 Dec;72(4):249-62. doi: 10.1002/arch.20336. PubMed PMID: 19847923.
30. Levy, G.V. et al. (2015) Depletion of the SR-Related Protein TbRRM1 Leads to Cell Cycle Arrest and Apoptosis-Like Death in Trypanosoma brucei. PLoS One. 10 (8): e0136070.

  1. Quick-Filters:
  2. Cited (2)
  3. Highly Validated (7)

Items 1 to 20 of 50 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

alpha Tubulin / TUBA1B (Loading Control) antibody

Figure 1. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-Tubulin antibody (TU-01; green) and anti-Vimentin antibody (VI-01; SM3128P; red). Nucleus is stained with DAPI (blue). Mouse IgG1 TU-01 Purified All Species C, E, ICC/IF, IP, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

Immunohistochemistry: SM568P Alpha Tubulin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Testis followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rat IgG2a YOL1/34 Purified Av, Hu, Mam, Ms, Rt, Ye C, E, ICC/IF, IP, P, R, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. TA308986 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha Tubulin / TUBA1B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TUBA1B mouse monoclonal antibody. (TA506582) Mouse IgG2a OTI3G3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

alpha Tubulin / TUBA1B antibody

Immunoperoxidase of monoclonal antibody to K-ALPHA-1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml] Mouse IgG2b 4D1 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

alpha Tubulin / TUBA1B antibody

Panel A : Immunofluorescence staining of NIH/3T3 ( mouse embryonal fibroblast cell line ) using alpha Tubulin monoclonal antibody, clone TU-01 ( Cat # MAB3826 ; green ) and VIM monoclonal antibody, clone VI-01 ( Cat # MAB3880 ; red ). Nucleus is stained with DAPI ( blue ).
Panel B : Immunofluorescence staining of NIH/3T3 ( mouse embryonal fibroblast cell line ) using alpha Tubulin monoclonal antibody, clone TU-01 ( Cat # MAB3826 ; green ). Nucleus is stained with DAPI ( blue ). Mouse IgG1 TU-01 Por ICC/IF, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

alpha Tubulin / TUBA1B antibody

Immunofluorescent: Tubulin Antibody (TU-01) [NB500-333] - Fig. 2. Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue). Mouse IgG1 TU-01 Aff - Purified Hu, Mam, Ms, Rt ICC/IF, P, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

alpha Tubulin / TUBA1B (426-450) antibody

Human lung stained with Anti-Tubulin alpha antibody. Rabbit Purified Bov, Chk, Frog, Ge, GP, Hu, Ms, Por, Rt IP, P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (426-450) antibody

Human lung stained with Anti-Tubulin alpha antibody. Rabbit Purified Bov, Chk, Frog, Ge, GP, Hu, Ms, Por, Rt IP, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (426-450) antibody

Human lung stained with Anti-Tubulin alpha antibody. Rabbit Purified Bov, Chk, Frog, Ge, GP, Hu, Ms, Por, Rt IP, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (427-441) (Loading Control) antibody

Western Blot of Rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain. Lane 2: rat brain. Lane 3: A431 cells. Lane 4: Jurkat cells. Lane 5: HeLa cells. Load: 35 µg per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none. Rabbit IgG Aff - Purified Av, Hu, Mam E, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

alpha Tubulin / TUBA1B antibody

Predicted band size: 50 kDa . Positive control: Hela and HT29 cell lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: Hela and HT29 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha Tubulin / TUBA1B antibody

Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-α-tubulin (Clone 10D8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgM 10D8 Aff - Purified All Species, Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

alpha Tubulin / TUBA1B antibody

Western blot of A431 cells, using anti-alpha-Tubulin antibody at 1/1000 dilution. A -Tubulin band showed at the predicted MW (~50 kDa). Rabbit RM113 Purified ChIP, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

alpha Tubulin / TUBA1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA1B (RC205008, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA1B. Mouse IgG2a OTI3H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

alpha Tubulin / TUBA1B (N-term) antibody

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by  mAb TU-02 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgM TU-02 Purified Hu, Ms, Por WB
0.1 mg / €240.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

AM08323PU-N at 10 µg/ml staining alpha Tubulin in Pig heart by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue. Mouse IgM TU-16 Purified All Species E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

alpha Tubulin / TUBA1B Mouse IgM 10D8 Biotin All Species, Hu, Ms, Rt ICC/IF, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

alpha Tubulin / TUBA1B Mouse IgM 10D8 Biotin All Species, Hu, Ms, Rt ICC/IF, WB
25 µg / €260.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-?-tubulin (Clone 10D8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgM 10D8 Aff - Purified All Species, Hu, Ms, Rt ICC/IF, WB
25 µg / €210.00
  Acris Antibodies GmbH

Items 1 to 20 of 50 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn