AMACR / RACE antibody

Principal name

AMACR / RACE antibody

Alternative names for AMACR / RACE antibody

Alpha-methylacyl-CoA racemase, P504S, 2-methylacyl-CoA racemase

SwissProt ID

O09174 (Mouse), P70473 (Rat), Q9UHK6 (Human)

Gene ID

23600 (AMACR), 17117 (Amacr), 25284

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of AMACR / RACE antibody

A mitochondrial and peroxisomal enzyme, Alpha-methyacyl-CoA racemase (AMCAR), an enzyme invloved in beta oxidation of branched chain fatty acods and bile salt intermidiates, and is recently identified as a neomarker for prostate cancer. The AMCAR is over expressed in prostate cancer. Several different isoforms have been reported that are produced either by extensinve alternatve splicing of 5 exons or by use of alternate initiation codons. Atleast 2 different transcripts each derived from the 5 exons have been reported, AMCAR I abd AMACR II. The AMCAQR I is the most abundant form and enclodes for a 382 amino acid protein (42kDa) with a PI of 6.0. The other isoform AMACR II has an alternative fifth exon that exhibit significnat homolgy to fumerate hydratase and encoes a 288 amino acid protein with a molecular weight of 32 kDa, PI 9.6. Several other variants of IA and IIA isoforms are charcterized recently (1). The variant lack exon lack exon 3 are designated as IB and IIB. In prostate tumor tissues that overexpressed AMACR, both the A and B forms are over-expressed. The predominant isoform AMCAR IA also has a peroxisomal targetting signal peptide (PTS1), while other varinats are basic in PI and lack the PTS1.
Carcinomas of the transition zone (TZ) constitute approximately 20% of all prostate cancers. The TZ is the site of origin of grade 1 and grade 2 cancers, the most well-differentiated of the Gleason grade tumors, as well as for benign prostatic hyperplasia (BPH). AMACR has been proposed as a new molecular marker for prostate cancer, because the enzyme is reportedly overexpressed in high-grade dysplasias, also termed prostatic intraepithelial neoplasia, a purported precursor of prostatic carcinoma, and in all grades of prostatic carcinoma of the peripheral zone (3). Small interference RNA (siRNA) against AMACR, but not the control inverted siRNA, reduced the expression of AMACR and significantly impaired proliferation of the androgen-responsive PCa cell line LAPC-4 (2) suggesting that AMACR is essential for optimal growth of PCa cells in vitro and that this enzyme has the potential to be a complementary target with androgen ablation in PCa treatment.

General readings

1. Mubiru JN, Shen-Ong GL, Valente AJ, Troyer DA. Gene. 2004 Feb 18;327(1):89-98.
2. Zha S, Ferdinandusse S, Denis S, Wanders RJ, Ewing CM, Luo J, De Marzo AM, Isaacs WB. Cancer Res. 2003 Nov 1;63:7365-76.
3. Leav I, McNeal JE, Ho SM, Jiang Z. Alpha-methylacyl-CoA racemase (P504S) expression in evolving carcinomas within benign
prostatic hyperplasia and in cancers of the transition zone. Hum Pathol. 2003 Mar;34(3):228-33.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AMACR / RACE antibody

  AMACR mouse monoclonal antibody,clone UMAB68  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AMACR mouse monoclonal antibody. (UM800012; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB68 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody,clone UMAB68  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AMACR mouse monoclonal antibody. (UM800012; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB68 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTIF10 (formerly F10)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AMACR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800852) Mouse IgG1 OTIF10 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AMACR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808448)(1:150) Mouse IgG1 OTI2E3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTI3E10 (formerly 3E10)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AMACR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808476)(1:150) Mouse IgG1 OTI3E10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AMACR / RACE (C-term) antibody

AMACR antibody (C-term) (Cat. #AP50158PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMACR antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AMACR / RACE antibody

Formalin-Fixed, Paraffin-Embedded Human prostate carcinoma (10X) Stained with AMACR / p504S Antibody Cat.-No AP33340PU. Rabbit IgG Purified Hu P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

AMACR / RACE antibody

Formalin-Fixed, Paraffin-Embedded Human prostate carcinoma (10X) Stained with AMACR / p504S Antibody Cat.-No AP33340PU. Rabbit IgG Purified Hu P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

AMACR / RACE antibody

Formalin-Fixed, Paraffin-Embedded Human prostate carcinoma (10X) Stained with AMACR / p504S Antibody Cat.-No AP33340PU. Rabbit IgG Purified Hu P, WB
20 µg / €180.00
  Acris Antibodies GmbH

AMACR / RACE antibody

  Rabbit anti-AMACR Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using AMACR antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AMACR / RACE (C-term) antibody

  Goat Anti-AMACR Polyclonal Antibody  
TA329674 (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AMACR / RACE (312-326) antibody

  Goat Anti-AMACR (aa312-326) Polyclonal Antibody  
TA329675 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AMACR / RACE antibody

  Rabbit Polyclonal Anti-AMACR Antibody  
AMACR antibody validated by WB using 721_B cell lysate at 1.0 ug/ml. AMACR is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

AMACR / RACE antibody

  Mouse Monoclonal AMACR (C-terminus) Antibody  
Western blot detection of AMACR(C-terminus) in Rat Liver lysates using AMACR(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 42KDa.Observed band size: 42KDa. Mouse IgG1 2F3-C11-B12 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTIA11 (formerly A11)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AMACR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800813) Mouse IgG1 OTIA11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTI5G8 (formerly 5G8)  
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AMACR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA800837) Mouse IgG1 OTI5G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTI4G5 (formerly 4G5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMACR (RC213437, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMACR. Mouse IgG1 OTI4G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AMACR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808469)(1:150) Mouse IgG1 OTI4D12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTI3H10 (formerly 3H10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AMACR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808471)(1:150) Mouse IgG1 OTI3H10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AMACR / RACE antibody

  AMACR mouse monoclonal antibody, clone OTI6C7 (formerly 6C7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMACR (RC213437, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMACR.(1:2000) Mouse IgG1 OTI6C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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