AMFR / RNF45 antibody

Principal name

AMFR / RNF45 antibody

Alternative names for AMFR / RNF45 antibody

AMF receptor isoform 2, gp78, RING finger protein 45, Autocrine motility factor receptor isoform 2

SwissProt ID

Q9UKV5 (Human)

Gene ID

267 (AMFR), 826730 (ACS6)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of AMFR / RNF45 antibody

E3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.

General readings

1. Kenichiro Araki, et al. J. Biol. Chem. 2009; 284:32305-32311.
2. Huang, B., et al., Biochem. Biophys. Res.
Commun. 212(3):727-742 (1995).
3. Watanabe, H., et al., J. Biol. Chem. 266(20):13442-13448 (1991).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AMFR / RNF45 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AMFR Rabbit Polyclonal antibody. (TA590740) Rabbit IgG Purified Hu, Mky, Ms, Rt E, ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

AMFR / RNF45 (N-term) antibody

(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR Antibody (N-term) (Cat.#AP12067PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AMFR / RNF45 (Center) antibody

AMFR Antibody (Center) (Cat. #AP50160PU-N) western blot analysis in T47D cell line lysates (35 µg/lane).This demonstrates the AMFR antibody detected the AMFR protein (arrow) Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AMFR / RNF45 (isoform 2) antibody

AMFR / RNF45 Mouse IgG2a 3D9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AMFR / RNF45 (isoform 2) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR polyclonal antibody ( Cat # PAB1684 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

AMFR / RNF45 (isoform 2) antibody

AMFR / RNF45 Rabbit Hu, Ms E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

AMFR / RNF45 (isoform 2) antibody

Human HepG2; WB Suggested Anti-AMFR Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate. AMFR is supported by BioGPS gene expression data to be expressed in HepG2.; AMFR antibody - C-terminal region (ARP42995_T100) in Human HepG2 cells using Western Blot Rabbit Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

AMFR / RNF45 antibody

ELISA: AMFR Antibody [35450002] Rabbit Hu E, P, WB
50 µg / €450.00
  Novus Biologicals Inc.

AMFR / RNF45 antibody

Western Blot: AMFR Antibody [NBP1-49986] - Detection of Human gp78 by Western Blot and Immunoprecipitation.  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-gp78 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  gp78 was also immunoprecipitated by rabbit anti-gp78 antibody BL10542, which recognizes an upstream epitope.  For blotting immunoprecipitated gp78 were used.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AMFR / RNF45 (isoform 2)

AMFR / RNF45 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

AMFR / RNF45 (isoform 2)

AMFR / RNF45 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

11 Item(s)

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