Aminoacylase-1 / ACY1 antibody

Principal name

Aminoacylase-1 / ACY1 antibody

Alternative names for Aminoacylase-1 / ACY1 antibody

ACY-1, N-acyl-L-amino-acid amidohydrolase

SwissProt ID

P37111 (Pig), Q03154 (Human), Q99JW2 (Mouse)

Gene ID

95 (ACY1), 396930, 109652 (Acy1)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Dot blot (Dot), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (R), Flow Cytometry (F), Luminex (LMNX)

Background of Aminoacylase-1 / ACY1 antibody

This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased uriry excretion of N-acetylated amino acids. Altertive splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq].

General readings

Sass J.O., et al. (2006) Am. J. Hum. Genet. 78:401-409.
Van Coster RN., et al. (2005) Biochem Biophys Res Commun. 338(3):1322-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aminoacylase-1 / ACY1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503189) Mouse IgG1 OTI1D4 Purified Hu, Ms F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503190) Mouse IgG1 OTI2D3 Purified Can, Hu, Ms, Rt F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503213) Mouse IgG1 OTI2F1 Purified Can, Hu, Mky F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503226) Mouse IgG1 OTI1A12 Purified Can, Hu, Ms, Rt F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503227) Mouse IgG1 OTI1B12 Purified Can, Hu, Ms, Rt F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503228) Mouse IgG1 OTI16C1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

Human Kidney, Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 4F1-B7 Purified Hu E, ICC/IF, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Aminoacylase-1 / ACY1 antibody

Immunoperoxidase of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3ug/ml] Mouse IgG1 4F1-B7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Aminoacylase-1 / ACY1 antibody

Sample (30 ug of whole cell lysate). A: H1299. 10% SDS PAGE. TA308034 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aminoacylase-1 / ACY1 antibody

Predicted band size: 46kDa. Positive control: Mouse kidney and human normal kidney tissue, K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Mouse kidney tissue lysate Lane2: Human normal kidney tissue lysate Lane3: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aminoacylase-1 / ACY1 antibody

Predicted band size: 46kDa. Positive control: Mouse kidney and human normal kidney tissue, K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Mouse kidney tissue lysate Lane2: Human normal kidney tissue lysate Lane3: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aminoacylase-1 / ACY1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (RC201284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1. Mouse IgG2a OTI2B5 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (RC201284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1. Mouse IgG1 OTI1E5 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (RC201284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1. Mouse IgG1 OTI1H9 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminoacylase-1 / ACY1 antibody

ACY1 ELISA with 1E5 Capture (TA600052) and 2B5 Detection (TA700052) Antibodies. Substrate used: Recombinant Human ACY1 (TP301284) Mouse IgG1 OTI1E5 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aminoacylase-1 / ACY1 antibody

ACY1 ELISA with 2B5 Capture (TA600053) and 1E5 Detection (TA700051) Antibodies. Substrate used: Recombinant Human ACY1 (TP301284) Mouse IgG1 OTI2B5 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aminoacylase-1 / ACY1 antibody

ACY1 ELISA with 2B5 Capture (TA600053) and 1E5 Detection (TA700051) Antibodies. Substrate used: Recombinant Human ACY1 (TP301284) Mouse IgG1 OTI1E5 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Aminoacylase-1 / ACY1 antibody

ACY1 ELISA with 1E5 Capture (TA600052) and 2B5 Detection (TA700052) Antibodies. Substrate used: Recombinant Human ACY1 (TP301284) Mouse IgG1 OTI2B5 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Aminoacylase-1 / ACY1 antibody

ACY1 Luminex with 2B5 Capture (TA600053) and 2F1 Detection (TA700053) Antibodies. Substrate used: Recombinant Human ACY1 (TP304079) Mouse IgG1 OTI2F1 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Aminoacylase-1 / ACY1 antibody

Fig 1.: The cell lysates of Jurkat, HepG2, Hela, A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACY1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Fig. 2: The Recombinant Human ACY1 (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACY1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT1E2 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

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