Aminomethyltransferase antibody

Principal name

Aminomethyltransferase antibody

Alternative names for Aminomethyltransferase antibody

AMT, GCST, GCVT, Glycine cleavage system T protein

SwissProt ID

P25285 (Bovin), P48728 (Human), Q8CFA2 (Mouse), Q9TSZ7 (Canfa)

Gene ID

275 (AMT)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)

Background of Aminomethyltransferase antibody

The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). Glycine encephalopathy (GCE) may be due to a defect in any one of these enzymes.The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-2102 D13811.1 1-2102 2103-2117 BC044792.1 3271-3285

General readings

Narisawa A., et al. (2012) Hum. Mol. Genet. 21 (7), 1496-1503Kure S., et al. (2006) Hum. Mutat. 27 (4), 343-352

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aminomethyltransferase antibody

WB Suggested Anti-AMT Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Lung Rabbit IgG Purified Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Aminomethyltransferase antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMT (RC214343, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMT.(1:2000) Mouse IgG1 OTI4C9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminomethyltransferase antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMT (RC214343, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMT.(1:2000) Mouse IgG1 OTI5D12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminomethyltransferase antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807784)(1:150) Mouse IgG1 OTI6F4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aminomethyltransferase (N-term) antibody

Western blot analysis of AMT Antibody (N-term) (AP17103PU-N) in HepG2 cell line lysates (35 µg/lane). AMT (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Aminomethyltransferase antibody

AMT MaxPab polyclonal antibody. Western Blot analysis of AMT expression in human kidney. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Aminomethyltransferase antibody

AMT MaxPab polyclonal antibody. Western Blot analysis of AMT expression in human kidney. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Aminomethyltransferase antibody

Aminomethyltransferase Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Aminomethyltransferase antibody

Aminomethyltransferase Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Aminomethyltransferase antibody

Human Lung; WB Suggested Anti-AMT Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Lung; AMT antibody - N-terminal region (ARP54313_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Aminomethyltransferase antibody

Western Blot: aminomethyltransferase Antibody [NBP1-54766] - This Anti-AMT antibody was used in Western Blot of Fetal Lung tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Aminomethyltransferase antibody

Immunohistochemistry-Paraffin: Aminomethyltransferase Antibody [NBP1-89783] - Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Aminomethyltransferase

Aminomethyltransferase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Aminomethyltransferase (29-403, His-tag)

Aminomethyltransferase Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Aminomethyltransferase (29-403, His-tag)

Aminomethyltransferase Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Aminomethyltransferase

Aminomethyltransferase Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

AMT (Human) IP-WB Antibody Pair

AMT (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

Aminomethyltransferase Lysate(Denatured)

Aminomethyltransferase Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

AMT overexpression lysate

AMT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

AMT overexpression lysate

AMT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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