AMPK beta-1 chain / AMPKb antibody

Principal name

AMPK beta-1 chain / AMPKb antibody

Alternative names for AMPK beta-1 chain / AMPKb antibody

PRKAB1, 5'-AMP-activated protein kinase subunit beta-1

SwissProt ID

P80386 (Rat), P80387 (Pig), Q5BIS9 (Bovin), Q9R078 (Mouse), Q9Y478 (Human)

Gene ID

5564 (PRKAB1), 19079 (Prkab1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Frozen Sections (C)

Background of AMPK beta-1 chain / AMPKb antibody

AMP-activated protein kise (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterodimeric protein serine/threonine kise that is composed of alpha- (catalytic) and beta/gamma- (regulatory) subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. This catalytic subunit contains a classical serine/threonine protein kise domain close to the N-termil. Free catalytic α subunits are usually ictive due to the presence of an autoinhibitory domain that is located in the center of this subunit. When the α subunit forms a functiol complex with the β and γ subunits, this autoinhibitory function no longer blocks the catalytic function. In response to cellular metabolic stresses, AMPK is activated in an AMP-dependent manner by targeted phosphorylation of Thr172 in the α subunit by the upstream kise like LKB1, calmodulin-dependent protein kise kises and transforming growth factor-β-activated kise-1. Activated AMPK phosphorylates many metabolic enzymes to stimulate catabolic pathways, such as ketogenesis, and inhibit abolic pathways, such as protein synthesis. Recent studies also suggest the important role of AMPK in regulating chemotaxis and cytokine secretion in immune cells.

General readings

Hardie D.G., et al. (2007) Nat. Rev. Mol. Cell Biol. 8:774-785Towler M.C., et al. (2007) Circ. Res. 100:328-341

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AMPK beta-1 chain / AMPKb antibody

Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min. Mouse IgG2a 1A7-E11-E9 Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AMPK beta-1 chain / AMPKb antibody

Predicted cell location: Cytoplasm . Positive control: Human liver and stomach cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human liver and stomach cancer tissue using PRKAB1 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AMPK beta-1 chain / AMPKb antibody

Western blot analysis of extracts from RAW264.7 cells, treated with TNF (20ng/ml, 5mins), using PRKAB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AMPK beta-1 chain / AMPKb antibody

Western blot analysis of AMPK ß1 expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AMPK beta-1 chain / AMPKb antibody

293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified polyclonal anti-AMPK antibody. Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection. Rabbit IgG Poly6498 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

AMPK beta-1 chain / AMPKb (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AMPK beta-1 chain / AMPKb antibody

Immunohistochemistry analyzes of AMPKβ1 antibody (Cat.-No.: AP20343PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AMPK beta-1 chain / AMPKb antibody

Immunohistochemistry (IHC) analyzes of AMPKbeta1 antibody (Cat.-No.: AP20479PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AMPK beta-1 chain / AMPKb pSer182 antibody

Immunohistochemistry (IHC) analyzes of p-AMPK1 antibody (Cat.-No.: AP20874PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AMPK beta-1 chain / AMPKb antibody

Immunohistochemistry (IHC) analyzes of p-HER2 antibody (Cat.-No.: AP20964PU-N) in paraffin-embedded human lung adenocarcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AMPK beta-1 chain / AMPKb antibody

Western Blot analysis of PRKAB1 expression in transfected 293T cell line (H00005564-T01) by PRKAB1 MaxPab polyclonal antibody.

Lane 1: PRKAB1 transfected lysate(30.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

AMPK beta-1 chain / AMPKb antibody

Western Blot analysis of PRKAB1 expression in transfected 293T cell line by PRKAB1 monoclonal antibody (M01), clone 3H12-1A10.

Lane 1: PRKAB1 transfected lysate(31.05 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 3H12-1A10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AMPK beta-1 chain / AMPKb antibody

Immunofluorescence of monoclonal antibody to PRKAB1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG 2C3 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AMPK beta-1 chain / AMPKb antibody

PRKAB1 monoclonal antibody (M07), clone 1D3. Western Blot analysis of PRKAB1 expression in Raw 264.7 ( Cat # L024V1 ). Mouse IgG2b 1D3 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AMPK beta-1 chain / AMPKb antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAB1 polyclonal antibody ( Cat # PAB4575 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma . Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

AMPK beta-1 chain / AMPKb pSer181 antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using AMPK beta1 (Phospho-Ser181) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

AMPK beta-1 chain / AMPKb pSer181 antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using AMPK beta1 (Phospho-Ser181) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

AMPK beta-1 chain / AMPKb antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AMPK beta1 (Ab-181) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

AMPK beta-1 chain / AMPKb antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AMPK beta1 (Ab-181) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

AMPK beta-1 chain / AMPKb antibody

Western blot analysis of extracts from Raw264.7 cells, treated with TNF 20ng/ml 5', using PRKAB1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

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