AMPK beta-2 chain / PRKAB2 antibody

Principal name

AMPK beta-2 chain / PRKAB2 antibody

Alternative names for AMPK beta-2 chain / PRKAB2 antibody

5'-AMP-activated protein kinase subunit beta-2

SwissProt ID

O43741 (Human), Q6PAM0 (Mouse), Q9QZH4 (Rat)

Gene ID

5565 (PRKAB2), 108097 (Prkab2), 64562

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Bear

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of AMPK beta-2 chain / PRKAB2 antibody

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

General readings

Souza RP. et al. (2012) J Psychiatr Res. 46:462-468.Jung KC. et al. (2003) Cytogenet Genome Res. 103:202C.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AMPK beta-2 chain / PRKAB2 antibody

  Rabbit polyclonal Anti-PRKAB2 Antibody  
WB Suggested Anti-PRKAB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Eq, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AMPK beta-2 chain / PRKAB2 antibody

  PRKAB2 mouse monoclonal antibody, clone OTI4H4 (formerly 4H4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAB2 (RC208766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAB2.(1:2000) Mouse IgG1 OTI4H4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AMPK beta-2 chain / PRKAB2 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AMPK beta-2 chain / PRKAB2 antibody

Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Platypus, Por, Prim, Rb, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

AMPK beta-2 chain / PRKAB2 antibody

PRKAB2 polyclonal antibody (A01), Lot # 060626JCS1 Western Blot analysis of PRKAB2 expression in HepG2 ( Cat # L019V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

AMPK beta-2 chain / PRKAB2 antibody

PRKAB2 MaxPab polyclonal antibody. Western Blot analysis of PRKAB2 expression in human liver. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

AMPK beta-2 chain / PRKAB2 antibody

PRKAB2 MaxPab polyclonal antibody. Western Blot analysis of PRKAB2 expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

AMPK beta-2 chain / PRKAB2 antibody

PRKAB2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PRKAB2 expression in mouse kidney. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

AMPK beta-2 chain / PRKAB2 antibody

PRKAB2 monoclonal antibody (M01), clone 2G9 Western Blot analysis of PRKAB2 expression in Hela ( Cat # L013V1 ). Mouse IgG2b 2G9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AMPK beta-2 chain / PRKAB2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAB2 polyclonal antibody ( Cat # PAB4576 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma . Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

AMPK beta-2 chain / PRKAB2 antibody

Mouse Liver; PRKAB2 antibody - middle region (ARP56639_P050) validated by WB using Mouse Liver lysate at 1:1000.; PRKAB2 antibody - middle region (ARP56639_P050) in Mouse Liver cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

AMPK beta-2 chain / PRKAB2 antibody

Western blot analysis of AMPK beta 2 expression in 293T (A); HepG2 (B); mouse heart (C); rat heart (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AMPK beta-2 chain / PRKAB2 antibody

Western blot analysis of AMPK beta 2 expression in 293T (A); HepG2 (B); mouse heart (C); rat heart (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AMPK beta-2 chain / PRKAB2 antibody

Western blot analysis of AMPK beta 2 expression in 293T (A); HepG2 (B); mouse heart (C); rat heart (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

PRKAB2 - human gene knockout kit via CRISPR

PRKAB2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PRKAB2 - human gene knockout kit via CRISPR

PRKAB2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PRKAB2 - human gene knockout kit via CRISPR

PRKAB2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PRKAB2 - human gene knockout kit via CRISPR

PRKAB2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AMPK beta-2 chain / PRKAB2 (full length, N-term HIS tag)

  Recombinant protein of human protein kinase, AMP-activated, beta 2 non-catalytic subunit (PRKAB2), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
AMPK beta-2 chain / PRKAB2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

AMPK beta-2 chain / PRKAB2 (1-272, His-tag)

AMPK beta-2 chain / PRKAB2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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