Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)
Background of AMPK gamma-2 chain / PRKAG2 antibody
AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (a) represents the longest transcript and encodes the longest isoform (a, also referred to as PRKAG2-a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Immunofluorescence of monoclonal antibody to PRKAG2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of PRKAG2 expression in transfected 293T cell line (H00051422-T01) by PRKAG2 MaxPab polyclonal antibody.Lane 1: PRKAG2 transfected lysate(36.08 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PRKAG2 expression in transfected 293T cell line (H00051422-T02) by PRKAG2 MaxPab polyclonal antibody.Lane 1: PRKAG2 transfected lysate(37.50 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of PRKAG2 transfected lysate using anti-PRKAG2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PRKAG2 MaxPab mouse polyclonal antibody (B01) (H00051422-B01).
Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti PRKAG2/AMPKγ2 Polyclonal Antibody, Unconjugated (bs-9446R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 63kD. Observed band size: 63kD.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti PRKAG2/AMPKγ2 Polyclonal Antibody, Unconjugated (bs-9446R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Immunohistochemical analysis of AMPK gamma 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of AMPK gamma 2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).