Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of AMPK gamma-3 chain / AMPKG3 antibody
PRKAG3 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pigcounterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAG3 polyclonal antibody ( Cat # PAB4581 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
Western blot analysis of PRKAG3 polyclonal antibody ( Cat # PAB4581 ) in mouse brain tissue lysate . PRKAG3 ( arrow ) was detected using purified PRKAG3 polyclonal antibody ( Cat # PAB4581 ) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Western blot analysis of anti-PRKAG3 Pab in mouse brain tissue lysate. PRKAG3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of PRKAG3 expression in transfected 293T cell line (H00053632-T01) by PRKAG3 MaxPab polyclonal antibody.Lane 1: PRKAG3 transfected lysate(54.30 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-AMPK gamma 3/PRKAG3 Polyclonal Antibody, Unconjugated (bs-3968R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.