Androgen receptor antibody

Principal name

Androgen receptor antibody

Alternative names for Androgen receptor antibody

Dihydrotestosterone receptor, DHTR, NR3C4, Nuclear receptor subfamily 3 group C member 4

SwissProt ID

P10275 (Human), P15207 (Rat), P19091 (Mouse), Q9GKL7 (Pig), Q9TT90 (Canfa)

Gene ID

367 (AR), 24208 (Ar)

Available reactivities

Hu (Human), Ms (Mouse), Prim (Primate), Rt (Rat), Can (Canine), Mky (Monkey), Bov (Bovine), Por (Porcine)

Available hosts

Rat, Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA), Enzyme Immunoassay (E), Flow Cytometry (F), Dot blot (Dot)

Background of Androgen receptor antibody

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. The resulting isoform (2), also referred to as AR45, is shorter, and has a distinct and shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

This product has been used in:Morvillo V et al. Atypical androgen receptor in the human melanoma cell line IIB-MEL-J. Pigment Cell Res 8:135-41 (1995). PubMed PMID: 7567789
Da Silva JA et al. Sex differences in inflammation induced cartilage damage in rodents. The influence of sex steroids. J Rheumatol 21:330-7 (1994). PubMed PMID: 8182645
Kimura N et al. Immunocytochemical localization of androgen receptor with polyclonal antibody in paraffin-embedded human tissues. J Histochem Cytochem 41:671-8 (1993). PubMed PMID: 8468448
Nakada SY et al. The androgen receptor status of neuroendocrine cells in human benign and malignant prostatic tissue. Cancer Res 53:1967-70 (1993). PubMed PMID: 8481896
Rajpert-De Meyts E & Skakkebaek NE Immunohistochemical identification of androgen receptors in germ cell neoplasia. J Endocrinol 135:R1-4 (1992). PubMed PMID: 1474324
Cutolo M et al. Evidence for the presence of androgen receptors in the synovial tissue of rheumatoid arthritis patients and healthy controls. Arthritis Rheum 35:1007-15 (1992). PubMed PMID: 1418016
Brolin J et al. Immunohistochemistry and biochemistry in detection of androgen, progesterone, and estrogen receptors in benign and malignant human prostatic tissue. Prostate 20:281-95 (1992). PubMed PMID: 1376911
Roselli CE et al. Androgen receptor and 5 alpha-reductase activity in the ductuli efferentes and epididymis of adult rhesus macaques. Biol Reprod 44:739-45 (1991). PubMed PMID: 2043744
Takeda H et al. Autoregulation of androgen receptor expression in rodent prostate: immunohistochemical and in situ hybridization analysis. Biochem Biophys Res Commun 177:488-96 (1991). PubMed PMID: 2043134
Takeda H & Chang C Immunohistochemical and in-situ hybridization analysis of androgen receptor expression during the development of the mouse prostate gland. J Endocrinol 129:83-9 (1991). PubMed PMID: 2030333
West NB et al. Localization and regulation of estrogen, progestin and androgen receptors in the seminal vesicle of the rhesus monkey. J Steroid Biochem Mol Biol 37:11-21 (1990). PubMed PMID: 2242343
Takeda H et al. Immunohistochemical localization of androgen receptors with mono- and polyclonal antibodies to androgen receptor. J Endocrinol 126:17-25 (1990). PubMed PMID: 2199591
Chang C et al. Prostate androgen receptor: immunohistological localization and mRNA characterization. J Steroid Biochem 34:311-3 (1989). PubMed PMID: 2626023
Chang CS et al. Fusion proteins containing androgen receptor sequences and their use in the production of poly- and monoclonal anti-androgen receptor antibodies. Endocrinology 125:1097-9 (1989). PubMed PMID: 2666105
General / background references:Clancy AN et al. Immunohistochemical labeling of androgen receptors in the brain of rat and monkey. Life Sci 50:409-17 (1992). PubMed PMID: 1734159

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Androgen receptor antibody

Western Blot: Androgen Receptor Antibody (2H8) [TA336773] - Western blot analysis using Androgen Receptor mouse mAb against Androgen Receptor (aa221-321)-hIgGFc transfected HEK293 cell lysate. Mouse IgG1 2H8 Purified Hu C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Androgen receptor antibody

Immunohistochemical analysis of paraffin-embedded human human testis (left) and prostate cancer (right) tissues using Androgen Receptor mouse mAb with DAB staining. Mouse IgG1 2H8 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Androgen receptor antibody

Immunoperoxidase of monoclonal antibody to AR on formalin-fixed paraffin-embedded human renal cell carcinoma tissue. [antibody concentration 3ug/ml] Mouse IgG1 1G3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Androgen receptor (N-term) antibody

Human prostate carcinoma stained with anti-AR antibody AM20267PU Rabbit IgG SP107 Purified Hu, Ms, Rt P, WB
0.5 ml / €520.00
  Acris Antibodies GmbH

Androgen receptor (N-term) antibody

Human prostate carcinoma stained with anti-AR antibody AM20267PU Rabbit IgG SP107 Purified Hu, Ms, Rt P, WB
1 ml / €750.00
  Acris Antibodies GmbH

Androgen receptor (N-term) antibody

Human prostate carcinoma stained with anti-AR antibody AM20267PU Rabbit IgG SP107 Purified Hu, Ms, Rt P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

Androgen receptor antibody

Human prostate ca. stained with Anti-AR antibody Rabbit Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Androgen receptor antibody

Human prostate ca. stained with Anti-AR antibody Rabbit Purified Hu, Ms, Rt ICC/IF, IP, P, WB
1 ml / €520.00
  Acris Antibodies GmbH

Androgen receptor antibody

Human prostate ca. stained with Anti-AR antibody Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

Androgen receptor (N-term) antibody

TA302952(0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

Androgen receptor (N-term) antibody

TA302952(0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Androgen receptor antibody

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma, using Androgen Receptor (Ab-213) Antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Androgen receptor antibody

Western blot analysis of extracts from LOVO cells, using Androgen Receptor (Ab-650) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Androgen receptor antibody

Western blot analysis of Androgen Receptor expression in LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Androgen receptor antibody

Western blot analysis of extracts from K562 cells, treated with EGF (200ng/ml, 5mins), using Androgen Receptor (Ab-363) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Androgen receptor antibody

Western blot analysis of extracts of various cell lines, using AR antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Androgen receptor antibody

Western Blot: Androgen Receptor [p Ser213, p Ser210] Antibody (156C135.2) [TA336428] - LNCaP cells (passage number 38) were serum-starved for 2 days.  After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or & Mouse IgG1 156C135.2 Purified Hu, Prim C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Androgen receptor antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AR mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809105)(1:150) Mouse IgG1 OTI1D3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Androgen receptor antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AR (RC215316, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AR.(1:2000) Mouse IgG1 OTI2H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Androgen receptor antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AR mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809123)(1:150) Mouse IgG1 OTI2E6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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