Angiomotin antibody

Principal name

Angiomotin antibody

Alternative names for Angiomotin antibody

AMOT, KIAA1071

SwissProt ID

Q4VCS5 (Human), Q8VHG2 (Mouse)

Gene ID

154796 (AMOT)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Chk (Chicken), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Angiomotin antibody

AMOT belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.

General readings

Holmgren,L., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (24), 9208-9213

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Angiomotin antibody

WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Angiomotin antibody

WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 293T cell lysate AMOT is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Angiomotin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMOT (RC220347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMOT. Mouse IgG1 OTI1F11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Angiomotin antibody

Anti-AMOT mouse monoclonal antibody (TA507127) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AMOT(RC220347). Mouse IgG1 OTI1A8 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Angiomotin antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AMOT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507142) Mouse IgG1 OTI3F9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Angiomotin antibody

AMOT polyclonal antibody (A01), Lot # 060613JCS1. Western Blot analysis of AMOT expression in NIH/3T3. Mouse Serum Hu, Ms E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Angiomotin antibody

Angiomotin Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Angiomotin antibody

Western Blot analysis of AMOT expression in transfected 293T cell line (H00154796-T01) by AMOT MaxPab polyclonal antibody.

Lane 1: AMOT transfected lysate(74.25 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Angiomotin antibody

Western Blot analysis of AMOT expression in transfected 293T cell line (H00154796-T02) by AMOT MaxPab polyclonal antibody.

Lane 1: AMOT transfected lysate(72.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Angiomotin antibody

Angiomotin Mouse IgG1 2A8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Angiomotin antibody

Human Heart; WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; AMOT antibody - N-terminal region (ARP34660_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Angiomotin antibody

Human 293T; WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate. AMOT is supported by BioGPS gene expression data to be expressed in HEK293T.; AMOT antibody - N-terminal region (ARP34661_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Angiomotin antibody

Western Blot: AMOT Antibody [NBP1-78208]-Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-Angiomotin antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  Angiomotin was also immunoprecipitated by rabbit anti-Angiomotin antibodies NBP1-78209 and NBP1-78210, which recognize downstream epitopes. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Angiomotin antibody

Western Blot: AMOT Antibody [NBP1-78209]-  Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells.   Affinity purified rabbit anti-Angiomotin antibody  used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  Angiomotin was also immunoprecipitated by rabbit anti-Angiomotin antibodies NBP1-78208_x000d_
 and NBP1-78210 which recognize other epitopes. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Angiomotin antibody

Western Blot: AMOT Antibody [NBP1-78210]- Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells.   Affinity purified rabbit anti-Angiomotin antibody  used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  Angiomotin was also immunoprecipitated by rabbit anti-Angiomotin antibodies NBP1-78208 and NBP1-78209, which recognize upstream epitopes. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Angiomotin (transcript variant 2)

Angiomotin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Angiomotin

Angiomotin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Angiomotin

Angiomotin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Angiomotin

Angiomotin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Angiomotin

Angiomotin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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