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Angiomotin antibody

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Principal name

Angiomotin antibody

Alternative names for Angiomotin antibody

AMOT, KIAA1071

SwissProt ID

Q4VCS5 (Human), Q8VHG2 (Mouse)

Gene ID

154796 (AMOT), 27494 (Amot)

Ncbi ID

9606, NP_573572, NP_001106962.1, NM_133265, NP_695231.2, BC057638

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Chk (Chicken), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Angiomotin antibody

AMOT belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.

General readings

Holmgren,L., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (24), 9208-9213

H00154796-A01 - Angiomotin
AMOT polyclonal antibody (A01), Lot # 060613JCS1. Western Blot analysis of AMOT expression in NIH/3T3.
H00154796-A01 - Angiomotin
AMOT polyclonal antibody (A01), Lot # 060613JCS1 Western Blot analysis of AMOT expression in HeLa ( Cat # L013V1 ).
H00154796-B01P - Angiomotin
Western Blot analysis of AMOT expression in transfected 293T cell line (H00154796-T01) by AMOT MaxPab polyclonal antibody.Lane 1: AMOT transfected lysate(74.25 KDa).Lane 2: Non-transfected lysate.
H00154796-D01P - Angiomotin
Western Blot analysis of AMOT expression in transfected 293T cell line (H00154796-T02) by AMOT MaxPab polyclonal antibody.Lane 1: AMOT transfected lysate(72.50 KDa).Lane 2: Non-transfected lysate.
ARP34661_P050 - Angiomotin
Human 293T; WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate. AMOT is supported by BioGPS gene expression data to be expressed in HEK293T.; AMOT antibody - N-terminal region (ARP34661_P050) in Human 293T cells using Western Blot
ARP34660_P050 - Angiomotin
Human Heart; WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; AMOT antibody - N-terminal region (ARP34660_P050) in Human Heart cells using Western Blot
TA507110 - Angiomotin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMOT (RC220347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMOT.
TA507127 - Angiomotin
Anti-AMOT mouse monoclonal antibody (TA507127) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AMOT(RC220347).
TA507127 - Angiomotin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMOT (RC220347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMOT.
TA507142 - Angiomotin
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AMOT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507142)
TA507142 - Angiomotin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMOT (RC220347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMOT.
TP320347 - Angiomotin
TA337277 - Angiomotin
WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart
TA337278 - Angiomotin
WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 293T cell lysate AMOT is supported by BioGPS gene expression data to be expressed in HEK293T

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Angiomotin antibody
  Rabbit Polyclonal Anti-AMOT Antibody  
WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Angiomotin antibody
  Rabbit Polyclonal Anti-AMOT Antibody  
WB Suggested Anti-AMOT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 293T cell lysate AMOT is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Angiomotin antibody
  AMOT mouse monoclonal antibody, clone OTI1F11 (formerly 1F11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMOT (RC220347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMOT. Mouse IgG1 OTI1F11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
 

TrueMAB

Angiomotin antibody
  AMOT mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)  
Anti-AMOT mouse monoclonal antibody (TA507127) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AMOT(RC220347). Mouse IgG1 OTI1A8 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

Angiomotin antibody
  AMOT mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AMOT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507142) Mouse IgG1 OTI3F9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

Amot - mouse gene knockout kit via CRISPR
Amot - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Amot - mouse gene knockout kit via CRISPR
Amot - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Amot - mouse gene knockout kit via CRISPR
Amot - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Amot - mouse gene knockout kit via CRISPR
Amot - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Angiomotin (transcript variant 2)
  Recombinant protein of human angiomotin (AMOT), transcript variant 2  
Angiomotin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

AMOT overexpression lysate
AMOT overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Amot (GFP-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191), 200 uL, >10^7 TU/mL
Lenti ORF particles, Amot (GFP-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191), 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Amot (Myc-DDK-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191), 200 uL, >10^7 TU/mL
Lenti ORF particles, Amot (Myc-DDK-tagged) - Mouse angiomotin (cDNA clone MGC:67773 IMAGE:2631191), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.
 

13 Item(s)

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13 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (4)
  2. Lysate (1)
  3. Mouse Lenti ORF Particle (2)
  4. Primary Antibody (5)
  5. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (3)
  2. Polyclonal (2)
Target
  1. Angiomotin (6)
Reactivity
  1. Human (Hu) (5)
  2. Bovine (Bov) (2)
  3. Canine (Can) (2)
  4. Equine (Eq) (2)
  5. Guinea Pig (GP) (2)
  6. Mouse (Ms) (2)
  7. Porcine (Por) (2)
  8. Rabbit (Rb) (2)
  9. Rat (Rt) (2)
  10. Zebrafish (Ze) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (5)
Host
  1. Mouse (3)
  2. Rabbit (2)
Images
  1. 1 image (6)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Species (CRISPR)
  1. Mouse (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. Acris - OriGene (13)
Service
  • OriGene EU
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