Angiopoietin-1 antibody

Principal name

Angiopoietin-1 antibody

Alternative names for Angiopoietin-1 antibody

ANG-1, ANG1, ANGPT1, KIAA0003

SwissProt ID

O08538 (Mouse), O18920 (Bovin), O35460 (Rat), Q15389 (Human), Q60FC1 (Canfa), Q9BDY8 (Pig)

Gene ID

284 (ANGPT1), 11600

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rb (Rabbit), Rt (Rat), Por (Porcine), Mky (Monkey), Bov (Bovine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of Angiopoietin-1 antibody

The angiopoietin (Ang) family of growth factors includes four members, all of which bind to the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-1 and Ang-4, activate the Tie2 receptor, whereas Ang-2 and Ang-3 inhibit Ang-1-induced Tie2 phosphorylation. Angiopoietin-1 (Ang-1) is a secreted growth factor which binds to and activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival and capillary morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilises capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high, but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but its ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 and mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descending limbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation.
Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms' kidney tumours and in vascular remodelling after nephrotoxicity. Altogether, existing data suggest that VEGF-A and Angiopoietins not only have quite different roles during vascular development, but also very complementary and coordinated roles.

General readings

Kopczynska E., et al. (2009) Eur J Gynaecol Oncol. 30(6):646-9.Davis S., et al. (1996) Cell. 87(7):1161-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Angiopoietin-1 (N-term) antibody

Formalin-fixed, paraffin-embedded Mouse Liver stained with Angiopoietin-1 antibody Cat.-No AP07808SU-N after heat-induced antigen retrieval. Rabbit Serum Hu, Ms E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Angiopoietin-1 antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human ANGPT1 with DDK (FLAG) tag probed with TA311195 (1ug/ml) in Lane A and probed with anti-DDK Tag (1:1000, Cat# TA50011-100) in Lane C. Mock-transfected HEK293 probed with TA311195 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Angiopoietin-1 antibody

Rabbit anti-Ang-1 was used at 1:500to detect mouse Ang-1 by WB against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells.  Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells.  Rabbit Serum Hu, Ms P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Angiopoietin-1 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and colon cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ANGPT1 antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Angiopoietin-1 antibody

Predicted cell location: Cytoplasm. Positive control: Human liver and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using ANGPT1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Angiopoietin-1 antibody

Immunofluorescent staining of HeLa cells using anti-ANGPT1 rabbit polyclonal antibody (TA590068). Rabbit IgG Purified Can, Hu, Mky E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Angiopoietin-1 antibody

Human recombinant protein fragment corresponding to amino acids 198-498 of human ANGPT1 (NP_001137) produced in E.coli. Mouse IgG1 OTI7F9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Angiopoietin-1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ANGPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804679) Mouse IgG1 OTI8H2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Angiopoietin-1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANGPT1 (RC219398, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANGPT1.(1:500) Mouse IgG1 OTI8C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Angiopoietin-1 (C-term) antibody

Immunohistochemistry: AP20141PU-N ANG1 antibody staining of Paraffin-Embedded Rat lung tissue (smooth muscle of blood vessel). Rabbit IgG Aff - Purified Can, Hu, Rb, Rt P, WB
0.1 ml / €490.00
  Acris Antibodies GmbH

Angiopoietin-1 (176-224) antibody

Western blot analysis of Ang-1 Antibody Cat.-No AP33394PU-N in extracts from HeLa 1 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Angiopoietin-1 (C-term) antibody

ANGPT1 Antibody (C-term) (Cat. #AP50175PU-N) western blot analysis in NCI-H292 cell line lysates (35µg/lane).This demonstrates the ANGPT1 antibody detected the ANGPT1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Angiopoietin-1 Control Peptide

Angiopoietin-1 Purified
50 µg / €190.00
  Acris Antibodies GmbH

Angiopoietin-1 antibody

Angiopoietin-1 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Angiopoietin-1 (C-term) antibody

Top: ANG-1 staining of paraffin-embedded rat lung tissue (smooth muscle of blood vessel) using. Bottom: Detection of ANG-1 from rat cardiac tissue lysate in Western blot assay. Rabbit IgG Aff - Purified Can, Hu, Rb, Rt P, WB
0.1 ml / €490.00
  Neuromics Antibodies

Angiopoietin-1 antibody

ELISA: Angiopoietin 1 Antibody [30450002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

Angiopoietin-1 antibody

Immunohistochemistry-Paraffin: Angiopoietin 1 Antibody (MM0019-26B10) [NB110-85464] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC _x000d_
with anti human Angiopoietin-1. Mouse IgG2 MM0019-26B10 Purified Hu P, WB
0.1 mg / €450.00
  Novus Biologicals Inc.

Angiopoietin-1 antibody

Immunohistochemistry-Paraffin: Angiopoietin 1 Antibody [NB600-629] - Anti-ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pretreatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit IgG Serum Hu, Ms E, P, WB
0.2 ml / €510.00
  Novus Biologicals Inc.

Angiopoietin-1 antibody

Immunohistochemistry-Paraffin: Angiopoietin 1 Antibody [NBP1-90169] - Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Angiopoietin-1 (transcript variant 1)

Angiopoietin-1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HeLa cells

Regular Price: 20 µg / €399.00

Special Price: 20 µg / €239.00

  OriGene Technologies, Inc.

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