Angiopoietin-2 antibody

Principal name

Angiopoietin-2 antibody

Alternative names for Angiopoietin-2 antibody

ANG-2, ANG2, ANGPT2

SwissProt ID

A0A8J8 (Canfa), O15123 (Human), O35462 (Rat), O35608 (Mouse), O77802 (Bovin)

Gene ID

285 (ANGPT2), 11601 (Angpt2), 89805

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Mky (Monkey), Por (Porcine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E)

Background of Angiopoietin-2 antibody

Human Angiopoitin-2 (Ang-2), a 66 kDa protein consisting of 476 amino acid residues (N21-F496), is fused to a N-terminal myc-tag and produced in insect cells.
The angiopoietin (Ang) family of growth factors includes four members, all of which bind to the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-1 and Ang-4, activate the Tie2 receptor, whereas Ang-2 and Ang-3 inhibit Ang-1-induced Tie2 phosphorylation. Angiopoietin-1 (Ang-1) is a secreted growth factor which binds to and activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival and capillary morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilises capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high, but causing vessel regression when VEGF levels are low. Tie -1 is a Tie -2 homologue but its ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 and mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descending limbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms' kidney tumours and in vascular remodelling after nephrotoxicity. Altogether, existing data suggest that VEGF-A and Angiopoietins not only have quite different roles during vascular development, but also very complementary and coordinated roles.

General readings

Maisonpierre PC, Suri C, Jones PF, et al. Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science 1997; 277:55-60.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Angiopoietin-2 (C-term) antibody

Immunohistochemical analysis of paraffin embedded Human placencta sections using ANG2 antibody Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Angiopoietin-2 antibody

  Rabbit polyclonal Angiopoietin 2 antibody  
Rabbit anti-Ang-2 was used at 1:500 to detect mouse Ang-2 in supernatants of mouse-angiopoietin-expressing endothelial cells.  Lane 1 - mock endothelial cells.  Lane 2 - mouse Ang-2 (clone 2-9) expressing cells.  Lane 3 - mouse Ang-1 (clone 1-15) expressing cells.  Lane 4 - mouse Ang-1 (clone 1-8) expressing cells.  Lane 5 - wt endothelial cells.  Rabbit Serum Hu, Ms E, WB
0.2 ml / €387.00
  OriGene Technologies, Inc.

Angiopoietin-2 (C-term) antibody

  Rabbit Polyclonal ANGPT2 Antibody  
Western blot analysis of ANGPT2 in human liver tissue lysate with ANGPT2 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Angiopoietin-2 antibody

  Anti-ANGPT2 rabbit polyclonal antibody  
Predicted band size: 57 kDa . Positive control: Mouse kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 80 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Angiopoietin-2 antibody

  Anti-ANGPT2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ANGPT2 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Angiopoietin-2 antibody

  Rabbit anti-ANGPT2 Polyclonal Antibody  
Western blot analysis of HepG2 cell lysate using ANGPT2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Angiopoietin-2 antibody

  Rabbit Polyclonal Anti-ANGPT2 Antibody  
Host: Rabbit; Target Name: Angpt2; Sample Tissue: Mouse Liver lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

Angiopoietin-2 antibody

  Rabbit Polyclonal Anti-ANG 2 Antibody  
Western blot analysis of ANG 2 expression in HUVEC cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Angiopoietin-2 antibody

  ANGPT2 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7)  
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ANGPT2 mouse monoclonal antibody. (TA806213; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI3H7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Angiopoietin-2 antibody

  ANGPT2 mouse monoclonal antibody, clone OTI4A3 (formerly 4A3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANGPT2 (RC217216, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANGPT2. Mouse IgG1 OTI4A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Angiopoietin-2 (C-term) antibody

ANGPT2 antibody (C-term) (Cat. #AP50176PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the ANGPT2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Angpt2 - mouse gene knockout kit via CRISPR

Angpt2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Angpt2 - mouse gene knockout kit via CRISPR

Angpt2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Angpt2 - mouse gene knockout kit via CRISPR

Angpt2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Angpt2 - mouse gene knockout kit via CRISPR

Angpt2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Angiopoietin-2 (transcript variant 1)

  Purified recombinant protein of Human angiopoietin 2 (ANGPT2), transcript variant 1.  
Angiopoietin-2 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
CHO cells

Regular Price: 20 µg / €399.00

Special Price: 20 µg / €239.00

  OriGene Technologies, Inc.

Angiopoietin-2

Angiopoietin-2 Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain CHO cells
5 µg / €190.00
  Acris Antibodies GmbH

Angiopoietin-2

Angiopoietin-2 Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain CHO cells
20 µg / €330.00
  Acris Antibodies GmbH

ELISA Kits

Catalog No.    

Human Angiopoietin-2 ELISA Kit

Human Angiopoietin-2 ELISA Kit  
1 kit / €389.00
  OriGene Technologies, Inc.

Mouse Angiopoietin-2 ELISA Kit

Mouse Angiopoietin-2 ELISA Kit  
1 kit / €389.00
  OriGene Technologies, Inc.

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