Anillin / ANLN antibody

Principal name

Anillin / ANLN antibody

Alternative names for Anillin / ANLN antibody

Actin-binding protein anillin

SwissProt ID

Q8K298 (Mouse), Q9NQW6 (Human)

Gene ID

54443 (ANLN)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Frozen Sections (C)

Background of Anillin / ANLN antibody

Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis. Before the cell exits into G1, Anillin associates with E-cadherin and is ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C). APC/C recognizes the D-box domain at the N-terminal region of Anillin. Anillin is commonly overexpressed in tumors and may serve as a potential biomarker.Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.

General readings

Oegema K, Savoian MS, Mitchison TJ, Field CM. Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis. J Cell Biol. 2000 Aug 7;150(3):539-52. PMID: 10931866

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Anillin / ANLN antibody

TA303121 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Anillin / ANLN (C-term) antibody

TA305739 (10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Aff - Purified Bov, Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Anillin / ANLN (C-term) antibody

TA305739 (10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Aff - Purified Bov, Hu, Ms E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

Anillin / ANLN antibody

Immunofluorescence of purified MaxPab antibody to ANLN on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Anillin / ANLN antibody

Anillin / ANLN Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

Anillin / ANLN antibody

ANLN polyclonal antibody ( Cat # PAB7058 ) ( 1 µg/ml ) staining of mouse brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Anillin / ANLN antibody

Immunohistochemistry: Anillin Antibody [NB100-1314] -  ANILLIN / Scraps (C Terminus)  Antibody [NB100-1314]-10µg/ml staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat IgG Aff - Purified Hu E, P
0.1 mg / €400.00
  Novus Biologicals Inc.

Anillin / ANLN antibody

Immunohistochemistry-Paraffin: Anillin Antibody [NB100-68216] - FFPE section of human colon carcinoma.  Affinity purified rabbit anti-Anillin used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Anillin / ANLN antibody

Western Blot: Anillin Antibody [NBP1-31060] - Western blot analysis of Anillin Antibody on: A:293T 7.5% SDS PAGE diluted at 1:500 Rabbit IgG Hu Dot, ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Anillin / ANLN

Anillin / ANLN Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Anillin / ANLN

Anillin / ANLN Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ANLN 293T Cell Transient Overexpression Lysate(Denatured)

ANLN 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

12 Item(s)

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