ANKRD50 antibody

Principal name

ANKRD50 antibody

Alternative names for ANKRD50 antibody

KIAA1223, Ankyrin repeat domain-containing protein 50

SwissProt ID

Q9ULJ7 (Human)

Gene ID

57182 (ANKRD50)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E)

Background of ANKRD50 antibody

Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases, such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKRD50 (ankyrin repeat domain 50) is a 1,429 amino acid phosphoprotein that contains nineteen ANK repeats. Conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly and mosquito, ANKRD50 is encoded by a gene that maps to human chromosome 4q28.1. Chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which encodes an expanded glutamine tract in cases of Huntington's disease, is located on chromosome 4. FGFR-3 is also encoded by a gene that maps to human chromosome 4 and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also linked to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ANKRD50 antibody

Western Blot: ANKRD50 Antibody [NBP1-50031] - Detection of Human and Mouse ANKRD50 by Western Blot (h&m) and Immunoprecipitation (h).  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-ANKRD50 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  ANKRD50 was also immunoprecipitated by rabbit anti-ANKRD50 antibody which recognizes a downstream epitope.  For blotting immunoprecipitated ANKRD50, the ReliaBLOT®  Reagents and Procedures were used.  Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ANKRD50 antibody

Western Blot: ANKRD50 Antibody [NBP1-50032] - Detection of Human and Mouse ANKRD50 by Western Blot (h&m) and Immunoprecipitation (h).  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-ANKRD50 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  ANKRD50 was also immunoprecipitated by rabbit anti-ANKRD50 antibody which recognizes an upstream epitope.  For blotting immunoprecipitated ANKRD50, the ReliaBLOT®  Reagents and Procedures were used.  Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ANKRD50 antibody

Immunohistochemistry-Paraffin: ANKRD50 Antibody [NBP1-93594] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ANKRD50

ANKRD50 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ANKRD50

ANKRD50 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ANKRD50 overexpression lysate

ANKRD50 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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