Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)
Background of ANKRD6 / Diversin antibody
Diversin 1 is a 75 kDa protein that acts in both canonical Wnt and Wnt/JNK signaling. Diversin recruits casein kinase 1 epsilon (CK1ε) to the beta-catenin degradation complex of axin/conductin and GSK3beta, thus leading to N-terminal phosphorylation and subsequent degradation of beta-Catenin.
Detection of endogenous Diversin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Div1-6G8 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2