Annexin A5 / ANXA5 antibody

Principal name

Annexin A5 / ANXA5 antibody

Alternative names for Annexin A5 / ANXA5 antibody

Annexin-5, Annexin V, Lipocortin V, Endonexin II, Calphobindin I, Anchorin CII, ANX5, ENX2, PP4, CBP-I, PAP-I, VAC-alpha, Placental anticoagulant protein I, Placental anticoagulant protein 4, Thromboplastin inhibitor, Vascular anticoagulant-alpha

SwissProt ID

P08758 (Human), P14668 (Rat), P17153 (Chick), P48036 (Mouse), P81287 (Bovin)

Gene ID

308 (ANXA5), 11747 (Anxa5), 25673

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Rb (Rabbit), African clawed frog, Ze (Zebrafish), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), EPair for Elisa (EPair)

Background of Annexin A5 / ANXA5 antibody

Annexin A5 (or annexin V) is a cellular protein of the annexin family. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin, and also to inhibit the activity of phospholipase A1. Annexin A5 forms a shield around negatively-charged phospholipid molecules, which blocks the entry of phospholipids into coagulation (clotting) reactions. Antibodies directed against annexin A5 are the cause of the antiphospholipid syndrom in which the formation of the shield is disrupted by antibodies. Without the shield, there is an increased quantity of phospholipid molecules on cell membranes, speeding up coagulation reactions and causing the blood-clotting characteristic of the antiphospholipid antibody syndrome.
Annexin A5 is used as a probe in the annexin A5 affinity assay to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis aswell as other forms of cell death (1,2). Platelets also expose phosphatidyl-serine on their surface when activated, which serves as binding site for various coagulation factors.
Annexin A5 has been shown to interact with kinase insert domain receptor (3) and integrin beta 5 (4).

General readings

Jeong JJ., et al. (2014) J. Biol. Chem. 289 (4), 2469-2481Liu BC., et al. (2013) Am. J. Physiol. Renal Physiol. 305 (3), F304-F313

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Annexin A5 / ANXA5 antibody

Western blot analysis of Annexin V expression in Jurkat cell lysate Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Annexin A5 / ANXA5 antibody

  Rabbit anti-ANXA5 Polyclonal Antibody  
Western blot analysis of extracts of extracts of HeLa cell lines, using ANXA5 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Annexin A5 / ANXA5 antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1F4-1A5 Purified Hu E, ICC/IF, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Annexin A5 / ANXA5 antibody

  Anti-ANXA5 Rabbit Polyclonal Antibody  
Predicted band size: 36 kDa . Positive control: Mouse eyes and heart, Human cervical cancer tissue, OP9 and RAW264.7 cell lysate. Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Annexin A5 / ANXA5 antibody

  Anti-ANXA5 Rabbit Polyclonal Antibody  
Predicted band size: 36 kDa . Positive control: Human cervical cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Annexin A5 / ANXA5 antibody

  Rabbit Polyclonal Anti-ANXA5 Antibody  
WB Suggested Anti-ANXA5 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Annexin A5 / ANXA5 antibody

  Rabbit Polyclonal Anti-ANXA5 Antibody  
WB Suggested Anti-ANXA5 Antibody Titration: 0.625 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Annexin A5 / ANXA5 antibody

  Rabbit Polyclonal Annexin V Antibody  
Immunofluorescent staining of HeLa cells using anti-ANXA5 rabbit polyclonal antibody (TA590069). Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Annexin A5 / ANXA5 antibody

Annexin A5 / ANXA5 Mouse IgG2a VAA-33 Biotin Hu F
100 Tests / €420.00
  Acris Antibodies GmbH

Annexin A5 / ANXA5 antibody

Annexin A5 / ANXA5 Mouse IgG2a VAA-33 FITC Hu F
100 Tests / €440.00
  Acris Antibodies GmbH

Annexin A5 / ANXA5 antibody

Annexin A5 / ANXA5 Mouse IgG2a VAA-33 Purified Hu E, F
0.1 mg / €400.00
  Acris Antibodies GmbH

Annexin A5 / ANXA5 antibody

Immunofluorescence staining of a 7 days old zebrafish embryo. Mouse IgG1 RUU-WAC2A Purified Hu, Ze C, F, ICC/IF, P
0.1 mg / €340.00
  Acris Antibodies GmbH

Annexin A5 / ANXA5 (C-term) antibody

Western blot analysis of Annexin V antibody (C-term) (AP17111PU-N) in SK-Br-3 cell line lysates (35 µg/lane). Annexin V (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Annexin A5 / ANXA5 (C-term) antibody

Immunohistochemical analysis of paraffin embedded rat tissue sections (cardiac muscle) usin Annexin V antibody Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ANXA5 - human gene knockout kit via CRISPR

ANXA5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ANXA5 - human gene knockout kit via CRISPR

ANXA5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ANXA5 - human gene knockout kit via CRISPR

ANXA5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ANXA5 - human gene knockout kit via CRISPR

ANXA5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Annexin A5 / ANXA5

  Recombinant protein of human annexin A5 (ANXA5)  
Annexin A5 / ANXA5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Annexin A5 / ANXA5

  Recombinant protein of human annexin A5 (ANXA5)  
Annexin A5 / ANXA5 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

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