Annexin A5 / ANXA5 antibody
Principal name
Annexin A5 / ANXA5 antibody
Alternative names for Annexin A5 / ANXA5 antibody
Annexin-5, Annexin V, Lipocortin V, Endonexin II, Calphobindin I, Anchorin CII, ANX5, ENX2, PP4, CBP-I, PAP-I, VAC-alpha, Placental anticoagulant protein I, Placental anticoagulant protein 4, Thromboplastin inhibitor, Vascular anticoagulant-alpha
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), EPair for Elisa (EPair)
Background of Annexin A5 / ANXA5 antibody
Annexin A5 (or annexin V) is a cellular protein of the annexin family. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin, and also to inhibit the activity of phospholipase A1. Annexin A5 forms a shield around negatively-charged phospholipid molecules, which blocks the entry of phospholipids into coagulation (clotting) reactions. Antibodies directed against annexin A5 are the cause of the antiphospholipid syndrom in which the formation of the shield is disrupted by antibodies. Without the shield, there is an increased quantity of phospholipid molecules on cell membranes, speeding up coagulation reactions and causing the blood-clotting characteristic of the antiphospholipid antibody syndrome.
Annexin A5 is used as a probe in the annexin A5 affinity assay to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis aswell as other forms of cell death (1,2). Platelets also expose phosphatidyl-serine on their surface when activated, which serves as binding site for various coagulation factors.
Annexin A5 has been shown to interact with kinase insert domain receptor (3) and integrin beta 5 (4).
General readings
Jeong JJ., et al. (2014) J. Biol. Chem. 289 (4), 2469-2481Liu BC., et al. (2013) Am. J. Physiol. Renal Physiol. 305 (3), F304-F313
Western blot analysis of Annexin V expression in Jurkat cell lysate
Immunoperoxidase of monoclonal antibody to ANXA5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to ANXA5 on HeLa cell. [antibody concentration 10 ug/ml]
ANXA5 monoclonal antibody (M01), clone 1F4-1A5 Western Blot analysis of ANXA5 expression in Hela ( Cat # L013V1 ).
Western Blot analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody (M01), clone 1F4-1A5.Lane 1: ANXA5 transfected lysate(35.9 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ANXA5 is approximately 0.03ng/ml as a capture antibody.
Human Placenta; WB Suggested Anti-ANXA5 Antibody Titration: 0.625ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; ANXA5 antibody - N-terminal region (ARP36687_T100) in Human Placenta cells using Western Blot
Human HepG2; WB Suggested Anti-ANXA5 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; ANXA5 antibody - N-terminal region (ARP36573_T100) in Human HepG2 cells using Western Blot
Immunofluorescence of purified MaxPab antibody to ANXA5 on HeLa cell. [antibody concentration 10 ug/ml]
ANXA5 MaxPab polyclonal antibody. Western Blot analysis of ANXA5 expression in human spleen.
Western Blot analysis of ANXA5 expression in transfected 293T cell line (H00000308-T01) by ANXA5 MaxPab polyclonal antibody.Lane 1: ANXA5 transfected lysate(35.2 KDa).Lane 2: Non-transfected lysate.
ANXA5 MaxPab polyclonal antibody. Western Blot analysis of ANXA5 expression in HepG2.
ANXA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ANXA5 expression in mouse liver.
ANXA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ANXA5 expression in human liver.
Western Blot analysis of ANXA5 expression in transfected 293T cell line (H00000308-T01) by ANXA5 MaxPab polyclonal antibody.Lane 1: ANXA5 transfected lysate(35.90 KDa).Lane 2: Non-transfected lysate.
ANXA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ANXA5 expression in HepG2.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle sections using ANXA5 polyclonal antibody ( Cat # PAB7950 ) .
Western blot analysis of HeLa cell lysate. Using ANXA5 polyclonal antibody ( Cat # PAB7950 ) .
Western blot analysis of ANXA5 expression in Jurkat cell lysate.
Recombinant human Annexin A5, 1-320 aa: 15% SDS-PAGE (3 µg)
Western blot analysis of Annexin V antibody (C-term) (AP17111PU-N) in SK-Br-3 cell line lysates (35 µg/lane). Annexin V (arrow) was detected using the purified Pab.
ANXA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ANXA5 expression in mouse liver.
ANXA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ANXA5 expression in human liver.
ANXA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ANXA5 expression in HepG2.
Immunoprecipitation of ANXA5 transfected lysate using anti-ANXA5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ANXA5 MaxPab mouse polyclonal antibody (B01) (H00000308-B01).
Immunohistochemical analysis of paraffin embedded rat tissue sections (cardiac muscle) usin Annexin V antibody
Western blot analysis of Hela cell lysis using Annexin V antibody
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to ANXA5 on HeLa cell. [antibody concentration 10 µg/ml]
Western Blot analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody, clone 1F4-1A5.
ANXA5 monoclonal antibody, clone 1F4-1A5 Western Blot analysis of ANXA5 expression in Hela.
Detection limit for recombinant GST tagged ANXA5 is approximately 0.03ng/ml as a capture antibody.
Immunoprecipitation of ANXA5 transfected lysate using anti-ANXA5 monoclonal antibody and Protein A Magnetic Bead
Immunofluorescence staining of a 7 days old zebrafish embryo.
Human ANXA5 (Annexin A5) recombinant protein
Human ANXA5 (Annexin A5) recombinant protein
Human ANXA5 (Annexin A5) recombinant protein
Immunofluorescent staining of HeLa cells using anti-ANXA5 rabbit polyclonal antibody (TA590069).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANXA5 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: Annexin V Antibody
Predicted band size: 36 kDa . Positive control: Mouse eyes and heart, Human cervical cancer tissue, OP9 and RAW264.7 cell lysate. Recommended dilution: 1/500-2000
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ANXA5 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 36 kDa . Positive control: Human cervical cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of extracts of HeLa cell lines, using ANXA5 antibody.
Immunohistochemistry of paraffin-embedded mouse lung using ANXA5 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ANXA5 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ANXA5 antibody at dilution of 1:100 (200x lens).
WB Suggested Anti-ANXA5 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate
WB Suggested Anti-ANXA5 Antibody Titration: 0.625 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta
- Quick-Filters:
- Cited (1)
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP00113PU-N | Annexin A5 / ANXA5 antibody |
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Rabbit | Purified | Hu, Ms, Rt | IP, P, WB |
0.1 mg /
€400.00
|
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| Acris Antibodies GmbH | |||||||
Published recently! |
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| TA327155 | Annexin A5 / ANXA5 antibody |
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| Rabbit anti-ANXA5 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| AM31146PU-N | Annexin A5 / ANXA5 antibody |
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 |
Mouse | IgG1 | 1F4-1A5 | Purified | Hu | E, ICC/IF, IP, P, WB |
50 µg /
€420.00
|
| Acris Antibodies GmbH | |||||||
| +5 additional images |
Highly validated antibody! |
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| TA321288 | Annexin A5 / ANXA5 antibody |
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| Anti-ANXA5 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321289 | Annexin A5 / ANXA5 antibody |
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| Anti-ANXA5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA341646 | Annexin A5 / ANXA5 antibody |
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| Rabbit Polyclonal Anti-ANXA5 Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
0.1 mg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA341703 | Annexin A5 / ANXA5 antibody |
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| Rabbit Polyclonal Anti-ANXA5 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh | WB |
0.1 mg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA590069 | Annexin A5 / ANXA5 antibody |
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| Rabbit Polyclonal Annexin V Antibody | |||||||
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Rabbit | IgG | Purified | Can, Hu, Mky, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM05645BT-N | Annexin A5 / ANXA5 antibody |
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 |
Mouse | IgG2a | VAA-33 | Biotin | Hu | F |
100 Tests /
€430.00
|
| Acris Antibodies GmbH | |||||||
| AM05645FC-N | Annexin A5 / ANXA5 antibody |
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|
Mouse | IgG2a | VAA-33 | FITC | Hu | F |
100 Tests /
€450.00
|
| Acris Antibodies GmbH | |||||||
| AM05645PU-N | Annexin A5 / ANXA5 antibody |
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|
Mouse | IgG2a | VAA-33 | Purified | Hu | E, F |
0.1 mg /
€410.00
|
| Acris Antibodies GmbH | |||||||
| AM32999PU-N | Annexin A5 / ANXA5 antibody |
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 |
Mouse | IgG1 | RUU-WAC2A | Purified | Hu, Ze | C, F, ICC/IF, P |
0.1 mg /
€340.00
|
| Acris Antibodies GmbH | |||||||
| AP17111PU-N | Annexin A5 / ANXA5 (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€370.00
|
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| Acris Antibodies GmbH | |||||||
| AP23294PU-N | Annexin A5 / ANXA5 (C-term) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€390.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205619 | ANXA5 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205619BN | ANXA5 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205619LP | ANXA5 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205619RB | ANXA5 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305619 | Annexin A5 / ANXA5 |
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| Recombinant protein of human annexin A5 (ANXA5) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
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| OriGene Technologies, Inc. | |||||
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| TP720182 | Annexin A5 / ANXA5 |
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| Recombinant protein of human annexin A5 (ANXA5) | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€399.00
|
|
| OriGene Technologies, Inc. | |||||
