ANO1 / DOG1 antibody

Principal name

ANO1 / DOG1 antibody

Alternative names for ANO1 / DOG1 antibody

Anoctamin-1, Transmembrane protein 16A, DOG-1, ORAOV2, TAOS2, TMEM16A, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Tumor-amplified and overexpressed sequence 2

SwissProt ID

Q5XXA6 (Human), Q8BHY3 (Mouse)

Gene ID

55107 (ANO1), 101772 (Ano1), 3690 (ITGB3)

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of ANO1 / DOG1 antibody

Calcium dependent chloride channels are required for diverse physiological functions such as normal electrolyte and fluid secretion, olfactory perception, neuronal and smooth muscle excitability. Chloride entry in to cell is an electrogenic transport requiring specialized ion channels that are categorized on their basis of activation mechanism. Based on this at least three types of chloride channels have ben described that re regulated by cAMP, Calcium, cell volume changes and by membrane potentials (1). The calcium activated chloride channels (CaCCs) are important for electrolyte secretion, smooth muscle excitability and olfactory perception. There are several proteins that make up CaCCs including CLC3, bestrophins (Bestrophin 1, Bestrophin 2, bestrophin 3,Bestrophin 4), and members of the chloride channels, calcium activated family. The role of CLC3 is not clear as this protein is secreted in to the extracellular medium. The IL4 stimulated bronchial epithelial cells exhibit a marked increase in Ca activated chloride secretion due to increase density of CaCCs. Along with increased density of CaCCs the epithelial cell also induce a group of putative membrane bound proteins including TMTC3, TSPAN8,, KIAA1126, SIDT1 and TMEM16A (3). In vivo inhibition of TMEM16A by siRNA decreased the chloride secretion in cells suggesting some role of TMEM16A in chloride secretion via CaCCs.
TMEM is 986 amino acid protein that is alternatively spliced generating multiple proteins variants with various combination of alternatively spliced regions. All iso-forms maintained a predictable 8 trans-membrane helices with N and C-termini lying on the cytosolic side. TMEM16A is localized in the plasma membrane in bronchial epithelial cells. Several conserved amino acids in TMEM16A are important for CaCCs activity, substitution of R912 to Alanine diminished the currents of chloride channels. TMEM16A is a member of a larger membrane bound protein family that may also have ion channel function such as TMEM16B, other members such a TMEM16F, TMEM16J, and TMEM16K may represent other types of CaCCs (2).

General readings

Katoh,M. (2004) Int. J. Oncol. 24 (5), 1345-1349

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ANO1 / DOG1 antibody

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805184) Mouse IgG1 OTI1C8 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ANO1 / DOG1 antibody

AM11129PU DOG1 antibody staining of Human gastrointestinal stromal tumor Rabbit SP31 Supernatant Hu P
0.5 ml / €540.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

AM11129PU DOG1 antibody staining of Human gastrointestinal stromal tumor Rabbit SP31 Supernatant Hu P
1 ml / €780.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

AM11129PU DOG1 antibody staining of Human gastrointestinal stromal tumor Rabbit SP31 Supernatant Hu P
0.1 ml / €300.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded gastrointestinal stromal tumor (10X) stained with DOG1 Antibody Cat.-No AM32865SU (Clone DG1/447) at 1/200 using peroxidase conjugate and DAB chromogen. Mouse IgG1 DG1/447 Supernatant Hu F, ICC/IF, IP, P, WB
1.0 ml / €360.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded gastrointestinal stromal tumor (10X) stained with DOG1 Antibody Cat.-No AM32865SU (Clone DG1/447) at 1/200 using peroxidase conjugate and DAB chromogen. Mouse IgG1 DG1/447 Supernatant Hu F, ICC/IF, IP, P, WB
0.5 ml / €290.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded gastrointestinal stromal tumor (10X) stained with DOG1 Antibody Cat.-No AM32865SU (Clone DG1/447) at 1/200 using peroxidase conjugate and DAB chromogen. Mouse IgG1 DG1/447 Supernatant Hu F, ICC/IF, IP, P, WB
0.1 ml / €150.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded GIST (20X) stained with DOG1 Antibody Cat.-No. AM10078PU  (Clone DOG1.1). Mouse IgG1 DOG1.1 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded GIST (20X) stained with DOG1 Antibody Cat.-No. AM10078PU  (Clone DOG1.1). Mouse IgG1 DOG1.1 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded GIST (20X) stained with DOG1 Antibody Cat.-No. AM10078PU  (Clone DOG1.1). Mouse IgG1 DOG1.1 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal stromal tumor (GIST) stained with DOG1 Antibody Cat.-No AM50156PU (Clone SPM580). Mouse IgG1 SPM580 Purified Hu F, ICC/IF, P
0.2 mg / €390.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal stromal tumor (GIST) stained with DOG1 Antibody Cat.-No AM50156PU (Clone SPM580). Mouse IgG1 SPM580 Purified Hu F, ICC/IF, P
0.1 mg / €320.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal stromal tumor (GIST) stained with DOG1 Antibody Cat.-No AM50156PU (Clone SPM580). Mouse IgG1 SPM580 Purified Hu F, ICC/IF, P
20 µg / €160.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Human GIST stained with Anti-DOG1 antibody Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Human GIST stained with Anti-DOG1 antibody Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Human GIST stained with Anti-DOG1 antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Western blot analysis of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

Immunofluorescence analysis of HeLa cells, using TM16A antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

Western blot analysis of rat dorsal root ganglion (DRG) lysate: 1. Anti-Anoctamin-1 (extracellular) antibody, (1:200). 2. Anti-Anoctamin-1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

WB Suggested Anti-TMEM16A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

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