ANO1 / DOG1 antibody

Principal name

ANO1 / DOG1 antibody

Alternative names for ANO1 / DOG1 antibody

Anoctamin-1, Transmembrane protein 16A, DOG-1, ORAOV2, TAOS2, TMEM16A, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Tumor-amplified and overexpressed sequence 2

SwissProt ID

Q5XXA6 (Human), Q8BHY3 (Mouse)

Gene ID

55107 (ANO1), 101772 (Ano1)

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of ANO1 / DOG1 antibody

Calcium dependent chloride channels are required for diverse physiological functions such as normal electrolyte and fluid secretion, olfactory perception, neuronal and smooth muscle excitability. Chloride entry in to cell is an electrogenic transport requiring specialized ion channels that are categorized on their basis of activation mechanism. Based on this at least three types of chloride channels have ben described that re regulated by cAMP, Calcium, cell volume changes and by membrane potentials (1). The calcium activated chloride channels (CaCCs) are important for electrolyte secretion, smooth muscle excitability and olfactory perception. There are several proteins that make up CaCCs including CLC3, bestrophins (Bestrophin 1, Bestrophin 2, bestrophin 3,Bestrophin 4), and members of the chloride channels, calcium activated family. The role of CLC3 is not clear as this protein is secreted in to the extracellular medium. The IL4 stimulated bronchial epithelial cells exhibit a marked increase in Ca activated chloride secretion due to increase density of CaCCs. Along with increased density of CaCCs the epithelial cell also induce a group of putative membrane bound proteins including TMTC3, TSPAN8,, KIAA1126, SIDT1 and TMEM16A (3). In vivo inhibition of TMEM16A by siRNA decreased the chloride secretion in cells suggesting some role of TMEM16A in chloride secretion via CaCCs.
TMEM is 986 amino acid protein that is alternatively spliced generating multiple proteins variants with various combination of alternatively spliced regions. All iso-forms maintained a predictable 8 trans-membrane helices with N and C-termini lying on the cytosolic side. TMEM16A is localized in the plasma membrane in bronchial epithelial cells. Several conserved amino acids in TMEM16A are important for CaCCs activity, substitution of R912 to Alanine diminished the currents of chloride channels. TMEM16A is a member of a larger membrane bound protein family that may also have ion channel function such as TMEM16B, other members such a TMEM16F, TMEM16J, and TMEM16K may represent other types of CaCCs (2).

General readings

Katoh,M. (2004) Int. J. Oncol. 24 (5), 1345-1349

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ANO1 / DOG1 antibody

  ANO1 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)  
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805184) Mouse IgG1 OTI1C8 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded GIST (20X) stained with DOG1 Antibody Cat.-No. AM10078PU  (Clone DOG1.1). Mouse IgG1 DOG1.1 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded GIST (20X) stained with DOG1 Antibody Cat.-No. AM10078PU  (Clone DOG1.1). Mouse IgG1 DOG1.1 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded GIST (20X) stained with DOG1 Antibody Cat.-No. AM10078PU  (Clone DOG1.1). Mouse IgG1 DOG1.1 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €180.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal stromal tumor (GIST) stained with DOG1 Antibody Cat.-No AM50156PU (Clone SPM580). Mouse IgG1 SPM580 Purified Hu F, ICC/IF, P
0.2 mg / €410.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal stromal tumor (GIST) stained with DOG1 Antibody Cat.-No AM50156PU (Clone SPM580). Mouse IgG1 SPM580 Purified Hu F, ICC/IF, P
0.1 mg / €320.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal stromal tumor (GIST) stained with DOG1 Antibody Cat.-No AM50156PU (Clone SPM580). Mouse IgG1 SPM580 Purified Hu F, ICC/IF, P
20 µg / €180.00
  Acris Antibodies GmbH

ANO1 / DOG1 antibody

  Rabbit Polyclonal TMEM16A Antibody  
Western blot analysis of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

  Rabbit polyclonal anti-ANO1 / TM16A antibody  
Immunofluorescence analysis of HeLa cells, using TM16A antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

  Rabbit Polyclonal Anti-Anoctamin-1 (extracellular)  
Western blot analysis of rat dorsal root ganglion (DRG) lysate: 1. Anti-Anoctamin-1 (extracellular) antibody, (1:200). 2. Anti-Anoctamin-1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

  Rabbit Polyclonal Anti-TMEM16A Antibody  
WB Suggested Anti-TMEM16A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

  Rabbit Polyclonal Anti-ANO1 Antibody  
ANO1 / DOG1 Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

  Rabbit Polyclonal DOG1/TMEM16A Antibody  
ELISA: DOG1/TMEM16A  Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

ANO1 / DOG1 antibody

  ANO1 mouse monoclonal antibody, clone OTI1H2 (formerly 1H2)  
Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-ANO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803368) Mouse IgG2a OTI1H2 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ANO1 / DOG1 antibody

  ANO1 mouse monoclonal antibody, clone OTI5H7 (formerly 5H7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANO1 (RC229400, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANO1. Mouse IgG1 OTI5H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ANO1 / DOG1 antibody

  ANO1 mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANO1 (RC229400, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANO1. Mouse IgG1 OTI5H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ANO1 / DOG1 antibody

  ANO1 mouse monoclonal antibody, clone OTI3A3 (formerly 3A3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANO1 (RC229400, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANO1. Mouse IgG2a OTI3A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ANO1 / DOG1 antibody

  ANO1 mouse monoclonal antibody, clone OTI1E6 (formerly 1E6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANO1 (RC229400, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANO1. Mouse IgG1 OTI1E6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ANO1 / DOG1 antibody

  ANO1 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANO1 (RC229400, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANO1. Mouse IgG1 OTI2F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ANO1 / DOG1 antibody

Formalin-Fixed Paraffin-Embedded gastrointestinal stromal tumor staiend with D0G1 Antibody Cat.-No AM09094SU (Clone DOG1.1). Mouse IgG DOG1.1 Supernatant Hu P
0.5 ml / €310.00
  Acris Antibodies GmbH

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