ANP32A antibody

Principal name

ANP32A antibody

Alternative names for ANP32A antibody

C15orf1, LANP, MAPM, PHAP1, Mapmodulin, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Putative HLA-DR-associated protein I

SwissProt ID

O35381 (Mouse), P39687 (Human), P49911 (Rat), P51122 (Bovin), Q8HY67 (Canfa)

Gene ID

8125 (ANP32A), 25379 (Anp32a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of ANP32A antibody

This antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins(tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation.Apoptosis is related to many diseases and development. Caspase 9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf 1, which forms an apoptosome that in turn binds to and activate procaspase 9. Activated caspase 9 cleaves and activates the effector caspases (caspase 3, 6 and 7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase 9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

General readings

Matilla A., et al (2005) Cerebellum 4:7-18.
Bai J., et al. (2001) Oncogene 20:2153-2160.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ANP32A antibody

Lanes: Lane1: 10ug mouse cortex brain lysate; Lane2: 25ug mouse cortex brain lysate; Lane3: 40ug mouse cortex brain lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 2000; Gene Name: ANP32A; Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ANP32A (N-term) antibody

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ANP32A antibody AP22856PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ANP32A (C-term) antibody

IHC analysis with AP22857PU-N of human small intestine Rabbit Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ANP32A antibody

Immunoperoxidase of monoclonal antibody to ANP32A on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 3ug/ml] Mouse IgG1 2G11-4A5 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ANP32A antibody

human kidney; Rabbit Anti-ANP32A Antibody. Catalog Number: ARP40203. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; ANP32A antibody - middle region (ARP40203_P050) in human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

ANP32A (Center) antibody

Western blot analysis of PHAP I expression in human Raji cell lysate with PHAP antibody I at 2 ug/mL (lane A) and 4 ug/mL (lane B), respectively. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ANP32A (N-term) antibody

Western blot analysis of PHAP expression in human Raji cell lysate with PHAP antibody at 1 ug/mL. The wide and strong band below PHAP III is a doublelet composed of PHAP I (upper) and PHAP I2a (lower). Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ANP32A (C-term) antibody

Western blot analysis of PHAP I expression in human Raji cell (A), mouse (B) and rat (C) testis tissue llysates with PHAP I antibody at 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ANP32A (C-term) antibody

Western blot analysis of PHAP expression in human Raji cell llysate with PHAP antibody at 1 ug/ml. The two bands Below: PHAP I might be differently spliced isoforms of PHAP. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ANP32A antibody

Western blot analysis of extracts of various cell lines, using ANP32A antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ANP32A antibody

WB Suggested Anti-ANP32A Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate.ANP32A is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ANP32A antibody

Immunohistochemical analysis of paraffin-embedded human kidney tissue using PP32 antibody Cat.-No AM06525SU-N with DAB staining. Mouse IgG1 8D2 Ascites Hu E, P, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

ANP32A (C-term) antibody

Heart: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ANP32A Control Peptide

ANP32A
50 µg / €160.00
  Acris Antibodies GmbH

ANP32A Control Peptide

ANP32A
50 µg / €160.00
  Acris Antibodies GmbH

ANP32A Control Peptide

ANP32A
50 µg / €160.00
  Acris Antibodies GmbH

ANP32A Control Peptide

ANP32A
50 µg / €160.00
  Acris Antibodies GmbH

ANP32A antibody

ANP32A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ANP32A antibody

ANP32A Mouse IgG1 RJ1 Hu ICC/IF, IP, WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

ANP32A antibody

Immunocytochemistry of ANP32A in Raji cells with ANP32A polyclonal antibody ( Cat # PAB12899 ) at 2 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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