AOF2 antibody

Principal name

AOF2 antibody

Alternative names for AOF2 antibody

Lysine-specific histone demethylase 1, KIAA0601, LSD1, Flavin-containing amine oxidase domain-containing protein 2, BRAF35-HDAC complex protein BHC110

SwissProt ID

O60341 (Human), Q6ZQ88 (Mouse)

Gene ID

23028 (KDM1A), 423028 (FAR1)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Mky (Monkey), Rt (Rat), Macaque, Ze (Zebrafish), Bov (Bovine), Por (Porcine), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ChIP assay (ChIP)

Background of AOF2 antibody

Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor. Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptiol activation by coorditing the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes. In contrast, methylation of histone H3 at Lys9 facilitates transcriptiol repression by recruiting HP1. LSD1 is a component of the CoREST transcriptiol co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neurol-specific gene repression in non-neurol cells. In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptiol activation. Therefore, depending on gene context LSD1 can function as either a transcriptiol co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine.

General readings

1 Shi YJ, et.al Mol Cell. 2005 Sep 16;19(6):857-64.
2 Metzger E, et.al Nature. 2005 Sep 15;437(7057):436-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AOF2 antibody

Immunohistochemistry:  LSD1 Antibody (1B2E5) [NB100-1762] - Human Kidney Carcinoma. Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining. Mouse IgG 1B2E5 Ascites Hu C, E, P, WB
0.1 ml / €320.00
  Novus Biologicals Inc.

AOF2 (N-term) antibody

Western blot analysis of anti-LSD1 Pab (Cat. #TA324483) in mouse brain tissue lysate (35ug/lane). LSD1(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AOF2 (C-term) antibody

Western blot analysis of AOF2 (arrow) using LSD1 Antibody (C-term) (Cat.#TA324484). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

Western blot analysis of extracts of PC12 cell lysate using KDM1A antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

Western Blot: LSD1 Antibody (1B2E5) [TA336270] - Analysis of LSD1 expression in Hela (1) and Jurkat (2) whole cell lysates. Mouse IgG1 1B2E5 Purified Hu, Ms, Prim C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AOF2 (N-term) antibody

Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

Western blot analysis of LSD1/AOF2 using Jurkat cell lysate Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

Predicted band size: 95 kDa . Positive control: SW480 and PC-3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: SW480 cell lysate Lane2: PC-3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

TA329082 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

Western blot detection of KDM1/LSD1 in Hela,HCT116 and COS7 cell lysates using KDM1/LSD1 mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa. Mouse IgG1 1E5-H2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

WB was performed on nuclear extracts from HeLa cells (20 ug) using the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

WB was performed using nuclear extracts from HeLa cells (40 ug) and the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu ChIP, E, ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

WB was performed using respectively 150 ug (lane 1) or 50 ug (lane 2) nuclear extracts from HeLa cells and the antibody against LSD1 diluted 1:1,000. The location of the protein of interest is indicated on the left. Rabbit IgG Purified Hu E, IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

WB was performed with the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on purified recombinant LSD1 protein (50 and 25 ng, lane 1 and 2) and on nuclear extracts (50 ug) from HeLa and HEK293 cells (lane 3 and 4, respectively). Figure 1B: WB was performed as described above using nuclear extracts from HeLa cells (HeLa NE , 20 ug). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

Western blot analysis of LSD1 in A549 cell lysate with LSD1 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AOF2 antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and kidney carcinoma (right), showing nuclear localization using LSD1 mouse mAb with DAB staining. Mouse IgG1 1B2E5 Ascites Hu, Mky, Ms E, P, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

AOF2 (800-850) antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Can, Hu, Mky, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AOF2 (100-150) antibody

Heart: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Can, Hu, Macaque, Mky, Ms, Rt, Ze P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AOF2 (Center) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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