AP-1 / c-Jun antibody

Principal name

AP-1 / c-Jun antibody

Alternative names for AP-1 / c-Jun antibody

Transcription factor AP1, Activator protein 1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39

SwissProt ID

O77627 (Bovin), P05411, P05412 (Human), P05627 (Mouse), P12981, P17325 (Rat), P18289, P18870 (Chick), P54864, P56432 (Pig)

Gene ID

3725 (JUN), 36057 (Jra), 16476 (Jun), 24516

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Dros (Drosophila), Mky (Monkey), Vi (Virus), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Dot blot (Dot), ELISA (detection) (E(detection)), Frozen Sections (C), Electrophoretic Mobility Shift Assay (EMSA), ChIP assay (ChIP), Flow Cytometry (F), Luminex (LMNX)

Background of AP-1 / c-Jun antibody

The Dfos/kayak gene encodes a bZIP protein, DFos, required in a large variety of differentiation and morphogenetic processes throughout Drosophila development. Fos and Jun proteins homo- or heterodimerize to form functional AP-1 transcription factor. Mutation in these genes cause phenotypic mutations in Drosophila. Drosophila mutants lacking either Jun or Fos display indistinguishable dorsal open phenotypes, indicating an essential function of both Jun and Fos for embryonic dorsal closure (1). Phenotypic analysis of these mutants reveals that homodimers of Fos or of Jun cannot replace the function of the heterodimeric complex. This defect is not explained by the lower stability of homodimers as compared to heterodimers, because ’pseudo-homodimers’ which are as stable as native Jun-Fos heterodimers cannot substitute for their function. Thus Jun and Fos play complementary roles that are both required for signal transduction and gene activation during dorsal closure. AP-1 transcription factor Dfos and Forkhead Box O transcription factor Foxo are also required downstream of Jun-N-terminal kinase signaling for the apoptotic response to UV-induced DNA damage in the developing Drosophila retina (2). Activation of JNK pathway is also involved along with puckered (puc) expression in wound healing of D. melanogaster wing imaginal disc wound healing and dorsal closure, the cells of the epidermis activates the AP-1 transcription factor comprised of DJUN and DFOS that, in turn, upregulates the expression of the dpp gene. stage-specific steroid-triggered programmed cell death of larval tissues during Drosophila metamorphosis induce the expression of several key genes of Rel/NF-Kappa B and AP-1 (3). Dfos is induced in a stage-specific manner, immediately before this tissue is destroyed while Djun is expressed for many hours before salivary gland cell death (3).
Spatial and temporal regulation of the JNK signaling cascade may be a general mechanism that controls tissue remodeling during morphogenesis and wound healing. Djun and Dfos mRNA are continuously expressed and their abundance levels are transiently regulated by multiple signaling pathways, the peak response coming at 1-2 hours after perturbation. Djun and Dfos, the products of the Drosophila proto-oncongenes Djun and Dfos, are similar in size and sequence to their mammalian counterparts c-Jun and c-Fos and are related to their mammalian counterparts by their antigenic properties. The Dfos is more highly regulated than Djun which is only modulated. The receptor tyrosine kinase pathways positively regulate Dfos and Djun. The cAMP-mediated pathway positively regulates Dfos but negatively regulates Djun. The protein kinase C-activated pathway does not affect Djun whereas it negatively regulates Dfos (4).

General readings

1. Oh IY  et al. Regulation of the Dynamic Chromatin Architecture of the Epidermal Differentiation Complex Is Mediated by a c-Jun/AP-1-Modulated Enhancer. J Invest DermatolN/A:N/A (2014). IP .PubMed: 24468747
2. Maron BA  et al. Upregulation of steroidogenic acute regulatory protein by hypoxia stimulates aldosterone synthesis in pulmonary artery endothelial cells to promote pulmonary vascular fibrosis. Circulation 130:168-79 (2014). Human .PubMed: 25001622
3. Chandra S  et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci 126:3782-95 (2013).PubMed: 23750012
4. Chen D  et al. Aldosterone stimulates fibronectin synthesis in renal fibroblasts through mineralocorticoid receptor-dependent and independent mechanisms. Gene 531:23-30 (2013).PubMed: 23994292
5. Li P  et al. BATF-JUN is critical for IRF4-mediated transcription in T cells. Nature 490:543-6 (2012).PubMed: 22992523
6. Mullenbrock S  et al. Global Expression Analysis Identified a Preferentially Nerve Growth Factor-induced Transcriptional Program Regulated by Sustained Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase (ERK) and AP-1 Protein Activation during PC12 Cell Differentiation. J Biol Chem 286:45131-45 (2011). ChIP ; Rat .PubMed: 22065583
7. Maass PG  et al. A cis-regulatory site downregulates PTHLH in translocation t(8;12)(q13;p11.2) and leads to Brachydactyly Type E. Hum Mol Genet 19:848-60 (2010). ChIP ; Human .PubMed: 20015959
8. Yang Z  et al. Increased c-Jun and Reduced GATA2 Expression Promotes Aberrant Monocytic Differentiation Induced by Activating PTPN11 Mutants. Mol Cell Biol : (2009).PubMed: 19528235
9. Zong Y  et al. ETS family transcription factors collaborate with alternative signaling pathways to induce carcinoma from adult murine prostate cells. Proc Natl Acad Sci U S A 106:12465-70 (2009). IHC-Fr, IHC-P ; Mouse .PubMed: 19592505
10. O'Shaughnessy RF  et al. Akt-dependent Pp2a activity is required for epidermal barrier formation during late embryonic development. Development 136:3423-31 (2009). WB ; Rat .PubMed: 19762425
11. Lemos MC  et al. Genetic background influences embryonic lethality and the occurrence of neural tube defects in Men1 null mice: relevance to genetic modifiers. J Endocrinol 203:133-42 (2009).PubMed: 19587266

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AP-1 / c-Jun antibody

  JUN mouse monoclonal antibody,clone UMAB49  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-JUN monoclonal antibody. (UM800005; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB49 Purified Can, Hu, Mky, Ms, Rt P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

AP-1 / c-Jun antibody

  JUN mouse monoclonal antibody,clone UMAB67  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-JUN mouse monoclonal antibody. (UM800011; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB67 Purified Can, Hu, Mky, Ms, Rt P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

AP-1 / c-Jun antibody

  JUN mouse monoclonal antibody,clone UMAB49  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-JUN monoclonal antibody. (UM800005; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB49 Purified Can, Hu, Mky, Ms, Rt P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

AP-1 / c-Jun antibody

  JUN mouse monoclonal antibody,clone UMAB67  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-JUN mouse monoclonal antibody. (UM800011; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB67 Purified Can, Hu, Mky, Ms, Rt P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

AP-1 / c-Jun antibody

  JUN (c-Jun ) mouse monoclonal antibody, clone OTI3G2 (formerly 3G2)  
Anti-JUN mouse monoclonal antibody (TA500842) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY JUN(RC209804 ). Mouse IgG1 OTI3G2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AP-1 / c-Jun antibody

  Rabbit Polyclonal c-jun Antibody  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-JUN Rabbit Polyclonal antibody. (TA590119) Rabbit IgG Purified Hu Dot, E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AP-1 / c-Jun antibody

  JUN (c-Jun ) mouse monoclonal antibody, clone OTI2D11 (formerly 2D11)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-JUN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800558) Mouse IgG1 OTI2D11 Purified Can, Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AP-1 / c-Jun antibody

  JUN (c-Jun ) mouse monoclonal antibody, clone OTI3C1 (formerly 3C1)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-JUN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800571) Mouse IgG1 OTI3C1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AP-1 / c-Jun antibody

  JUN (c-Jun ) mouse monoclonal antibody, clone OTI9F5 (formerly 9F5)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-JUN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800592) Mouse IgG2b OTI9F5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AP-1 / c-Jun antibody

  JUN (c-Jun ) mouse monoclonal antibody, clone OTI3E2 (formerly 3E2)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-JUN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800594) Mouse IgG2b OTI3E2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AP-1 / c-Jun antibody

  JUN (c-Jun ) mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-JUN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800639) Mouse IgG1 OTI2A9 Purified Can, Hu, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

AP-1 / c-Jun antibody

Immunohistochemical analysis of paraffin-embedded human intima canncer tissues using c-Jun mouse mAb with DAB staining. Mouse IgG1 5B1 Ascites Hu, Mky, Ms E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

AP-1 / c-Jun antibody

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using c-Jun mouse mAb with DAB staining. Mouse IgG1 4H9 Ascites Hu, Ms E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

AP-1 / c-Jun antibody

  Rabbit polyclonal JUN Antibody (Center T93)  
JUN Antibody (Center T93) (Cat. #TA324660) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the JUN antibody detected the JUN protein (arrow). Rabbit IgG Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AP-1 / c-Jun antibody

  Rabbit polyclonal JUN Antibody (C-term)  
JUN Antibody (C-term) (Cat. #TA324683) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the JUN antibody detected the JUN protein (arrow). Rabbit IgG Purified Bov, Chk, Hu, Ms, Por, Rt F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AP-1 / c-Jun antibody

  Rabbit polyclonal Phospho-cJun(S63) Antibody  
Western blot analysis of anti-Phospho-cJun-pS63 Pab (Cat. #TA324767) in mouse  kidney tissue lysate (35ug/lane). cJun-pS63 (arrow) was detected using the purified Pab. Rabbit IgG Purified Bov, Hu, Ms, Rt Dot, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AP-1 / c-Jun antibody

  Rabbit polyclonal Phospho-cJun(S63) Antibody  
Western blot analysis of anti-Phospho-cJun-pS63 Pab (Cat. #TA324768) in mouse brain tissue lysate (35ug/lane). Mouse Phospho-cJun-pS63(arrow) was detected using the purified Pab. Rabbit IgG Purified Hu Dot, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AP-1 / c-Jun antibody

  Rabbit Polyclonal c-Jun Antibody  
Western blot analysis of c-Jun expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AP-1 / c-Jun antibody

  Rabbit Polyclonal c-Jun Antibody  
Western blot analysis of c-Jun expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AP-1 / c-Jun antibody

  Rabbit Polyclonal c-Jun Antibody  
Western blot analysis on Jurkat cell lysate using c-Jun Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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