AP1 complex subunit beta-1 / AP1B1 antibody

Principal name

AP1 complex subunit beta-1 / AP1B1 antibody

Alternative names for AP1 complex subunit beta-1 / AP1B1 antibody

ADTB1, BAM22, CLAPB2, Beta-adaptin 1, Beta 1-adaptin, Adapter-related protein complex 1 subunit beta-1, Adaptor protein complex AP-1 subunit beta-1, Golgi adaptor HA1/AP1 adaptin beta subunit, Clathrin assembly protein complex 1 beta large chain

SwissProt ID

O35643 (Mouse), P52303 (Rat), Q08DS7 (Bovin), Q10567 (Human)

Gene ID

162 (AP1B1), 1213 (CLTC)

Available reactivities

Bov (Bovine), Hu (Human), Rt (Rat), Hst (Hamster), Ms (Mouse), Prim (Primate), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P)

Background of AP1 complex subunit beta-1 / AP1B1 antibody

Coated vesicle populations contain, besides clathrin, the adaptor complexes AP1 and AP2, also known as HAI (HA1) adaptor and HAII (HA2) adaptor or Assembly Protein 1 (AP1) and Assembly Protein 2 (AP2), respectively. Interest has focused on adaptors because they are known to mediate the interaction between clathrin and components of the membrane. Their location between the clathrin shell and the membrane, as revealed by cryoelectron microscopy, is consistent with such a function. In the cell, clathrin-adaptor interactions proceed in a very controlled and specific manner, leading to coated structures each containing only one type of adaptor. Clathrin-coated membranes at or originating from the plasma membrane contain the AP2 adaptor, while the AP1 adaptor appears to be largely restricted to clathrin-coated membranes of the trans-Golgi network. AP1 adaptor consists of four subunits termed beta 1 (formerly beta prime, 110 kDa), gamma (100 kDa), u1 (47 kDa, and omega 1 (20 kDa). AP2 is made up of two 100-110 kDa polypeptides, termed alpha and beta 2 (formerly beta) subunits, u2 (50 kDa), and s2 (17 kDa) subunit. Alpha and beta 2 subunits (alpha and beta 2 adaptins) behave in standard SDS-PAGE like 100-112 kDa polypeptides. Four alpha isoforms (alpha a1, alpha a2, alpha c1 and alpha c2) which are highly homologous in sequence have been described.

General readings

"Characterization of a new member of the human beta-adaptin gene family from chromosome 22q12, a candidate meningioma gene." Peyrard M., Fransson I., Xie Y.-G., Han F.-Y., Ruttledge M.H., Swahn S., Collins J.E., Dunham I., Collins V.P., Dumanski J.P. Hum. Mol. Genet. 3:1393-1399(1994)

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AP1 complex subunit beta-1 / AP1B1 antibody

Sample(30 ug of whole cell lysate). A:MOLT4. 7.5% SDS PAGE. TA308828 diluted at 1:500 Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit beta-1 / AP1B1 antibody

Predicted cell location: Cytoplasm. Positive control: Human renal and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human renal cancer tissue using AP1B1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 100) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit beta-1 / AP1B1 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue.Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AP1B1 antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit beta-1 / AP1B1 (C-term) antibody

WB Suggested Anti-AP1B1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle Rabbit Aff - Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit beta-1 / AP1B1 antibody

Western blot analysis on human brain lysate under reducing conditions using AP1B1 polyclonal antibody ( Cat # PAB14335 ) at a dilution of 1 : 100. This antibody labels a major protein of approximately 100 kDa of molecular weight and several minor proteins around 70 kDa in both rat and human tissue. The presence of the two lower bands ( IgG fragments ) is due to the magnetic bead purification method employed. Rabbit Purified Hu, Rt C, P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

AP1 complex subunit beta-1 / AP1B1 (C-term) antibody

AP1 complex subunit beta-1 / AP1B1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

AP1 complex subunit beta-1 / AP1B1 antibody

AP1 complex subunit beta-1 / AP1B1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AP1 complex subunit beta-1 / AP1B1 (transcript variant 1)

AP1 complex subunit beta-1 / AP1B1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

AP1 complex subunit beta-1 / AP1B1

AP1 complex subunit beta-1 / AP1B1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

AP1 complex subunit beta-1 / AP1B1

AP1 complex subunit beta-1 / AP1B1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

AP1B1 overexpression lysate

AP1B1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

11 Item(s)

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