AP1 complex subunit gamma-1 / AP1G1 antibody

Principal name

AP1 complex subunit gamma-1 / AP1G1 antibody

Alternative names for AP1 complex subunit gamma-1 / AP1G1 antibody

ADTG, CLAPG1, gamma 1 Adaptin, gamma Adaptin, Adapter-related protein complex 1 subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Golgi adaptor HA1/AP1, Clathrin assembly protein complex 1 gamma-1 large chain

SwissProt ID

B2RYN6 (Rat), O43747 (Human), P22892 (Mouse)

Gene ID

164 (AP1G1), 6316424 (IPR003180), 11765

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Chimp (Chimpanzee), Orang-Utan, Ze (Zebrafish), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Immunoelectron Microscopy (EM), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Whole mount Immunohistochemistry (IHC-wm)

Background of AP1 complex subunit gamma-1 / AP1G1 antibody

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. AP1G1 is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene.

General readings

Peyrard, M.; Parveneh, S.; Lagercrantz, S.; Ekman, M.; Fransson, I.; Sahlen, S.; Dumanski, J. P.: Cloning, expression pattern, and chromosomal assignment to 16q23 of the human gamma-adaptin gene (ADTG). Genomics 50: 275-280, 1998.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AP1 complex subunit gamma-1 / AP1G1 antibody

WB Suggested Anti-AP1G1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit gamma-1 / AP1G1 antibody

Human HepG2; Host: Rabbit . Target Name: AP1G1 . Sample Tissue: HepG2 . Antibody Dilution: 1.0ug/ml. AP1G1 is supported by BioGPS gene expression data to be expressed in HepG2.; AP1G1 antibody - C-terminal region (ARP51923_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

AP1 complex subunit gamma-1 / AP1G1 (646-659) antibody

WB using Anti-AP1G1 antibody shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 µg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 µg of each lysate. Primary antibody was used at 1:500. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG. Goat IgG Aff - Purified Chimp, Hu, Orang-Utan E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit gamma-1 / AP1G1 antibody

Predicted cell location: Cytoplasm . Positive control: Human cervical and ovarian cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human cervical and ovarian cancer tissue using AP1G1 antibody at dilution 1/16, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit gamma-1 / AP1G1 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AP1G1 antibody at dilution 1/55, on the right is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AP1G1 antibody at dilution 1/55. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit gamma-1 / AP1G1 antibody

WB Suggested Anti-Ap1g1 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Lung Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AP1 complex subunit gamma-1 / AP1G1 antibody

AP1 complex subunit gamma-1 / AP1G1 Mouse IgG2b IMD-113 Purified Hu, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

AP1 complex subunit gamma-1 / AP1G1 (646-659) antibody

AP07558PU-N AP1G1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Lung, Respiratory Epithelium at 2.5 µg/ml followed by biotinylated anti-Goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Can, Chk, Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AP1 complex subunit gamma-1 / AP1G1 Control Peptide

AP1 complex subunit gamma-1 / AP1G1
50 µg / €190.00
  Acris Antibodies GmbH

AP1 complex subunit gamma-1 / AP1G1 antibody

Western blot using AP1G1 polyclonal antibody ( Cat # PAB10221 ) shows strong detection of a 91 kDa band corresponding to human AP1G1 in a HeLa whole cell lysate ( lane 1 ).
Peptide competition ( using 1 µg/ml of the immunizing peptide ) blocks the specific reactivity of this antibody with AP1G1 ( lane 2 ).
Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of AP1G1 polyclonal antibody.
Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 60-sec exposure time. Chimp, Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

AP1 complex subunit gamma-1 / AP1G1 antibody

Immunohistochemistry using DAB substrate showing intense staining in the rat airway epithelium using AP1G1 polyclonal antibody ( Cat # PAB14334 ) at a dilution of 1 : 500. Secondary antibody was biotinylated goat and rabbit at a dilution of 1 : 3000 followed by Vector ABC. This antigen has a similar distribution to clathrin, with which it associates. Rabbit Purified Hu, Rt C, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

AP1 complex subunit gamma-1 / AP1G1 antibody

Western Blot: Gamma Adaptin Antibody [NB110-7877] - Western blot using Affinity Purified anti-AP1G1 antibody shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (Lanes 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (Lanes 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time. Goat IgG Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

AP1 complex subunit gamma-1 / AP1G1 antibody

Western Blot: Gamma Adaptin Antibody [NBP1-57633] - This Anti-AP1G1 antibody was used in Western Blot of Fetal Brain tissue lysate at a concentration of 0.5ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AP1 complex subunit gamma-1 / AP1G1 (transcript variant 1)

AP1 complex subunit gamma-1 / AP1G1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

14 Item(s)

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