AP2A1 / ADTAA antibody

Principal name

AP2A1 / ADTAA antibody

Alternative names for AP2A1 / ADTAA antibody

CLAPA1, Alpha adaptin A, Alpha-1 adaptin, AP2 complex subunit alpha-1, Adapter-related protein complex 2 alpha-1 subunit, Adaptor protein complex AP-2 subunit alpha-1, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit, Clathrin assembly protein complex 2 alpha-A large chain

SwissProt ID

O95782 (Human), P17426 (Mouse)

Gene ID

160 (AP2A1)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Prim (Primate), Rt (Rat), Amph (Amphibian), Chk (Chicken), Por (Porcine), Mky (Monkey), Chimp (Chimpanzee), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Neutralisation (NEUT)

Background of AP2A1 / ADTAA antibody

In eukaryotic cells, clathrin-coated vesicles mediate the selective internalization of cell-surface receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP1 and AP2, also known as HAI (HA1) adaptor and HAII (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. Interest has focused on adaptors because they are known to mediate the interaction between clathrin and components of the membrane. Their location between the clathrin shell and the membrane, as revealed by cryoelectron microscopy, is consistent with such a function. Under physiological conditions, in vitro adaptory proteins readily bind to clathrin and thereby induce its polymerization into regular polyhedral coat structures, very similar to the coats of coated vesicles. Clathrin-coated membranes at or originating from the plasma membrane contain the AP2 adaptor, while the AP1 adaptor appears to be largely restricted to clathrin-coated membranes of the trans-Golgi network. Both the Golgi associated AP1 adaptor and the plasma-membrane associated AP2 adaptor are heterotetrameric protein complexes. AP2 adaptor is made up of two 100-110 kD polypeptides, termed alpha and beta 2 (formerly beta) subunits, F2 (50 kD), and omega 2 (17 kD) subunit. alpha and beta 2 subunits (alpha and beta 2 adaptins) behave in standard SDS-PAGE like 100-112 kD polypeptides. Four alpha isoforms (alpha a1, alpha a2, alpha c1 and alpha c2) which are highly homologous in sequence have been described. beta 1 and beta 2 adaptins are highly homologous which suggests that the beta type adaptor chains may mediate the interaction of their adaptors with the highly conserved clathrin molecule. The alpha and gamma adaptins share only 25% overall identity. The other adaptor subunits of the two adaptor complexes which appear to be unrelated, are believed to possess binding sites for different cargo molecules (receptors) and for proteins which might regulate the complex functions of the adaptors. Monoclonal antibodies reacting specifically against adaptor proteins are useful tools for studies on the intracellular distribution and structural relationship of adaptor complexes.

General readings

Takei K et al. Generation of coated intermediates of clathrin-mediated endocytosis on protein-free liposomes. Cell 94:131-41 (1998). PubMed PMID: 9674434
van Delft S et al. Association and colocalization of Eps15 with adaptor protein-2 and clathrin. J Cell Biol 136:811-21 (1997). PubMed PMID: 9049247
Okabayashi Y et al. Interaction of Shc with adaptor protein adaptins. J Biol Chem 271:5265-9 (1996). PubMed PMID: 8617812
De Camilli P & Takei K Molecular mechanisms in synaptic vesicle endocytosis and recycling. Neuron 16:481-6 (1996). PubMed PMID: 8785046
Benmerah A et al. The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP-2. J Cell Biol 131:1831-8 (1995). PubMed PMID: 8557749
Ball CL et al. Expression and localization of alpha-adaptin isoforms. J Cell Sci 108 (Pt 8):2865-75 (1995). PubMed PMID: 7593326
Chang MP et al. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. EMBO J 12:2169-80 (1993). PubMed PMID: 8491205
Sorkin A & Carpenter G Interaction of activated EGF receptors with coated pit adaptins. Science 261:612-5 (1993). PubMed PMID: 8342026
Wong DH & Brodsky FM 100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties. J Cell Biol 117:1171-9 (1992). PubMed PMID: 1607381
Robinson MS 100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies. J Cell Biol 104:887-95 (1987).

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AP2A1 / ADTAA (706-727) antibody

Staining of Human Frontal Cortex lysate using AP2A1 Antibody Cat.-No AP32069PU-N at 0.01 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

AP2A1 / ADTAA antibody

WB Suggested Anti-AP2A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AP2A1 / ADTAA antibody

AP2A1 polyclonal antibody (A01), Lot # 060111JC01 Western Blot analysis of AP2A1 expression in MES-SA/Dx5 ( Cat # L021V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

AP2A1 / ADTAA antibody

Western blot using AP2A1 polyclonal antibody ( Cat # PAB9935 ) shows detection of a band just below 100 kDa correspond-ing to Human AP2A1 in a various preparations.
Lane 1 - HeLa nuclear extract.
Lane 2 - HeLa.
Lane 3 - 293.
Lane 4 - A-431.
Lane 5 - Jurkat whole cell lysates.
In lanes 6-10 the antibody was preincubated with 1 µg/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A1.
Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP2A1 polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 60-sec exposure time. Chimp, Chk, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

AP2A1 / ADTAA (C-term) antibody

AP2A1 / ADTAA Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

AP2A1 / ADTAA antibody

AP2A1 / ADTAA Mouse IgG2a ADA-1 Ascites Hu, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

AP2A1 / ADTAA (all alpha forms) antibody

Figure 1 shows immunolocalization of Alpha 1 Fetoprotein in NRK cells using NB300-721. Mouse IgG2a AC1-M11 Ascites Amph, Bov, Chk, Hu, Mky, Ms, Por, Rt ICC/IF, IP, WB
0.5 ml / €400.00
  Novus Biologicals Inc.

AP2A1 / ADTAA (all alpha forms) antibody

AP2A1 / ADTAA Mouse IgG1 AP6 Purified Bov, Hu, Mky, Ms, Rt ICC/IF, IP, NEUT, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

AP2A1 / ADTAA antibody

AP2A1 / ADTAA Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AP2A1 / ADTAA

AP2A1 / ADTAA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

AP2A1 / ADTAA

AP2A1 / ADTAA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

AP2A1 overexpression lysate

AP2A1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

12 Item(s)

per page
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