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AP2A2 / HIP9 antibody

Principal name

AP2A2 / HIP9 antibody

Alternative names for AP2A2 / HIP9 antibody

AP-2 complex subunit alpha-2, Alpha-2-adaptin, Alpha-adaptin C, ADTAB, CLAPA2, HYPJ, KIAA0899, Huntingtin-interacting protein 9, Adapter-related protein complex 2 alpha-2 subunit, Adaptor protein complex AP-2 subunit alpha-2, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit,lathrin assembly protein complex 2 alpha-C large chain, 100 kDa coated vesicle protein C, Huntingtin-interacting protein J, Huntingtin yeast partner J

SwissProt ID

O94973 (Human)

Gene ID

161 (AP2A2)

Ncbi ID

NP_036437, NP_036437.1, NM_012305

Available reactivities

Hu (Human), Can (Canine), Chimp (Chimpanzee), Chk (Chicken), Ms (Mouse), Orang-Utan, Rt (Rat), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of AP2A2 / HIP9 antibody

AP2A2 is a component of the adaptor protein complex 2 (AP-2). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of syptic vesicle membranes from the presyptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis sigl motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-termil appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif .

Human Lung; WB Suggested Anti-AP2A2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung; AP2A2 antibody - C-terminal region (ARP59974_P050) in Human Lung cells using Western Blot

ARP59974_P050

WB using Anti-AP2A antibody shows detection of a band just below 100 kDa correspond-ing to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 µg/ml of the immunizing peptide which effect-ively blocks the specific reactivity of this antibody with AP2A. Primary antibody was used at 1:500.

TA319408

Western blot analysis of AP2A2 Antibody (Center) (Cat. #TA325045) in HepG2 cell line lysates (35ug/lane). AP2A2 (arrow) was detected using the purified Pab.

TA325045

Formalin-fixed and paraffin-embedded human hepatocarcinoma with AP2A2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TA325045

Flow cytometric analysis of HepG2 cells using AP2A2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TA325045

Western blot analysis of extracts of various cell lines, using AP2A2 antibody.

TA332663

WB Suggested Anti-AP2A2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Lung

TA342803

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA319408	AP2A2 / HIP9 (3-14) antibody
	Goat polyclonal anti-AP2A antibody
	Goat	IgG	Aff - Purified	Can, Chimp, Chk, Hu, Ms, Orang-Utan, Rt	E, WB	0.1 mg / €503.00
						OriGene Technologies, Inc.
TA325045	AP2A2 / HIP9 (Center) antibody
	Rabbit polyclonal AP2A2 Antibody (Center)
	Rabbit			Hu	WB	0.4 ml / €410.00
						OriGene Technologies, Inc.
+2 additional images
TA332663	AP2A2 / HIP9 antibody
	Rabbit anti-AP2A2 Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 mg / €335.00
						OriGene Technologies, Inc.
TA342803	AP2A2 / HIP9 antibody
	Rabbit Polyclonal Anti-AP2A2 Antibody
	Rabbit	IgG	Purified	Can, GP, Hu, Ms, Por, Rt	WB	50 µg / €360.00
						OriGene Technologies, Inc.

CRISPR

Catalog No.	Species	Donor Vector
KN203018	AP2A2 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN203018BN	AP2A2 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN203018LP	AP2A2 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN203018RB	AP2A2 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Lysates

Catalog No.
LY415866	AP2A2 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC203018L2V	Lenti ORF particles, AP2A2 (mGFP-tagged) - Human adaptor-related protein complex 2, alpha 2 subunit (AP2A2), 200 uL, >10^7 TU/mL
		200 µl / €1,082.00
		OriGene Technologies, Inc.

RC203018L1V	Lenti ORF particles, AP2A2 (Myc-DDK tagged) - Human adaptor-related protein complex 2, alpha 2 subunit (AP2A2) , 200 uL, >10^7 TU/mL
		200 µl / €969.00
		OriGene Technologies, Inc.

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