APAF1 antibody

Principal name

APAF1 antibody

Alternative names for APAF1 antibody

Apaf-1, KIAA0413, Apoptotic protease-activating factor 1

SwissProt ID

O14727 (Human), O88879 (Mouse), Q9EPV5 (Rat), Q9GL23 (Canfa)

Gene ID

317 (APAF1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Chicken, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of APAF1 antibody

Large multi-protein complexes known as the DISC (death inducing signaling complex) and the apoptosome activate the normally inactive caspases through proteolytic processing (1). The formation of these complexes leads to the activation of caspases and the execution of apoptosis (1,2). The apoptosome is a large 700 or 1400 kDa protein complex comprised of Apaf 1 (apoptotic protease activating factor-1), cytochrome c, and dATP (1). Apaf 1 and cytochrome c in the presence of dATP leads to the oligomerization of Apaf 1 and the formation of either the 700 or 1400 kDa complexes (3&4). Once formed the apoptosome activates caspase-9, which in turn activates the effector caspases (1&5). Some researchers have found the 700 kDa apoptosome to be the most effective activator of the effector caspases, when compared to the 1400 kDa apoptosome (3&4). Apaf 1 has also been shown to be a target of regulation by a variety of proteins that include p53, IAPs, heat shock proteins and Smac/Diablo (1&6).

General readings

General / background references:Cecconi F et al. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94:727-37 (1998). PubMed PMID: 9753320

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APAF1 (C-term) antibody

Human Spleen (formalin-fixed, paraffin-embedded) stained with APAF1 antibody AP22708PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

APAF1 antibody

Human testis stained with anti-Apaf-1 antibody Rabbit Purified Hu, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

APAF1 antibody

Human testis stained with anti-Apaf-1 antibody Rabbit Purified Hu, Ms, Rt P
1 ml / €440.00
  Acris Antibodies GmbH

APAF1 antibody

Human testis stained with anti-Apaf-1 antibody Rabbit Purified Hu, Ms, Rt P
0.1 ml / €180.00
  Acris Antibodies GmbH

APAF1 (N-term) antibody

Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APAF1 (C-term) antibody

Western blot analysis of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1 ug/ml dilution in the absence (A) or presence (B) of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APAF1 antibody

Western blot analysis of extracts from COLO205, using APAF-1-ALT antibody (#TA313453).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APAF1 antibody

Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/mL. Mouse 2E10 Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APAF1 antibody

Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 ug/mL. Mouse IgG 5E11 Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

APAF1 antibody

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using APAF1 Antibody at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APAF1 antibody

Gel: 6%SDS-PAGE,Lysate: 40 µg,Lane 1-3: Hela cells, A549 cells, HUVEC cells,Primary antibody: APAF1 Antibody at dilution 1/350 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 20 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APAF1 antibody

Predicted cell location: Cytoplasm . Positive control: Human brain and cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human brain and cervical cancer tissue using APAF1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

APAF1 antibody

Predicted cell location: Cytoplasm . Positive control: Human cervical and ovarian cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human cervical and ovarian cancer tissue using APAF1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

APAF1 antibody

Western blot analysis on COLO205 cell lysate using APAF1 Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APAF1 (1-421) antibody

APAF1 Mouse IgG1 5C1 Azide Free Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

APAF1 antibody

Western blot analysis using anti-Apaf1 antibody at 1 µg/ml on human heart tissue lysate in the absense (A) and presense (B) of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

APAF1 antibody

APAF1 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

APAF1 antibody

Immunohistochemical staining of human heart with Rabbit anti APAF1 antibody Cat.-No AP05812PU-N Rabbit IgG Purified Hu, Ms P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

APAF1 antibody

Immunohistochemical staining of human heart with Rabbit anti APAF1 antibody Cat.-No AP05812PU-N Rabbit IgG Purified Hu, Ms P, WB
50 µg / €280.00
  Acris Antibodies GmbH

APAF1 antibody

Immunohistochemical analysis ussing APAF1 antibody (Cat.-No. AP06014PU-N) in Paraffin-embedded human lung carcinoma tissue.
	. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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