APBA1 / MINT1 antibody

Principal name

APBA1 / MINT1 antibody

Alternative names for APBA1 / MINT1 antibody

Neuron-specific X11 protein, Amyloid beta A4 precursor protein-binding family A member 1, Neuronal Munc18-1-interacting protein 1, Adapter protein X11alpha

SwissProt ID

B2RUJ5, O35430 (Rat), Q02410 (Human)

Gene ID

83589 (Apba1), 320 (APBA1), 319924

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of APBA1 / MINT1 antibody

APBA1 is part of two multimeric complexes, the first containing Munc18-1 and syntaxin 1 and the second being a brain specific heterotrimeric complex with LIN-10/X11-alpha, LIN-2/CASK, and LIN7. It binds to the cytoplasmic domain of amyloid protein (APP). ABPA1 has a putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. It may modulate processing of the beta amyloid precursor protein (APP) and hence formation of beta APP.
The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three Mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer's Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.

General readings

Borg JP, Ooi J, Levy E, et al. The phosphotyrosine interaction domains of X11 and FE65 bind to distinct sites on the YENPTY motif of amyloid precursor protein. Mol. Cell. Biol. 1996; 16:6229-41.
Mueller HT, Borg JP, Margolis B, et al. Modulation of amyloid precursor protein metabolism by X11alpha/Mint1. A deletion analysis of protein-protein interaction domains. J. Biol. Chem. 2000; 275:39302-6.
Maximov A, Sudhof TC, and Bezprozvanny I. Association of neuronal calcium channels with modular adaptor proteins. J. Biol. Chem. 1999; 274:24453-6
Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APBA1 / MINT1 antibody

TA303223 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APBA1 / MINT1 (N-term) antibody

Western blot analysis of APBA1 in rat brain tissue lysate with APBA1 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APBA1 / MINT1 antibody

WB Suggested Anti-APBA1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Raji cell lysateAPBA1 is supported by BioGPS gene expression data to be expressed in Raji Rabbit IgG Purified Hu, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

APBA1 / MINT1 (N-term) Control Peptide

Western blot analysis of APBA1 in rat brain tissue lysate with APBA1 antibody Cat.-No. AP55509PU-N at 0.5 µg/ml in (A) the absence and (B) the presence of blocking peptide Cat.-No. AP55509CP-N.
50 µg / €160.00
  Acris Antibodies GmbH

APBA1 / MINT1 antibody

APBA1 / MINT1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

APBA1 / MINT1 antibody

APBA1 polyclonal antibody ( Cat # PAB7261 ) ( 0.1 µg/ml ) staining of human brain ( cerebellum ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

APBA1 / MINT1 antibody

Human Intestine; APBA1 antibody - N-terminal region (AVARP13059_P050) in Human Intestine cells using Immunohistochemistry Rabbit Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

APBA1 / MINT1 antibody

NBP1-06015 (0.1ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

APBA1 / MINT1 antibody

APBA1 / MINT1 Rabbit Purified Hu, Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

APBA1 / MINT1 antibody

Immunohistochemistry-Paraffin: APBA1 Antibody [NBP1-84306] - Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

10 Item(s)

per page
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