APC1 / ANAPC1 antibody

Principal name

APC1 / ANAPC1 antibody

Alternative names for APC1 / ANAPC1 antibody

Anaphase-promoting complex subunit 1, TSG24, MCPR, Cyclosome subunit 1, Testis-specific gene 24 protein, Mitotic checkpoint regulator

SwissProt ID

P53995 (Mouse), Q9H1A4 (Human)

Gene ID

64682 (ANAPC1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chimp (Chimpanzee), Chk (Chicken)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of APC1 / ANAPC1 antibody

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.

General readings

General / background references:Kraft C et al. Mitotic regulation of the human anaphase-promoting complex by phosphorylation. EMBO J 22:6598-609 (2003). PubMed PMID: 14657031

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APC1 / ANAPC1 antibody

Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APC1 / ANAPC1 antibody

Immunofluorescence analysis of HepG2 cells, using Cyclosome 1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

APC1 / ANAPC1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using APC1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

APC1 / ANAPC1 pSer377 antibody

AP07834PU-N APC1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Pancreatic Carcinoma at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APC1 / ANAPC1 pSer355 antibody

Western blot using Affinity Purified anti-APC1 pS355 antibody shows detection of a band ~215 kDa corresponding to  phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 µg of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results. Rabbit IgG Aff - Purified Bov, Can, Chimp, Chk, Hu, Ms, Rt E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

APC1 / ANAPC1 pSer355 antibody

APC1 / ANAPC1 Rabbit IgG Aff - Purified Hu E
0.1 mg / €440.00
  Acris Antibodies GmbH

APC1 / ANAPC1 pSer377 antibody

Immunohistochemistry. Affinity purified anti-APC1 pS377 antibody was used at 5.0 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multicancer slides. This image shows moderate positive cytoplasmic and occasional nuclear staining of pancreatic carcinoma cells at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

APC1 / ANAPC1 antibody

Immunohistochemistry Analysis: AP20205PU-N APC1 antibody staining of Paraffin-Embedded Human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P
0.1 mg / €350.00
  Acris Antibodies GmbH

APC1 / ANAPC1 Control Peptide

APC1 / ANAPC1
50 µg / €160.00
  Acris Antibodies GmbH

APC1 / ANAPC1 antibody

Immunohistochemistry of ANAPC1 (phospho S377) polyclonal antibody ( Cat # PAB9936 ) was used at 5.0 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate positive cytoplasmic and occasional nuclear staining of pancreatic carcinoma cells at 60X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA. Rabbit Bov, Hu, Ms, Rt E, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

APC1 / ANAPC1 antibody

Western blot using ANAPC1 (phospho S355) polyclonal antibody ( Cat # PAB10017 ) shows detection of a band ~215 kDa corresponding to phosphorylated human ANAPC1 ( arrowhead ).
Lane 1 shows lysate from asynchronous cells.
Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary.
Lane 3 shows lysate from cells treated with nocodazole to synchronizecells at the M phase.
Phosphorylated ANAPC1 is mostly present only in cell preparations arrested at cell division.
Each lane contains approximately 30 µg of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose.
After blocking with 5% non-fat drymilk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
ECL reagent was used for detection.
Data contributed by Bing Li, UT South western. Rabbit Bov, Chimp, Chk, Hu, Ms, Rt E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

APC1 / ANAPC1 antibody

APC1 / ANAPC1 Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

APC1 / ANAPC1 pSer688 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using APC1 (Phospho-Ser688) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

APC1 / ANAPC1 pSer688 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using APC1 (Phospho-Ser688) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

APC1 / ANAPC1 pSer355 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using APC1 (Phospho-Ser355) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

APC1 / ANAPC1 pSer355 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using APC1 (Phospho-Ser355) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

APC1 / ANAPC1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using APC1 (Ab-688) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

APC1 / ANAPC1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using APC1 (Ab-688) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

APC1 / ANAPC1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using APC1 (Ab-355) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

APC1 / ANAPC1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using APC1 (Ab-355) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

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