APC10 / ANAPC10 antibody

Principal name

APC10 / ANAPC10 antibody

Alternative names for APC10 / ANAPC10 antibody

Anaphase-promoting complex subunit 10, Cyclosome subunit 10

SwissProt ID

Q2YDH1 (Bovin), Q8K2H6 (Mouse), Q9UM13 (Human)

Gene ID

10393 (ANAPC10), 1039306 (aas)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of APC10 / ANAPC10 antibody

Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in telophase and cytokinesis.

General readings

Grossberger R. et al. (1999) Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. J Biol Chem;274:14500-14507.
Huang JN, Park I, Ellingson E, Littlepage LE, Pellman D; (2001) Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p. J Cell Biol;154:85-94.
Passmore, L.A. et al. (2003) Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J.;22:786-796.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APC10 / ANAPC10 (N-term) antibody

Rabbit anti-APC10 was used at 1:500to detect human APC10 in various cell extracts. All lanes contain 20 µg of lysate or extract as follows:  lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat  whole cell lysate; lane 5, 293 whole cell lysate.  Primary antibody was reacted with the membrane at RT for 1 h.  After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 (Center) antibody

Western blot analysis of APC10 in mouse heart tissue lysate with APC10 antibody at (A) 1 and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ANAPC10 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and gastric cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ANAPC10 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 antibody

WB Suggested Anti-ANAPC10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Spleen Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APC10 / ANAPC10 control peptide

APC10 / ANAPC10
50 µg / €160.00
  Acris Antibodies GmbH

APC10 / ANAPC10 antibody

Western Blot analysis of ANAPC10 expression in transfected 293T cell line (H00010393-T01) by ANAPC10 MaxPab polyclonal antibody.

Lane 1: ANAPC10 transfected lysate(20.46 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

APC10 / ANAPC10 antibody

Immunofluorescence of purified MaxPab antibody to ANAPC10 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

APC10 / ANAPC10 antibody

Immunofluorescence of purified MaxPab antibody to ANAPC10 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

APC10 / ANAPC10 antibody

Immunoprecipitation of ANAPC10 transfected lysate using anti-ANAPC10 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ANAPC10 purified MaxPab mouse polyclonal antibody (B02P) (H00010393-B02P). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

APC10 / ANAPC10 antibody

Western Blot analysis of ANAPC10 expression in transfected 293T cell line (H00010393-T02) by ANAPC10 MaxPab polyclonal antibody.

Lane 1: ANAPC10 transfected lysate(21.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

APC10 / ANAPC10 antibody

Immunoblotting of ANAPC10 polyclonal antibody ( Cat # PAB10019 ) was used at a 1:500 dilution to detect human ANAPC10 in various cell extracts.
This antibody clearly detects a ~26 kDa band corresponding to human ANAPC10 ( predicted molecular weight is 21 kDa ).
All lanes contain 20 µg of lysate or extract as follows : lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A-431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate.
Primary antibody was reacted with the membrane at room temperature for 1 h.
After subsequent washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization.
Exposure time was 4 min. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

APC10 / ANAPC10 antibody

Human Spleen; WB Suggested Anti-ANAPC10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; ANAPC10 antibody - N-terminal region (ARP43169_P050) in Human Spleen cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

APC10 / ANAPC10 antibody

Western Blot: Apc10 Antibody [NB600-887] - Immunoblotting. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a- Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa. Rabbit IgG Aff - Purified Hu, Ms E, IP, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

APC10 / ANAPC10 antibody

APC10 / ANAPC10 Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

APC10 / ANAPC10 antibody

Immunohistochemistry-Paraffin: Apc10 Antibody [NBP1-77157] - Mouse heart tissue with APC10 antibody at 5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APC10 / ANAPC10

APC10 / ANAPC10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

APC10 / ANAPC10

APC10 / ANAPC10 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

APC10 / ANAPC10

APC10 / ANAPC10 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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