APC10 / ANAPC10 antibody

Principal name

APC10 / ANAPC10 antibody

Alternative names for APC10 / ANAPC10 antibody

Anaphase-promoting complex subunit 10, Cyclosome subunit 10

SwissProt ID

Q2YDH1 (Bovin), Q8K2H6 (Mouse), Q9UM13 (Human)

Gene ID

10393 (ANAPC10), 68999 (Anapc10)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of APC10 / ANAPC10 antibody

APC10 is a core subunit of the aphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquititing the aphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquititing cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kise-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).[supplied by OMIM, Feb 2011]. Transcript Variant: This variant (1) represents the predomint transcript. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordites used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additiol publications. ##Evidence-Data-START## Transcript exon combition :: BM921310.1, BI668952.1 [ECO:0000332] Rseq introns :: single sample supports all introns ERS025087 [ECO:0000348] ##Evidence-Data-END##

General readings

Grossberger R. et al. (1999) Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. J Biol Chem;274:14500-14507.
Huang JN, Park I, Ellingson E, Littlepage LE, Pellman D; (2001) Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p. J Cell Biol;154:85-94.
Passmore, L.A. et al. (2003) Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J.;22:786-796.

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APC10 / ANAPC10 (N-term) antibody

  Rabbit polyclonal anti-APC10 antibody  
Rabbit anti-APC10 was used at 1:500to detect human APC10 in various cell extracts. All lanes contain 20 µg of lysate or extract as follows:  lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat  whole cell lysate; lane 5, 293 whole cell lysate.  Primary antibody was reacted with the membrane at RT for 1 h.  After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Rabbit Aff - Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 (Center) antibody

  Rabbit Polyclonal APC10 Antibody  
Western blot analysis of APC10 in mouse heart tissue lysate with APC10 antibody at (A) 1 and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 antibody

  Anti-ANAPC10 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ANAPC10 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 antibody

  Anti-ANAPC10 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and gastric cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ANAPC10 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 antibody

  Rabbit Polyclonal Anti-ANAPC10 Antibody  
WB Suggested Anti-ANAPC10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Spleen Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

APC10 / ANAPC10 antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Anapc10 - mouse gene knockout kit via CRISPR

Anapc10 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Anapc10 - mouse gene knockout kit via CRISPR

Anapc10 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Anapc10 - mouse gene knockout kit via CRISPR

Anapc10 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Anapc10 - mouse gene knockout kit via CRISPR

Anapc10 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APC10 / ANAPC10

  Recombinant protein of human anaphase promoting complex subunit 10 (ANAPC10)  
APC10 / ANAPC10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ANAPC10 (mGFP-tagged) - Human anaphase promoting complex subunit 10 (ANAPC10), 200 uL, >10^7 TU/mL

Lenti ORF particles, ANAPC10 (mGFP-tagged) - Human anaphase promoting complex subunit 10 (ANAPC10), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ANAPC10 (Myc-DDK tagged) - Human anaphase promoting complex subunit 10 (ANAPC10) , 200 uL, >10^7 TU/mL

Lenti ORF particles, ANAPC10 (Myc-DDK tagged) - Human anaphase promoting complex subunit 10 (ANAPC10) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Anapc10 (GFP-tagged) - Mouse anaphase promoting complex subunit 10 (Anapc10), 200 uL, >10^7 TU/mL

Lenti ORF particles, Anapc10 (GFP-tagged) - Mouse anaphase promoting complex subunit 10 (Anapc10), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Anapc10 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 10 (Anapc10), 200 uL, >10^7 TU/mL

Lenti ORF particles, Anapc10 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 10 (Anapc10), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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