APC11 / ANAPC11 antibody

Principal name

APC11 / ANAPC11 antibody

Alternative names for APC11 / ANAPC11 antibody

Anaphase-promoting complex subunit 11, HSPC214, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein

SwissProt ID

Q3ZCF6 (Bovin), Q9CPX9 (Mouse), Q9NYG5 (Human)

Gene ID

51529 (ANAPC11), 100131844

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of APC11 / ANAPC11 antibody

Comprising more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to APC and activates APC’s cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC; thus preventing APC activation. APC11 is a RING-H2 finger protein that allows for the synthesis of multiubiquitin chains in the presence of Ubiquitin carrier protein 4 (Ubc4) and ubiquitin conjugating enzyme (E2). In addition, a heterodimeric complex of either Ubc4 or UbcH10 with APC11 and APC2 catalyzes the ubiquitination of human securin and cyclin B1.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APC11 / ANAPC11 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506321) Mouse IgG1 OTI1F2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APC11 / ANAPC11 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506323) Mouse IgG1 OTI3F5 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

APC11 / ANAPC11 (Center) antibody

Western blot analysis of APC11 in 3T3 cell tissue lysate with APC11 antibody at (A) 1 and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APC11 / ANAPC11 antibody

TA326323 (1µg/ml) staining of Cerebral Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APC11 / ANAPC11 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506235) Mouse IgG1 OTI1A8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APC11 / ANAPC11 antibody

Anti-ANAPC11 mouse monoclonal antibody (TA506300) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC11(RC200097). Mouse IgG2a OTI1B5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APC11 / ANAPC11 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506325) Mouse IgG1 OTI3C4 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APC11 / ANAPC11 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506326) Mouse IgG1 OTI1B11 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APC11 / ANAPC11 control peptide

APC11 / ANAPC11
50 µg / €160.00
  Acris Antibodies GmbH

APC11 / ANAPC11 antibody

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the mdifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see reference 3. Rabbit Serum Hu E, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

APC11 / ANAPC11 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and ANAPC11. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-ANAPC11 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 1B4-1A4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

APC11 / ANAPC11 antibody

APC11 / ANAPC11 Hu, Ms
0.1 ml / €560.00
  Abnova Taiwan Corp.

APC11 / ANAPC11 antibody

Immunohistochemistry-Paraffin: Apc11 Antibody [NBP1-90139] - Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

APC11 / ANAPC11 antibody

Immunohistochemistry-Paraffin: Apc11 Antibody [NBP1-90140] - staining of human kidney shows strong cytopalsmic positivity in tubular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APC11 / ANAPC11 (transcript variant 1)

APC11 / ANAPC11 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

APC11 / ANAPC11 (full length, N-term HIS tag, transcript variant 2)

APC11 / ANAPC11 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

APC11 / ANAPC11

APC11 / ANAPC11 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

APC11 / ANAPC11

APC11 / ANAPC11 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ANAPC11 overexpression lysate

ANAPC11 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ANAPC11 overexpression lysate

ANAPC11 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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