APC5 / ANAPC5 antibody

Principal name

APC5 / ANAPC5 antibody

Alternative names for APC5 / ANAPC5 antibody

Anaphase-promoting complex subunit 5, Cyclosome subunit 5

SwissProt ID

A1L1K3 (Rat), Q5ZKK3 (Chick), Q8BTZ4 (Mouse), Q9UJX4 (Human)

Gene ID

51433 (ANAPC5), 59008 (Anapc5), 288671

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of APC5 / ANAPC5 antibody

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APC5 / ANAPC5 antibody

  Rabbit polyclonal anti-ANAPC5 antibody  
Western blot analysis of extracts from HeLa cells, Jurkat cells and COLO205 cells, using ANAPC5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APC5 / ANAPC5 antibody

  Rabbit Polyclonal APC5 Antibody  
Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APC5 / ANAPC5 antibody

  Rabbit Polyclonal Anti-ANAPC5 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: NIH/3T3 cells, Primary antibody: (ANAPC5 Antibody) at dilution 1/187.5, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APC5 / ANAPC5 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APC5 / ANAPC5 (Center) antibody

ANAPC5 Antibody (Center) (Cat. #AP50172PU-N) western blot analysis in A549 cell line lysates (35 µg/lane).This demonstrates the ANAPC5 antibody detected the ANAPC5 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APC5 / ANAPC5 antibody

APC5 / ANAPC5 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

APC5 / ANAPC5 antibody

APC5 / ANAPC5 Rabbit Serum Hu, Ms, Rt E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

APC5 / ANAPC5 antibody

Western blot analysis of ANAPC expression in HEK293T (A); HeLa (B) whole cell lysates. Rabbit Aff - Purified Hu, Mky IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

APC5 / ANAPC5 antibody

Western blot analysis of ANAPC expression in HEK293T (A); HeLa (B) whole cell lysates. Rabbit Aff - Purified Hu, Mky IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

APC5 / ANAPC5 antibody

Western blot analysis of ANAPC expression in HEK293T (A); HeLa (B) whole cell lysates. Rabbit Aff - Purified Hu, Mky IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

ANAPC5 - human gene knockout kit via CRISPR

ANAPC5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ANAPC5 - human gene knockout kit via CRISPR

ANAPC5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ANAPC5 - human gene knockout kit via CRISPR

ANAPC5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ANAPC5 - human gene knockout kit via CRISPR

ANAPC5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Anapc5 - mouse gene knockout kit via CRISPR

Anapc5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Anapc5 - mouse gene knockout kit via CRISPR

Anapc5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Anapc5 - mouse gene knockout kit via CRISPR

Anapc5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Anapc5 - mouse gene knockout kit via CRISPR

Anapc5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APC5 / ANAPC5 (transcript variant 1)

  Recombinant protein of human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 1  
APC5 / ANAPC5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

APC5 / ANAPC5

APC5 / ANAPC5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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