APEX1 / REF-1 antibody

Principal name

APEX1 / REF-1 antibody

Alternative names for APEX1 / REF-1 antibody

APE, APEX, APX, HAP1, REF1, APEN, Apurinic-apyrimidinic endonuclease 1, APEX nuclease, Protein REF-1

SwissProt ID

P23196 (Bovin), P27695 (Human), P28352 (Mouse), P43138 (Rat)

Gene ID

328 (APEX1)

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Neutralisation (NEUT), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of APEX1 / REF-1 antibody

Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases.
APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.

General readings

1. Evans, A.R. et al. Going APE over Ref-1. Mutation Res. 461: 83-108, 2000.
2. Hedley, D., et al. Up-Regulation of the Redox Mediators Thioredoxin and Apurinic/Apyrimidinic Excision (APE)/Ref-1 in Hypoxic Microregions of Invasive Cervical Carcinomas, Mapped Using Mutispectral, Wide-Field Fluorescence Image Analysis. Am. J. Pathol. 164: 557-565, 2004. (Immunofluoresence, Human)
See individual datasheets 1. Zou, Gang-Ming., et al. Publishaheadofprint: Ape1 regulates hematopoietic differentation of embryonic stem cells through its redox functional domain. 10.1182/blood-2006-08-044172, 2006.
2. Zou, G., et al. Apel regulates hematopoietic differentiation of embryonic stem cells through its redox functional domain. Blood, Mar 2007; 109: 1917 - 1922.
3. Briegert, M., et al. Human Monocytes, but not Dendritic Cells Derived from Them, Are Defective in Base Excision Repair and Hypersensitive to Methylating Agents. Cancer Res. 2007 67: 26-31.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APEX1 / REF-1 antibody

Western Blot: APE1 Antibody TA301441 - Analysis of APE1 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APEX1 / REF-1 antibody

Western blot analysis of extracts of Hela cell and HepG2 cell, using APEX1 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APEX1 / REF-1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800330) Mouse IgG1 OTI1H9 Purified Can, Hu, Mky P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APEX1 / REF-1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800333) Mouse IgG2a OTI2A4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APEX1 / REF-1 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800334) Mouse IgG2a OTI6E10 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APEX1 / REF-1 antibody

Immunoperoxidase of purified MaxPab antibody to APEX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml] Mouse Serum Hu E(detection), ICC/IF, P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

APEX1 / REF-1 antibody

Immunoperoxidase of purified MaxPab antibody to APEX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

APEX1 / REF-1 antibody

Western Blot: APE1 Antibody (13B8E5C2) TA301765 - Ovarian Cancer cell lines. Mouse IgG2b 13B8E5C2 Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

APEX1 / REF-1 (N-term) antibody

TA302643 (0.3µg/ml) staining of A431 (A), HeLa (B) and MCF7 (C) nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APEX1 / REF-1 antibody

Sample (30 ug of whole cell lysate). A: A549. 10% SDS PAGE. TA308774 diluted at 1:3000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APEX1 / REF-1 antibody

Predicted band size: 36 kDa . Positive control: Hela, 293T and A431 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: A431 cell lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APEX1 / REF-1 antibody

Predicted band size: 36 kDa . Positive control: Hela cell and human liver cancer tissue, Raji, Jurkat, 293T and PC3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane2: Human liver cancer tissue lysate Lane3: Raji cell lysate Lane4: Jurkat cell lysate Lane5: 293T cell lysate Lane6: PC3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APEX1 / REF-1 antibody

Western blot analysis of extracts from COLO205 cells and HeLa cells, using APEX1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APEX1 / REF-1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800336) Mouse IgG2b OTI4D9 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

APEX1 / REF-1 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG 2104 Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APEX1 / REF-1 (N-term) antibody

Confocal Immunofluorescent analysis of APEX1 Antibody (N-term) Cat.-No AP12510PU-N with hela cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APEX1 / REF-1 (2-15) antibody

Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bt, Eq, Hu, Mky, Por, Prim, Rb E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APEX1 / REF-1 antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Serum Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

APEX1 / REF-1 antibody

APEX1 polyclonal antibody (A02), Lot # 060306JC01 Western Blot analysis of APEX1 expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

APEX1 / REF-1 antibody

Western Blot analysis of APEX1 expression in transfected 293T cell line (H00000328-T03) by APEX1 MaxPab polyclonal antibody.

Lane 1: APEX1 transfected lysate(35.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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