APG10L / ATG10 antibody

Principal name

APG10L / ATG10 antibody

Alternative names for APG10L / ATG10 antibody

Autophagy-related protein 10

SwissProt ID

Q8R1P4 (Mouse), Q9H0Y0 (Human)

Gene ID

83734 (ATG10), 66795 (Atg10)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of APG10L / ATG10 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is ATG10, an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.

General readings

Nemoto T., et al. (2003) J Bio Chem278:39517-39526Shin J H., et al. (2009) Mol Cells. 27: 37-74.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG10L / ATG10 (C-term) antibody

  Rabbit Polyclonal ATG10 Antibody  
Western blot analysis of ATG10 in SK-N-SH cell lysate with ATG10 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG10L / ATG10 antibody

  Rabbit Polyclonal Anti-ATG10 Antibody  
WB Suggested Anti-ATG10 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

APG10L / ATG10 antibody

  Rabbit Polyclonal Anti-ATG10 Antibody  
Gel: 10%SDS-PAGE, Lysate: 50 ug, Lane: Mouse liver tissue, Primary antibody: (ATG10 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

ATG10 - human gene knockout kit via CRISPR

ATG10 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ATG10 - human gene knockout kit via CRISPR

ATG10 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG10 - human gene knockout kit via CRISPR

ATG10 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG10 - human gene knockout kit via CRISPR

ATG10 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg10 - mouse gene knockout kit via CRISPR

Atg10 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Atg10 - mouse gene knockout kit via CRISPR

Atg10 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg10 - mouse gene knockout kit via CRISPR

Atg10 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg10 - mouse gene knockout kit via CRISPR

Atg10 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APG10L / ATG10 (transcript variant 3)

  Recombinant protein of human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3  
APG10L / ATG10 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

APG10L / ATG10 (1-220, His-tag)

APG10L / ATG10 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

APG10L / ATG10 (1-220, His-tag)

APG10L / ATG10 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

ATG10 overexpression lysate

ATG10 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ATG10 (mGFP-tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, ATG10 (mGFP-tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ATG10 (mGFP-tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, ATG10 (mGFP-tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ATG10 (Myc-DDK tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, ATG10 (Myc-DDK tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, ATG10 (Myc-DDK tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3 , 200 uL, >10^7 TU/mL

Lenti ORF particles, ATG10 (Myc-DDK tagged) - Human ATG10 autophagy related 10 homolog (S. cerevisiae) (ATG10), transcript variant 3 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Atg10 (GFP-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268), 200 uL, >10^7 TU/mL

Lenti ORF particles, Atg10 (GFP-tagged) - Mouse autophagy-related 10 (yeast) (cDNA clone MGC:30984 IMAGE:5249268), 200 uL, >10^7 TU/mL  
200 µl / €790.00
  OriGene Technologies, Inc.

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