APG10L / ATG10 antibody

Principal name

APG10L / ATG10 antibody

Alternative names for APG10L / ATG10 antibody

Autophagy-related protein 10

SwissProt ID

Q8R1P4 (Mouse), Q9H0Y0 (Human)

Gene ID

83734 (ATG10)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P)

Background of APG10L / ATG10 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is ATG10, an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.

General readings

Nemoto T., et al. (2003) J Bio Chem278:39517-39526Shin J H., et al. (2009) Mol Cells. 27: 37-74.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG10L / ATG10 (C-term) antibody

Western blot analysis of ATG10 in SK-N-SH cell lysate with ATG10 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG10L / ATG10 antibody

WB Suggested Anti-ATG10 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

APG10L / ATG10 antibody

Confocal microscopy on immunofluorescently detected ATG10 in cytospin-isolated human white blood cells using rabbit ATG10 polyclonal antibody ( Cat # PAB0730 ) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG10 appear in red. The cells were then counter stained with Hoechst Dye ( blue colour ) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hr, washed then 3x, and the secondary ( Goat anti Rb 1 : 100 alexa 546 conjugate ) was incubated for 30 minutes. Magnification 80X. Rabbit Hu C, ICC/IF
0.1 ml / €390.00
  Abnova Taiwan Corp.

APG10L / ATG10 antibody

APG10L / ATG10 Rabbit Hu, Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

APG10L / ATG10 antibody

Western blot analysis of ATG10 in SK-N-SH cell lysate with ATG10 polyclonal antibody ( Cat # PAB13017 ) at ( A ) 0.5, ( B ) 1 and ( C ) 2 µg/ml. Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

APG10L / ATG10 antibody

Human HepG2; WB Suggested Anti-ATG10 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ATG10 antibody - C-terminal region (ARP54274_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

APG10L / ATG10 antibody

Western Blot: ATG10 Antibody [NBP1-56625] - This Anti-ATG10 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

APG10L / ATG10 antibody

Western Blot: Atg10 Antibody [NBP1-76386] - Analysis of ATG10 in SK-N-SH cell lysate with ATG10 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

APG10L / ATG10 antibody

Western Blot: Atg10 Antibody (EPR4804) [NBP1-95893] (A) HeLa, (B) K562, and (C) 293T cell lysates using anti-Atg10. Rabbit EPR4804 Purified Hu IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APG10L / ATG10 (transcript variant 3)

APG10L / ATG10 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

APG10L / ATG10 (1-220, His-tag)

APG10L / ATG10 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

APG10L / ATG10 (1-220, His-tag)

APG10L / ATG10 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

APG10L / ATG10

APG10L / ATG10 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

APG10L / ATG10

APG10L / ATG10 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ATG10 overexpression lysate

ATG10 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

15 Item(s)

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