APG3 / ATG3 antibody

Principal name

APG3 / ATG3 antibody

Alternative names for APG3 / ATG3 antibody

APG3L, Autophagy-related protein 3, APG3-like, Protein PC3-96

SwissProt ID

Q0VCL3 (Bovin), Q6AZ50 (Rat), Q9CPX6 (Mouse), Q9NT62 (Human)

Gene ID

64422 (ATG3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of APG3 / ATG3 antibody

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

General readings

Tanida I., et al. (2002) J Biol Chem. 277: 13739-13744.Yamada Y., et al. (2007) J Biol Chem. 282: 8036-8043

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG3 / ATG3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503161) Mouse IgG1 OTI2C12 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APG3 / ATG3 antibody

Anti-ATG3 mouse monoclonal antibody (TA503299) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3(RC203453). Mouse IgG1 OTI3G3 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APG3 / ATG3 antibody

Anti-ATG3 mouse monoclonal antibody (TA503301) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3(RC203453). Mouse IgG2a OTI3C6 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APG3 / ATG3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503346) Mouse IgG2a OTI3H2 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

APG3 / ATG3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503370) Mouse IgG1 OTI4F6 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APG3 / ATG3 (Center) antibody

Western blot analysis of ATG3 in Mouse kidney tissue Lysate with ATG3 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG3 / ATG3 antibody

Western blot analysis of extracts of various cell lines, using ATG3 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG3 / ATG3 antibody

Anti-ATG3 mouse monoclonal antibody (TA503383) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3(RC203453). Mouse IgG2a OTI3H1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APG3 / ATG3 antibody

Anti-ATG3 mouse monoclonal antibody (TA503384) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3(RC203453). Mouse IgG2a OTI2B9 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

APG3 / ATG3 antibody

APG3 / ATG3 Mouse IgG2b AT10G1 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

APG3 / ATG3 antibody

APG3 / ATG3 Mouse IgG2b AT10G1 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

APG3 / ATG3 antibody

APG3 / ATG3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

APG3 / ATG3 antibody

ATG3 monoclonal antibody (M04), clone 1G3. Western Blot analysis of ATG3 expression in HepG2(Cat # L019V1 ). Mouse IgG2b 1G3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

APG3 / ATG3 antibody

ATG3 monoclonal antibody (M06), clone 1F7 Western Blot analysis of ATG3 expression in Jurkat ( Cat # L017V1 ). Mouse IgG2b 1F7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

APG3 / ATG3 antibody

Confocal microscopy on immunofluorescently detected ATG3 in cytospin - isolated human white blood cells using rabbit ATG3 polyclonal antibody ( Cat # PAB0740 ) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye ( blue colour ) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hour, washed then 3x, and the secondary ( Goat anti Rb 1 : 100 alexa 546 conjugate ) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line using rabbit ATG3 polyclonal antibody ( Cat # PAB0740 ) . Rabbit Hu, Rt C, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

APG3 / ATG3 antibody

APG3 / ATG3 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

APG3 / ATG3 antibody

APG3 / ATG3 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APG3 / ATG3

APG3 / ATG3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

APG3 / ATG3

APG3 / ATG3 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

APG3 / ATG3 (1-314, His-tag)

APG3 / ATG3 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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