APG4B / ATG4B antibody

Principal name

APG4B / ATG4B antibody

Alternative names for APG4B / ATG4B antibody

Autophagin-1, AUTL1, KIAA0943, Cysteine protease ATG4B, Autophagy-related protein 4 homolog B, Autophagy-related cysteine endopeptidase 1, AUT-like 1 cysteine endopeptidase

SwissProt ID

Q6PZ02 (Chick), Q6PZ03 (Bovin), Q8BGE6 (Mouse), Q9Y4P1 (Human)

Gene ID

23192 (ATG4B), 66615 (Atg4b)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of APG4B / ATG4B antibody

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignt tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. ATG4B is a member of the autophagin protein family, it is also desigted as a member of the C-54 family of cysteine proteases. Alterte transcriptiol splice variants, encoding different isoforms, have been characterized.Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignt tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also desigted as a member of the C-54 family of cysteine proteases. Alterte transcriptiol splice variants, encoding different isoforms, have been characterized.

General readings

Sugawara K., et al. (2005) J. Biol. Chem. 280:40058-40065Kumanomidou T., et al. (2006) J. Mol. Biol. 355:612-618

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG4B / ATG4B (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APG4B / ATG4B antibody

  Rabbit polyclonal anti-ATG4B antibody  
Western blot analysis of extracts from Jurkat cells, using ATG4B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B antibody

  Anti-APG4B Rabbit Polyclonal Antibody  
Predicted band size: 43kDa. Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B antibody

  Rabbit polyclonal anti-ATG4B antibody (Center)  
ATG4B Antibody (Center) (Cat. #TA328128) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ATG4B antibody detected the ATG4B protein (arrow). Rabbit Purified Bov, Chk, Hu, Ms, Ze WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B antibody

  Rabbit Polyclonal Anti-ATG4B Antibody  
WB Suggested Anti-APG4B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Raji cell lysateATG4B is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B antibody

  Rabbit Polyclonal Anti-ATG4B Antibody  
WB Suggested Anti-ATG4B Antibody; Titration: 2.5 ug/ml; Positive Control: RajiATG4B is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B antibody

  Rabbit Polyclonal ATG4B Antibody  
Western Blot: ATG4B Antibody [TA337038] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using TA337038 at 2 ug/ml. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B antibody

  Rabbit Polyclonal Anti-ATG4B Antibody - N-terminal region  
WB Suggested Anti-ATG4B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B antibody

  Rabbit Polyclonal Anti-ATG4B Antibody  
Western blot analysis on Jurkat cell lysate using ATG4B Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B antibody

  Rabbit Polyclonal Anti-ATG4B Antibody  
Western blot analysis of ATG4B in 3T3 cell lysate with ATG4B antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG4B / ATG4B (264-293) antibody

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Autophagy AAPG4B / ATG4B Antibody Cat.-No AP32207PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APG4B / ATG4B antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using ATG4B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €230.00
  Assay Biotech

APG4B / ATG4B antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using ATG4B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

ATG4B - human gene knockout kit via CRISPR

ATG4B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ATG4B - human gene knockout kit via CRISPR

ATG4B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG4B - human gene knockout kit via CRISPR

ATG4B - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG4B - human gene knockout kit via CRISPR

ATG4B - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg4b - mouse gene knockout kit via CRISPR

Atg4b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Atg4b - mouse gene knockout kit via CRISPR

Atg4b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg4b - mouse gene knockout kit via CRISPR

Atg4b - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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