APG4D / ATG4D antibody

Principal name

APG4D / ATG4D antibody

Alternative names for APG4D / ATG4D antibody

AUTL4, Autophagin-4, Cysteine protease ATG4D, Autophagy-related protein 4 homolog D, Autophagy-related cysteine endopeptidase 4, AUT-like 4 cysteine endopeptidase

SwissProt ID

Q684M2 (Pig), Q86TL0 (Human), Q8BGV9 (Mouse)

Gene ID

84971 (ATG4D)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Macaque, Bov (Bovine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Sheep, Goat, Mouse

Available applications

Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of APG4D / ATG4D antibody

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.; FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.; ENZYME REGULATION: Inhibited by N-ethylmaleimide; SUBCELLULAR LOCATION: Cytoplasm; TISSUE SPECIFICITY: Mainly expressed in skeletal muscle and, to a lower extent, in testis.;

General readings

"Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." Marino G., Uria J.A., Puente X.S., Quesada V., Bordallo J., Lopez-Otin C. J. Biol. Chem. 278:3671-3678(2003)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG4D / ATG4D (227-257) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APG4D / ATG4D antibody

Western blot analysis of extracts from K562 cells, using ATG4D antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APG4D / ATG4D antibody

ATG4D/MB10250 antibody (Cat. #TA324341) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ATG4D/MB10250  antibody detected the ATG4D/MB10250  protein (arrow). Mouse IgG1 222CT15.4.1 Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG4D / ATG4D antibody

WB Suggested Anti-ATG4D Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HCT15 cell lysate Rabbit Aff - Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

APG4D / ATG4D antibody

Western blot analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG4D / ATG4D (N-term) antibody

Formalin Fixed, Paraffin Embedded Human skeletal muscle stained with ATG4D Antibody (N-term) Cat.-No AP32159PU-N followed by peroxidase conjugation to the secondary antibody and DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APG4D / ATG4D antibody

APG4D / ATG4D Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

APG4D / ATG4D antibody

APG4D / ATG4D Rabbit Serum Hu, Ms C, P, WB
0.15 ml / €400.00
  Novus Biologicals Inc.

APG4D / ATG4D antibody

APG4D / ATG4D Sheep Serum Hu C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

APG4D / ATG4D antibody

Immunohistochemistry: ATG4D Antibody [NBP1-33488] - Immunohistochemical analysis of ATG4D on paraffin-embedded SAS xenograft, using NBP1-33488. Rabbit IgG Hu P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APG4D / ATG4D

APG4D / ATG4D Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

APG4D / ATG4D

APG4D / ATG4D Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

12 Item(s)

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