APG5L / ATG5 antibody

Principal name

APG5L / ATG5 antibody

Alternative names for APG5L / ATG5 antibody

ASP, APG5-like, Autophagy protein 5, Apoptosis-specific protein

SwissProt ID

Q3MQ04 (Pig), Q3MQ06 (Rat), Q3MQ24 (Bovin), Q99J83 (Mouse), Q9H1Y0 (Human), Q9W3R7 (Drome)

Gene ID

9474 (ATG5), 11793 (Atg5)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Xen (Xenopus), African clawed frog, Por (Porcine), Ze (Zebrafish), Prim (Primate), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P)

Background of APG5L / ATG5 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

General readings

1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated
induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4. Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem. 2007; 282:37298-302.
5. Jounai N, Takeshita F, Kobiyama K, et al. The Atg5-Atg12 conjugate associates with innate antiviral immune responses. Proc. Natl. Acad. Sci. USA 2007; 104:14050-5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG5L / ATG5 antibody

  Rabbit Polyclonal Antibody against ATG5  
Detection of ATG5 in mouse wildtype ES cell lysate (Lane 1). Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Rabbit IgG Purified Bov, Hu, Ms, Por, Prim, Rt, Xen, Ze ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APG5L / ATG5 (N-term) antibody

  Rabbit polyclonal ATG5 Antibody (N-term)  
The anti-APG5L Pab (Cat. #TA324633) is used in Western blot to detect APG5L in mouse brain tissue lysate. Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG5L / ATG5 antibody

  Rabbit Polyclonal Anti-ATG5 Antibody  
WB Suggested Anti-ATG5 Antibody Titration: 1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

APG5L / ATG5 (N-term) antibody

  Rabbit Polyclonal ATG5 Antibody  
Western blot analysis of ATG5 in rat spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG5L / ATG5 (N-term) antibody

  Chicken Polyclonal ATG5 Antibody  
Western blot analysis of ATG5 in mouse spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 2 ug/ml. Chicken Ig Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG5L / ATG5 antibody

  Rabbit polyclonal APG5 Antibody  
ATG5 Antibody (Cat.#TA324634) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the ATG5 antibody detected the ATG5 protein (arrow). Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG5L / ATG5 antibody

  Rabbit Polyclonal ATG5 Antibody  
Western Blot: ATG5 Antibody [TA336965] - Analysis of human APGL5 in T98G cell lysate in the 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/ml. Rabbit Purified Bov, Hu, Ms, Por, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG5L / ATG5 antibody

  Rabbit Polyclonal Anti-ATG5 Antibody  
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: NIH/3T3 cells, A172 cells, Primary antibody: (ATG5 Antibody) at dilution 1/475, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APG5L / ATG5 (incl. pos. control) antibody

Monoclonal antibody ATG5-7C6 detects the endogenous Atg5 as well as the overexpressed 33 kDa-protein in Jurkat cells. 
Cells were fixed with 4% paraformaldehyd for 5 min and permeabilized with 0.05% Saponin. After 10 min aceton-treatment, Atg5 was detected with mab ATG5-7C6 at 0.5 µg/ml.  Images by courtesy of Hans-Uwe Simon, MD, PhD, University of Bern. Mouse IgG1 7C6 Purified Can, Hu, Ms, Rt F, ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

APG5L / ATG5 (incl. pos. control) antibody

Whole lysate of serum starved SH-SY5Y cells was applied to SDS-PAGE and tranferred to a PVDF membrane. The Immunoblot was probed with the indicated monoclonal antibodies at 0.5 µg/ml for 1h at 15-22°C and developed with ECL (exposure time: 30 sec).
Lane M: Molecular Weight marker
Lane 1: AM20205PU-N (Clone 7C6)
Lane 2: AM20206PU-N (Clone 11C3) Mouse IgG1 11C3 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

APG5L / ATG5 (N-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bov, Hu, Ms, Por, Pufferfish, Rt, Ze P
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ATG5 - human gene knockout kit via CRISPR

ATG5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ATG5 - human gene knockout kit via CRISPR

ATG5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG5 - human gene knockout kit via CRISPR

ATG5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG5 - human gene knockout kit via CRISPR

ATG5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg5 - mouse gene knockout kit via CRISPR

Atg5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Atg5 - mouse gene knockout kit via CRISPR

Atg5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg5 - mouse gene knockout kit via CRISPR

Atg5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg5 - mouse gene knockout kit via CRISPR

Atg5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APG5L / ATG5

  Recombinant protein of human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5)  
APG5L / ATG5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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