APG7L / ATG7 antibody

Principal name

APG7L / ATG7 antibody

Alternative names for APG7L / ATG7 antibody

APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein

SwissProt ID

O95352 (Human), P38862 (Yeast), Q5ZKY2 (Chick), Q641Y5 (Rat), Q9D906 (Mouse)

Gene ID

10533 (ATG7), 312647 (Atg7), 74244

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of APG7L / ATG7 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG7L / ATG7 (501-531) antibody

Western blot analysis of anti-hAPG7L-R509 Pab (Cat. #TA324635) in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). hAPG7L-R509(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (C-term) antibody

Western blot analysis of APG7L (C-term) Pab  (Cat. #TA324636) in 293 cell line lysates transiently transfected with the ATG7 gene(2ug/lane). Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (C-term) antibody

Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (N-term) antibody

Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 antibody

Adrenal: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 (C-term) antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 (N-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 Control Peptide

APG7L / ATG7
50 µg / €160.00
  Acris Antibodies GmbH

APG7L / ATG7 Control Peptide

APG7L / ATG7
50 µg / €160.00
  Acris Antibodies GmbH

APG7L / ATG7 antibody

APG7L / ATG7 Rabbit Purified Hu E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

APG7L / ATG7 Control Peptide

APG7L / ATG7 Purified
50 µg / €200.00
  Acris Antibodies GmbH

APG7L / ATG7 antibody

Western Blot analysis of ATG7 expression in transfected 293T cell line (H00010533-T01) by ATG7 MaxPab polyclonal antibody.

Lane 1: ATG7 transfected lysate(77.33 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

APG7L / ATG7 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Western Blot analysis of ATG7 expression in transfected 293T cell line (H00010533-T02) by ATG7 MaxPab polyclonal antibody.

Lane 1: ATG7 transfected lysate(78.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Confocal microscopy on cytospin - isolated human blood cells. A : Neutrophils seem to be stained. B : The cells were double - stained for ATG7 appearing in red and Myeloperoxidase ( MPO ) appearing in green. Using rabbit ATG7 polyclonal antibody ( Cat # PAB0742 ) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye ( blue colour ) . Here, the merged picture is presented. Observations : No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line. Rabbit Hu, Rt C, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Western blot analysis of Atg7 expression in Jurkat cell lysates. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Immunocytochemistry of ATG7 in MCF-7 cells with ATG7 polyclonal antibody ( Cat # PAB13169 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Immunocytochemistry of ATG7 in L1210 cells with ATG7 polyclonal antibody ( Cat # PAB13170 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APG7L / ATG7 (transcript variant 1)

APG7L / ATG7 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

APG7L / ATG7

APG7L / ATG7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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