APG7L / ATG7 antibody

Principal name

APG7L / ATG7 antibody

Alternative names for APG7L / ATG7 antibody

APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein

SwissProt ID

O95352 (Human), P38862 (Yeast), Q5ZKY2 (Chick), Q641Y5 (Rat), Q9D906 (Mouse)

Gene ID

10533 (ATG7), 312647 (Atg7)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Macaque

Available hosts

Rabbit, Sheep, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection))

Background of APG7L / ATG7 antibody

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.; FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.; SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.; SUBCELLULAR LOCATION: Cytoplasm.; TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.; DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.

General readings

Yuan,W., Stromhaug,P.E. and Dunn,W.A. Jr. (1999) Glucose-induced autophagy of peroxisomes in Pichia pastoris requires a unique E1-like protein. Mol. Biol. Cell 10 (5), 1353-1366.
Tanida I, Komatsu M, Ueno T, Kominami E. (2003) GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3. Biochem
Biophys Res Commun. 300(3):637-44.

  1. Quick-Filters:
  2. Highly Validated (2)

Items 1 to 20 of 33 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG7L / ATG7 (501-531) antibody

Western blot analysis of anti-hAPG7L-R509 Pab (Cat. #TA324635) in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). hAPG7L-R509(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (C-term) antibody

Western blot analysis of APG7L (C-term) Pab  (Cat. #TA324636) in 293 cell line lysates transiently transfected with the ATG7 gene(2ug/lane). Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (C-term) antibody

Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (N-term) antibody

Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 antibody

Adrenal: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 (C-term) antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 (N-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 Control Peptide

APG7L / ATG7
50 µg / €160.00
  Acris Antibodies GmbH

APG7L / ATG7 Control Peptide

APG7L / ATG7
50 µg / €160.00
  Acris Antibodies GmbH

APG7L / ATG7 antibody

APG7L / ATG7 Rabbit Purified Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

APG7L / ATG7 Control Peptide

APG7L / ATG7 Purified
50 µg / €190.00
  Acris Antibodies GmbH

APG7L / ATG7 antibody

Western Blot analysis of ATG7 expression in transfected 293T cell line (H00010533-T01) by ATG7 MaxPab polyclonal antibody.

Lane 1: ATG7 transfected lysate(77.33 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

APG7L / ATG7 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Western Blot analysis of ATG7 expression in transfected 293T cell line (H00010533-T02) by ATG7 MaxPab polyclonal antibody.

Lane 1: ATG7 transfected lysate(78.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Confocal microscopy on cytospin - isolated human blood cells. A : Neutrophils seem to be stained. B : The cells were double - stained for ATG7 appearing in red and Myeloperoxidase ( MPO ) appearing in green. Using rabbit ATG7 polyclonal antibody ( Cat # PAB0742 ) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye ( blue colour ) . Here, the merged picture is presented. Observations : No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line. Rabbit Hu, Rt C, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Western blot analysis of Atg7 expression in Jurkat cell lysates. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Immunocytochemistry of ATG7 in MCF-7 cells with ATG7 polyclonal antibody ( Cat # PAB13169 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Immunocytochemistry of ATG7 in L1210 cells with ATG7 polyclonal antibody ( Cat # PAB13170 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

APG7L / ATG7 antibody

Immunohistochemistry: ATG7 Antibody (EP1759Y) [NB110-55474] - Immunohistochemical analysis of paraffin-embedded human liver using anti-Atg7 Rabbit IgG EP1759Y Purified Hu F, ICC/IF, IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

APG7L / ATG7 antibody

APG7L / ATG7 Sheep IgG Purified Hu, Ms, Rt P
0.5 mg / €400.00
  Novus Biologicals Inc.

Items 1 to 20 of 33 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn