APG7L / ATG7 antibody

Principal name

APG7L / ATG7 antibody

Alternative names for APG7L / ATG7 antibody

APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein

SwissProt ID

O95352 (Human), P38862 (Yeast), Q5ZKY2 (Chick), Q641Y5 (Rat), Q9D906 (Mouse)

Gene ID

10533 (ATG7), 312647 (Atg7), 74244

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of APG7L / ATG7 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APG7L / ATG7 (501-531) antibody

  Rabbit polyclonal ATG7 Antibody  
Western blot analysis of anti-hAPG7L-R509 Pab (Cat. #TA324635) in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). hAPG7L-R509(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (C-term) antibody

  Rabbit polyclonal ATG7 Antibody (C-term)  
Western blot analysis of APG7L (C-term) Pab  (Cat. #TA324636) in 293 cell line lysates transiently transfected with the ATG7 gene(2ug/lane). Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (C-term) antibody

  Rabbit Polyclonal APG7 Antibody  
Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 (N-term) antibody

  Rabbit Polyclonal APG7 Antibody  
Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APG7L / ATG7 antibody

Adrenal: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 (C-term) antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 (N-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APG7L / ATG7 antibody

APG7L / ATG7 Rabbit Purified Hu E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

APG7L / ATG7 Control Peptide

APG7L / ATG7 Purified
50 µg / €200.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ATG7 - human gene knockout kit via CRISPR

ATG7 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ATG7 - human gene knockout kit via CRISPR

ATG7 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG7 - human gene knockout kit via CRISPR

ATG7 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG7 - human gene knockout kit via CRISPR

ATG7 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg7 - mouse gene knockout kit via CRISPR

Atg7 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Atg7 - mouse gene knockout kit via CRISPR

Atg7 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg7 - mouse gene knockout kit via CRISPR

Atg7 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg7 - mouse gene knockout kit via CRISPR

Atg7 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APG7L / ATG7 (transcript variant 1)

  Recombinant protein of human ATG7 autophagy related 7 homolog (S. cerevisiae) (ATG7), transcript variant 1  
APG7L / ATG7 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ATG7 overexpression lysate

ATG7 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

ATG7-knockout Cell lysate

ATG7-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

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