APH1A antibody

Principal name

APH1A antibody

Alternative names for APH1A antibody

Gamma-secretase subunit APH-1A, APH-1A, Aph-1 alpha, PSF, Presenilin-stabilization factor, CGI-78, aph-1

SwissProt ID

Q8BVF7 (Mouse), Q96BI3 (Human)

Gene ID

51107 (APH1A), 327 (APEH)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Prim (Primate), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P), ChIP assay (ChIP)

Background of APH1A antibody

APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.

General readings

Spasic D, Raemaekers T, Dillen K, Declerck I, Baert V, Serneels L, Fullekrug J, Annaert W. Rer1p competes with APH-1 for binding to nicastrin and regulates gamma-secretase complex assembly in the early secretory pathway. J Cell Biol. 2007 Feb 26;176(5):629-40. PMID: 17325205

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APH1A antibody

TA303251 (0.05µg/ml) staining of Human Brain (Cerebral Cortex) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APH1A (N-term) antibody

Western blot analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APH1A (Center) antibody

Western blot analysis of APH1 in human brain tissue lysate with APH1 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APH1A antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APH1A antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Placenta. Rabbit Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APH1A (N-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APH1A Control Peptide

APH1A
50 µg / €160.00
  Acris Antibodies GmbH

APH1A Control Peptide

APH1A
50 µg / €160.00
  Acris Antibodies GmbH

APH1A antibody

Western blot analysis of APH1A polyclonal antibody ( Cat # PAB4887 ) in A2058 cell lysate ( 35 µg/lane ) . APH1A ( arrow ) was detected using the purified polyclonal antibody. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

APH1A antibody

APH1A polyclonal antibody ( Cat # PAB7304 ) ( 0.05 µg/ml ) staining of human brain ( cerebral cortex ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

APH1A antibody

Western blot analysis of extract from Neuro 2a cells, using APH1A polyclonal antibody ( Cat # PAB8483 ) . Lane 1: mock, lane 2: APH1A ( 247 a.a. ) and lane 3: APH1A ( 265 a. a. ) . Rabbit Hu WB
25 µg / €660.00
  Abnova Taiwan Corp.

APH1A antibody

Western blot analysis of APH1A in RAW264.7 cell lysate with APH1A polyclonal antibody ( Cat # PAB13237 ) at ( A ) 0.5, ( B ) 1 and ( C ) 2 µg/ml. Rabbit Hu, Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

APH1A antibody

Immunohistochemistry of APH1A in human brain tissue with APH1A polyclonal antibody ( Cat # PAB13238 ) at 5 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

APH1A antibody

APH1A Rabbit Aff - Purified Hu WB
25 µg / €630.00
  Funakoshi Co., Ltd.

APH1A antibody

NB100-61660 (0.05ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemilumine Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

APH1A antibody

Immunofluorescence: APH1A Antibody [NB100-74360] - shows immunofluorescence staining of APH-1 in human KNS-42 glioma cells using NB100-74360 Rabbit Aff - Purified Hu, Ms C, ICC/IF, IP, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

APH1A antibody

Western Blot: APH1a Antibody [NBP1-76401] - Analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. _x000d_ Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

APH1A antibody

Immunohistochemistry-Paraffin: APH1a Antibody [NBP1-77272] - Human brain tissue with APH1 antibody at 5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

APH1A overexpression lysate

APH1A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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