API5 / MIG8 antibody

Principal name

API5 / MIG8 antibody

Alternative names for API5 / MIG8 antibody

Protein XAGL, FIF, AAC-11, Apoptosis inhibitor 5, Fibroblast growth factor 2-interacting factor, Antiapoptosis clone 11 protein, Cell migration-inducing gene 8 protein

SwissProt ID

O35841 (Mouse), Q5ZMW3 (Chick), Q9BZZ5 (Human)

Gene ID

8539 (API5), 11800 (Api5)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of API5 / MIG8 antibody

Kit Component:
- KN301397G1, Api5 gRNA vector 1 in pCas-Guide vector
- KN301397G2, Api5 gRNA vector 2 in pCas-Guide vector
- KN301397D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Tewari M., Cancer Res. 57:4063-4069(1997).
Van den Berghe L., Mol. Endocrinol. 14:1709-1724(2000).
Olsen J.V., Cell 127:635-648(2006).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

API5 / MIG8 antibody

  Rabbit polyclonal anti-API-5 antibody  
Western blot analysis of extracts from 293 cells, Jurkat cells and HUVEC cells, using API-5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

API5 / MIG8 antibody

  Anti-API5 Rabbit Polyclonal Antibody  
Predicted band size: 57 kDa . Positive control: 293T and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lysates: 30 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

API5 / MIG8 antibody

  Anti-API5 Rabbit Polyclonal Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane 1-3: 293T cells, A549 cells, Hela cells, Primary antibody: API5 Antibody at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

API5 / MIG8 antibody

  Rabbit Polyclonal Anti-API5 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, 293T cells, Primary antibody: (API5 Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

API5 / MIG8 (C-term) antibody

Immunohistochemical analysis of paraffin embedded Human epidermic carinoma sections using API5 antibody AP23281PU-N Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

API5 / MIG8 (C-term) antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue using  API5 / MIG8 Antibody Cat.-No AP32280PU-N . Rabbit IgG Aff - Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

API5 / MIG8 antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using API-5 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €230.00
  Assay Biotech

API5 / MIG8 antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using API-5 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

API5 - human gene knockout kit via CRISPR

API5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

API5 - human gene knockout kit via CRISPR

API5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

API5 - human gene knockout kit via CRISPR

API5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

API5 - human gene knockout kit via CRISPR

API5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Api5 - mouse gene knockout kit via CRISPR

Api5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Api5 - mouse gene knockout kit via CRISPR

Api5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Api5 - mouse gene knockout kit via CRISPR

Api5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Api5 - mouse gene knockout kit via CRISPR

Api5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

API5 / MIG8 (transcript variant 3)

  Recombinant protein of human apoptosis inhibitor 5 (API5), transcript variant 3  
API5 / MIG8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

API5 overexpression lysate

API5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, API5 (mGFP-tagged)-Human apoptosis inhibitor 5 (API5), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, API5 (mGFP-tagged)-Human apoptosis inhibitor 5 (API5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, API5 (mGFP-tagged) - Human apoptosis inhibitor 5 (API5), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, API5 (mGFP-tagged) - Human apoptosis inhibitor 5 (API5), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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