API5 / MIG8 antibody

Principal name

API5 / MIG8 antibody

Alternative names for API5 / MIG8 antibody

Protein XAGL, FIF, AAC-11, Apoptosis inhibitor 5, Fibroblast growth factor 2-interacting factor, Antiapoptosis clone 11 protein, Cell migration-inducing gene 8 protein

SwissProt ID

O35841 (Mouse), Q5ZMW3 (Chick), Q9BZZ5 (Human)

Gene ID

8539 (API5)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of API5 / MIG8 antibody

Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death (apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

General readings

Tewari M., Cancer Res. 57:4063-4069(1997).
Van den Berghe L., Mol. Endocrinol. 14:1709-1724(2000).
Olsen J.V., Cell 127:635-648(2006).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

API5 / MIG8 antibody

Immunoperoxidase of monoclonal antibody to API5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG2b 1C2 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

API5 / MIG8 antibody

Western blot analysis of extracts from 293 cells, Jurkat cells and HUVEC cells, using API-5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

API5 / MIG8 antibody

Predicted band size: 57 kDa . Positive control: 293T and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lysates: 30 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

API5 / MIG8 antibody

Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane 1-3: 293T cells, A549 cells, Hela cells, Primary antibody: API5 Antibody at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

API5 / MIG8 (C-term) antibody

Immunohistochemical analysis of paraffin embedded Human epidermic carinoma sections using API5 antibody AP23281PU-N Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

API5 / MIG8 (C-term) antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue using  API5 / MIG8 Antibody Cat.-No AP32280PU-N . Rabbit IgG Aff - Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

API5 / MIG8 antibody

Immunohistochemical analysis of paraffin-embedded human epidermic carcinoma sections using API5 polyclonal antibody ( Cat # PAB7951 ) . Rabbit Hu, Ms, Rb, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

API5 / MIG8 antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using API-5 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

API5 / MIG8 antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using API-5 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

API5 / MIG8 antibody

API5 / MIG8 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

API5 / MIG8 antibody

Immunohistochemical analysis of API5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

API5 / MIG8 antibody

Immunohistochemical analysis of API5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

API5 / MIG8 antibody

Immunohistochemical analysis of API5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

API5 / MIG8 antibody

Western Blot and Immunoprecipitation: API5 Antibody [NBP1-46223]-  Whole cell  lysate from HeLa, 293T, and mouse NIH3T3 cells. API5 was also immunoprecipitated by rabbit anti-API5 antibody NBP1-46224. Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

API5 / MIG8 antibody

API5 / MIG8 Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

API5 / MIG8 antibody

API5 / MIG8 Rabbit IgG Aff - Purified Hu, Ms E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

API5 / MIG8 antibody

Immunohistochemistry-Paraffin: API5 Antibody [NBP1-89326] - Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

API5 / MIG8 (transcript variant 3)

API5 / MIG8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

API5 / MIG8

API5 / MIG8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

API5 / MIG8

API5 / MIG8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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