APOBEC3G antibody

Principal name

APOBEC3G antibody

Alternative names for APOBEC3G antibody

ARP9, CEM15, MDS019, APOBEC-3G, ARCD, DNA dC->dU-editing enzyme APOBEC-3G, APOBEC-related cytidine deaminase

SwissProt ID

Q9HC16 (Human), Q9HC16-2

Gene ID

60489 (APOBEC3G), 200316 (APOBEC3F)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of APOBEC3G antibody

APOBEC3G is a DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV1 infection) by triggering G-to-A hypermutation in the newly synthetized viral DNA. The replacements C-to-U in the minus strand DNA of HIV1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing.
APOBEC3G is involved in hepatitis B virus (HBV) resistance probably primarily by inhibiting viral pregenomic RNA packaging. This activity is independent of the cytidine deaminase activity, as the inactivated protein is able to inhibit HBV replication. During HBV infection, APOBEC3G does not seem to be involved in the editing of HBV DNA, but this might occur in particular cellular contexts or with specific viral strains.
APOBEC3G is a homodimer. It interacts with APOBEC3B, APOBEC3F and HIV1 VIF in a species specific manner. It is expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines. Normally not expressed in the liver, but it is possible that it is induced in the course of HBV infection, for instance under the influence of cytokines.
APOBEC3G is ubiquitinated in the presence of HIV1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin dependent proteasome pathway.
APOBEC3G inhibits also the infectivity of retroviral particles only distantly related to HIV1, such as the simian immunodeficiency virus (SIV), the equine infectious anemia virus (EIAV) and the murine leukemia virus (MLV).

General readings

Derse D, Hill SA, Princler G, Lloyd P, Heidecker G. Resistance of human T cell leukemia virus type 1 to APOBEC3G restriction is mediated by elements in nucleocapsid. Proc Natl Acad Sci U S A. 2007 Feb 13; [Epub ahead of print]. PMID: 17299050

  1. Quick-Filters:
  2. Highly Validated (1)

Items 1 to 20 of 33 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APOBEC3G antibody

Western blot analysis of extracts of Jurkat cell line, using APOBEC3G antibody. Rabbit IgG Purified Hu, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APOBEC3G (C-term) antibody

TA305732 (0.5ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APOBEC3G (N-term) antibody

Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 ug/ml. . Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APOBEC3G antibody

TA329669 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Goat Aff - Purified Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

APOBEC3G antibody

WB Suggested Anti-APOBEC3G Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Daudi cell lysate Rabbit IgG Purified Hu, Por P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

APOBEC3G antibody

WB Suggested Anti-APOBEC3G Antibody; Titration: 2.5 ug/ml; Positive Control: DaudiAPOBEC3G is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells Rabbit IgG Purified Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APOBEC3G antibody

Figure 1. Western analysis of cell lysates from PBMC cells using CEM15 abtibody (Cat. #AM11032PU-N). Mouse IgG1 34AT1147 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APOBEC3G (Center) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APOBEC3G (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

APOBEC3G (C-term) antibody

Immunohistochemistry Image: Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

APOBEC3G Control Peptide

APOBEC3G
50 µg / €160.00
  Acris Antibodies GmbH

APOBEC3G antibody

Immunohistochemical staining of human spleen with Rabbit anti Human AOPBEC3G (Cat.-No SP2151P) Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

APOBEC3G antibody

Immunohistochemical staining of human spleen with Rabbit anti Human AOPBEC3G (Cat.-No SP2151P) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €280.00
  Acris Antibodies GmbH

APOBEC3G antibody

APOBEC3G Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

APOBEC3G antibody

Western Blot analysis of APOBEC3G expression in transfected 293T cell line (H00060489-T03) by APOBEC3G MaxPab polyclonal antibody.

Lane 1: APOBEC3G transfected lysate(42.24 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

APOBEC3G antibody

Western Blot analysis of APOBEC3G expression in transfected 293T cell line (H00060489-T01) by APOBEC3G MaxPab polyclonal antibody.

Lane 1: APOBEC3G transfected lysate(46.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

APOBEC3G antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the APOBEC3G polyclonal antibody ( Cat # PAB2470 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

APOBEC3G antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the APOBEC3G polyclonal antibody ( Cat # PAB2472 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

APOBEC3G antibody

APOBEC3G polyclonal antibody ( Cat # PAB11417 ) ( 0.5 µg/ml ) staining of Daudi lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

APOBEC3G antibody

Immunohistochemical staining of human spleen using APOBEC3G polyclonal antibody ( Cat # PAB12912 ) at 1 µg/ml. Rabbit Hu P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Items 1 to 20 of 33 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn