Apolipoprotein E / Apo E antibody

Principal name

Apolipoprotein E / Apo E antibody

Alternative names for Apolipoprotein E / Apo E antibody

ApoE, Apo-E

SwissProt ID

P02649 (Human), P02650 (Rat), P08226 (Mouse), P18287 (Rabit), P18649 (Canfa), P18650 (Pig), Q03247 (Bovin)

Gene ID

348 (APOE), 11816 (Apoe)

Available reactivities

Ms (Mouse), Hst (Hamster), Hu (Human), Rt (Rat), Rb (Rabbit), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Immunodiffusion (ID), Western blot / Immunoblot (WB), Radioimmunoassay (R), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Dot blot (Dot)

Background of Apolipoprotein E / Apo E antibody

ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctiol glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnt lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnts, a subclass of HDL etc, and in cerebrospil fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.

General readings

Dodart JC, Marr RA, Koistinaho M, Gregersen BM, Malkani S, Verma IM, Paul SM. Gene delivery of human apolipoprotein E alters brain A{beta} burden in a mouse model of Alzheimer's disease. Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1211-6. Epub 2005 Jan 18.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Apolipoprotein E / Apo E antibody

Apolipoprotein E / Apo E Goat Purified Hu C, E, IP, P, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

Apolipoprotein E / Apo E antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-APOE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805091) Mouse IgG1 OTI1E6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Apolipoprotein E / Apo E antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-APOE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805285) Mouse IgG2b OTI1H6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Apolipoprotein E / Apo E antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-APOE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805356) Mouse IgG2b OTI3F1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Apolipoprotein E / Apo E antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-APOE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805357) Mouse IgG1 OTI3D3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Apolipoprotein E / Apo E antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and brain tissues (right) using ApoE mouse mAb with DAB staining. Mouse IgG1 1H4 Ascites Hu E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Apolipoprotein E / Apo E antibody

Human kidney.; Anti-APOE / Apolipoprotein E antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.; APOE antibody - N-terminal region (ARP54283_P050) in Human kidney. cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
50 µg / €350.00
  AVIVA Systems Biology

Apolipoprotein E / Apo E (C-term) antibody

TA302644 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

Apolipoprotein E / Apo E (C-term) antibody

Western blot analysis of lysate from human blood plasma tissue lysate, using APOE Antibody (C-term)(Cat. #TA302010). TA302010 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E (C-term) antibody

TA302644 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

Western Blot: ApoE4 Antibody (4E4) - Western blot analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using anti-ApoE4.  Image courtesy of Dr. Marko Roblek from the Medical University of Vienna. Mouse IgG1 Purified Hu E, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

Western blot analysis of ApoE4 using recombinant human ApoE4. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

Western blot analysis of extracts from K562 cells, using APOE antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

To detect hApoE3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hApoE3. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

To detect hApoE3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human ApoE3 (TA328252) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

Western Blot: ApoE Antibody (WUE-4) [TA336538] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, rat liver lysate, human plasma, and mo Mouse IgG1 WUE-4 Purified Hu, Ms C, E, F, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

Western Blot: ApoE Antibody (1H4) [TA336912] - Western blot analysis using ApoE mAb against HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 1H4 Purified Hu E, F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Apolipoprotein E / Apo E antibody

Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-APOE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805083) Mouse IgG1 OTI1D6 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Apolipoprotein E / Apo E antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOE (RC200395, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOE. Mouse IgG1 OTI6B9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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